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1. |
Record Nr. |
UNIBAS000002601 |
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Autore |
Fraser, Marie |
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Titolo |
In Stevenson's Samoa / by Marie Fraser |
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Pubbl/distr/stampa |
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New York : Macmillan, 1895 |
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Descrizione fisica |
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VI, 190 p., [1] c. di tav. ; 17 cm. |
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Disciplina |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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2. |
Record Nr. |
UNISALENTO991001661699707536 |
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Autore |
Ghia, Lucio |
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Titolo |
I contratti di finanziamento dell'impresa / Lucio Ghia |
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Pubbl/distr/stampa |
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Milano : Giuffrè |
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Roma : Luiss University press, 1997-2005 |
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ISBN |
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Descrizione fisica |
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Collana |
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Collana di studi economico aziendali; 27 |
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Disciplina |
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Soggetti |
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Imprese - Finanziamenti |
Leasing |
Factoring |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Nominalmente 2. volume, in realtà nuova edizione dell'opera del 1997 |
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3. |
Record Nr. |
UNINA9910815366903321 |
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Titolo |
Advanced delivery and therapeutic applications of RNAi / / edited by Kun Cheng, Ram I. Mahato |
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Pubbl/distr/stampa |
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Chichester, West Sussex, : John Wiley & Sons Inc., 2013 |
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ISBN |
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9781118610732 |
1118610733 |
9781118610749 |
1118610741 |
9781299465282 |
1299465285 |
9781118610756 |
111861075X |
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Edizione |
[2nd ed.] |
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Descrizione fisica |
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1 online resource (535 p.) |
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Classificazione |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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RNA - Therapeutic use |
Small interfering RNA - Therapeutic use |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Advanced Delivery and Therapeutic Applications of RNAi; Contents; Preface; Contributors; About the Editors; Part 1: Introduction and Basics of RNAi; 1 Mechanisms and Barriers to RNAi Delivery; 1.1 Introduction; 1.2 Barriers to Systemic RNAi Delivery; 1.3 Rational Design to Improve RNAi Efficacy; 1.4 Chemical Modifications to Enhance siRNA Stability and Reduce Immune Response; 1.5 Cellular Uptake and Intracellular Release of siRNA; 1.6 Combinatorial Targeting for Targeted RNAi Delivery; 1.7 Cell-Specific Aptamer-Functionalized Nanocarriers for RNAi Delivery |
1.8 The Clinical Development and Challenges of siRNAs Therapeutics1.9 Conclusion and Perspectives; References; 2 Analysis of siRNA Delivery Using Various Methodologies; 2.1 Introduction; 2.2 Checkpoints for Analyzing siRNA Delivery; 2.2.1 Circulation Checkpoint; 2.2.2 Organ or Tissue Checkpoint; 2.2.3 Cellular Checkpoint; 2.2.4 RISC Checkpoint; |
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2.2.5 Target mRNA Knockdown (Indirect Checkpoint); 2.2.6 Protein and Outcome (Indirect Checkpoint); 2.2.7 Safety (Indirect Checkpoint); 2.3 Methods for Analysis of siRNA; 2.3.1 General Considerations; 2.3.2 Hybridization-Based (Non-Imaging) Methods |
2.3.3 Non-Hybridization-Based (Non-Imaging) Methods2.3.4 Imaging-Based (Non-Hybridization) Methods; 2.3.5 Imaging-Based (Hybridization) Methods; 2.4 Case Study for siRNA Delivery Analysis; References; 3 Challenges and Opportunities in Bringing RNAi Technologies from Bench to Bed; 3.1 Introduction; 3.2 RNAi Mediator (siRNA or shRNA); 3.2.1 siRNA; 3.2.2 Vector-derived shRNA; 3.2.3 miRNAs; 3.3 Safety Issues of RNAi Mediators; 3.3.1 Immune Stimulation; 3.3.2 RNAi Overexpression; 3.4 Efficacy of RNAi Mediators; 3.4.1 Therapeutic Response; 3.5 RNAi Mediators in Clinical Trials; 3.6 Conclusion |
References4 Nonclinical Safety Assessments and Clinical Pharmacokinetics for Oligonucleotide Therapeutics: A Regulatory Perspective; 4.1 Introduction; 4.2 Unique Properties of Oligonucleotide-based Therapeutics; 4.3 Regulation of Oligonucleotide-Based Therapeutics; 4.3.1 Submission to the FDA; 4.3.2 Review Process for Non-clinical Studies; 4.3.3 Regulatory Issues; 4.3.4 Clinical Pharmacokinetics; 4.4 Conclusion; Disclaimer; Appendix; References; 5 Role of Promoters and MicroRNA Backbone for Efficient Gene Silencing; 5.1 Introduction; 5.2 Promoters for shRNA Expression |
5.2.1 Constitutive Promoters5.2.2 Inducible Promoters; 5.2.3 Site Specific Promoters; 5.3 miRNA-based shRNAs; 5.3.1 miRNA-based shRNA Enhances Gene Silencing; 5.3.2 miRNA-based shRNA Reduces Toxicities; 5.3.3 Application of miRNA-based shRNA for Combination Gene Therapy; 5.4 Concluding Remarks; References; Part 2: RNAi Delivery Strategies; 6 Bioconjugation of siRNA for Site-specific Delivery; 6.1 Introduction; 6.2 Conjugation Strategy; 6.2.1 RNA Chemical Modification; 6.2.2 Site of Conjugation; 6.2.3 Conjugation Chemistry; 6.3 Bioconjugates for Site-specific Delivery |
6.3.1 Antibody-siRNA Bioconjugates |
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Sommario/riassunto |
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"Commonly used by researchers to develop technologies for modifying and studying genetic process, RNA interference (RNAi) has many potential uses in medicine, biotechnology, and functional genomics. This book covers all essential aspects involved in the development of RNAi therapeutics, providing detailed guidance on the challenges and opportunities of bringing RNAi technologies from bench to clinic. It explores the design and mechanism of RNAi molecules, delivery strategies, and therapeutic applications in various diseases. Preclinical, regulatory, market, and intellectual aspects of RNAi technologies are also covered"-- |
"All of the essential aspects of developing RNAi therapeutics from bench to clinic presented in a single volume"-- |
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4. |
Record Nr. |
UNINA9910557671703321 |
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Autore |
Naccarato Attilio |
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Titolo |
Advances in Solid-Phase Microextraction |
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Pubbl/distr/stampa |
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Basel, Switzerland, : MDPI - Multidisciplinary Digital Publishing Institute, 2020 |
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Descrizione fisica |
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1 online resource (168 p.) |
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Soggetti |
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Research & information: general |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Sommario/riassunto |
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Analysis imposes substantial challenges, especially when dealing with analytes present at trace levels in complex matrices. Although modern instrumentation has simplified analyses and makes them more reliable, its use is only the last step of the whole analytical process. On the other hand, sample preparation still represents the bottleneck in many analytical methods and often requires the use of extensive protocols before instrumental analysis. Solid-phase microextraction (SPME) is a well-established sample-prep technique for simultaneous extraction and pre-concentration of compounds from a variety of matrices. Given its compliance with the principles of green analytical chemistry, as well as the simplicity, versatility, and availability of different formats, SPME addresses several challenges associated with the traditional sample preparation approaches and allows for a substantial streamlining of the analytical workflow. This book is the reprint of a Special Issue that includes six contributions provided by some of the world's leading research groups in the field and focuses on recent advances in solid-phase microextraction. |
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