Phage display : a practical approach / / edited by Tim Clackson, Henry B. Lowman

• Record Nr.: UNINA9910824041603321
• Pubbl/distr/stampa: Oxford ; ; New York, : Oxford University Press, c2004
• ISBN: 1-383-05013-9; 1-280-84529-5; 0-19-151645-7
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (357 p.)
• Collana: Practical approach series ; ; no. 266
• Altri autori (Persone): ClacksonTim; LowmanHenry B
• Disciplina: 579.26
• Soggetti: Bacteriophages; Viral proteins; Microbial biotechnology
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Previously issued in print: 2004.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Contents; Protocol list; List of abbreviations; List of contributors; 1 Introduction to phage biology and phage display; 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3 In vitro DNA recombination; 4 Phage selection strategies for improved affinity and specificity of proteins and peptides; 5 Rapid screening of phage displayed protein binding affinities by phage ELISA; 6 Identification of novel ligands for receptors using recombinant peptide libraries; 7 Substrate phage display; 8 Protease-based selection of stably folded proteins and protein domains from phage display libraries9 Phage display of zinc fingers and other nucleic acid-binding motifs; 10 In vivo and ex vivo selections using phage-displayed libraries; 11 Screening phage libraries with sera; 12 Interaction cloning using cDNA libraries displayed on phage; 13 Phage antibody libraries; 14 Affinity maturation of phage antibodies; Appendix: Non-standard suppliers; Index
• Sommario/riassunto: Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is ; widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display - A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analysing the results.