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Record Nr. |
UNINA9910815343903321 |
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Titolo |
ABC of rheumatology / / edited by Ade Adebajo, Lisa Dunkley |
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Pubbl/distr/stampa |
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Hoboken, New Jersey ; ; Chichester, England : , : Wiley Blackwell, , 2018 |
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©2018 |
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ISBN |
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1-118-79318-8 |
1-118-79320-X |
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Edizione |
[5th ed.] |
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Descrizione fisica |
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1 online resource (210 pages) : color illustrations, photographs |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references at the end of each chapters and index. |
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2. |
Record Nr. |
UNINA9910693978403321 |
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Titolo |
Celiac Disease : Methods and Protocols / / edited by Michael N. Marsh |
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Pubbl/distr/stampa |
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Totowa, NJ : , : Humana Press : , : Imprint : Humana, , 2000 |
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ISBN |
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9786610820726 |
9781280820724 |
1280820721 |
9781592590827 |
1592590829 |
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Edizione |
[1st ed. 2000.] |
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Descrizione fisica |
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1 online resource (288 p.) |
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Collana |
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Methods in Molecular Medicine, , 1940-6037 ; ; 41 |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di contenuto |
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Celiac Disease -- Genotyping Methodologies -- From Linkage to Genes -- Linkage and the Transmission Disequilibrium Test in Complex Traits -- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats -- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry -- Synthetic Peptide Libraries for T-Cell Epitope Identification -- Characterization of HLA-DQ-Specific Peptide-Binding Motifs -- Studies of Gliadin-Specific T-Cells in Celiac Disease -- Morphometric Analysis of Intestinal Mucosa -- Morphometry of Rectal Mucosa -- Organ Culture of Rectal Mucosa -- In Situ Hybridization -- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease -- Immunohistochemistry in Research and Diagnosis of Celiac Disease -- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease -- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease -- Whole Gut Lavage Fluid Analysis. |
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Sommario/riassunto |
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With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of |
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other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today. |
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