Drosophila protocols / / edited by William Sullivan, Michael Ashburner, R. Scott Hawley

• Record Nr.: UNINA9910823916903321
• Pubbl/distr/stampa: Cold Spring Harbor, N.Y., : Cold Spring Harbor Laboratory Press, c2000
• Descrizione fisica: xiv, 697 p. : ill. (some col.)
• Altri autori (Persone): SullivanWilliam <1957->; AshburnerM; HawleyR. Scott
• Disciplina: 595.77/4
• Soggetti: Drosophila
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Intro -- Contents -- Preface -- Abbreviations -- Preparation and Analysis of Drosophila Mitotic Chromosomes -- In Situ Hybridization to Somatic Chromosomes -- BrdU Labeling of Chromosomes -- Analysis of Meiosis in Fixed and Live Oocytes by Light Microscopy -- Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster -- Preparation and Analysis of Polytene Chromosomes -- In Situ Hybridization to Polytene Chromosomes -- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining -- Fluorescent Analysis of Drosophila Embryos -- Imaginal Discs -- The Neuromuscular Junction -- Histological Techniques for the Drosophila Eye Part I: Larva and Pupa -- Histological Techniques for the Drosophila Eye Part II: Adult -- Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy -- Electrophysiological Approaches to the Neuromusculature -- Functional Assays of the Peripheral and Central Nervous Systems -- GFP and Other Reporters -- Quantitative Microinjection of Drosophila Embryos -- Targeted Disruption of Gene Function in Drosophila by RNA Interference -- Drosophila Cell Culture and Transformation -- Generating Antibodies against Drosophila Proteins -- Photoactivated Gene Expression for Cell- fate Mapping and Cell Manipulation -- Recovery of DNA Sequences Flanking P- element Insertions: Inverse PCR and Plasmid Rescue -- GAL4- mediated Ectopic Gene Expression in ; Drosophila -- Functional Cell Ablation -- Preparation of DNA from E. coli Cells Containing Bacteriophage P1 Clones -- Drosophila Microarrays: From Arrayer Construction to Hybridization -- Using Drosophila Genome Databases -- Preparation and Analysis of High- molecular- weight DNA in Drosophila -- Culturing Large Populations of Drosophila for Protein Biochemistry*.; Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis -- Preparation of Membrane Proteins from Drosophila Embryos -- Preparing Cytoplasmic Extracts from Drosophila Embryos -- Immunoblotting of Proteins from Single Drosophila Embryos -- Laboratory Culture of Drosophila -- Preparation of Larval and Adult Cuticles for Light Microscopy -- Exposing Drosophila to Neuroactive Drugs -- 1989 Table of Contents from Drosophila: A Laboratory Manual -- Anatomical Drawings of Drosophila -- Solutions and Reagents -- Cautions -- Suppliers -- Trademarks -- Index.