Hydrogen exchange mass spectrometry of proteins : fundamentals, methods, and applications / / edited by David D. Weis

• Record Nr.: UNINA9910813591403321
• Pubbl/distr/stampa: Chichester, [England] : , : Wiley, , 2016; ©2016
• ISBN: 1-118-70373-1; 1-118-70374-X; 1-118-70369-3
• Descrizione fisica: 1 online resource (544 p.)
• Disciplina: 572/.6
• Soggetti: Proteins - Analysis; Mass spectrometry
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references at the end of each chapters.
• Nota di contenuto: Title Page; Table of Contents; List of Contributors; Foreword; Preface; References; A Note about Nomenclature; References; 1 Hydrogen Exchange; 1.1 Isotopic Exchange and the Study of Protein Conformation and Dynamics; 1.2 Amide HX in Unstructured Polypeptides; 1.3 Amide HX in Folded Polypeptides; References; 2 Hydrogen Exchange Mass Spectrometry Experimental Design; 2.1 Application of HX-MS for Protein Dynamics; 2.2 Factors Governing HX; 2.3 HX-MS Workflow; 2.4 Centroids and Data Analysis; References; 3 Data Processing in Bottom-Up Hydrogen Exchange Mass Spectrometry; 3.1 Introduction; 3.2 The Deuterated Isotopic Distribution3.3 Essential Elements of an HX-MS Data Processing Workflow; 3.4 Select Software Packages for Automation of Analysis; 3.5 Ongoing and Future Challenges; References; 4 Method Validation and Standards in Hydrogen Exchange Mass Spectrometry; 4.1 Introduction; 4.2 Rationale for a Reference Measurement System for HX-MS; 4.3 General Metrological Terminology; 4.4 Method Validation; 4.5 Standards: RM; 4.6 Summary: Maintaining Standards and Monitoring Performance; References; 5 Millisecond Hydrogen Exchange; 5.1 Introduction; 5.2 Instrumentation; 5.3 Data Analysis5.4 Applications; 5.5 Conclusions and Outlook; ; References; 6 Proteases for Hydrogen Exchange Mass Spectrometry; 6.1 Introduction; 6.2 The Use of Pepsin in HX-MS; 6.3 The Use of Other Commercially Available Proteases; 6.4 The Use of Other Acidic Proteases After Expression or Extraction; References; 7 Extracting Information from Hydrogen Exchange Mass Spectrometry Data; 7.1 Introduction; 7.2 Basic Concepts in HX Data Analysis; 7.3 Algorithms for Extracting Rate Constants and Protection Factors; 7.4 Protein Dynamics Hidden in the Isotope Distributions; 7.5 Concluding Remarks and Future ProspectsReferences; 8 Gas-Phase Fragmentation of Peptides to Increase the Spatial Resolution of the Hydrogen Exchange Mass Spectrometry Experiment; 8.1 Why Increase the Spatial Resolution in an HX Experiment Using MS/MS?; 8.2 H/D Scrambling in Peptides and How to Avoid It During MS/MS; 8.3 Integrating Gas-Phase Fragmentation Into the Classical Bottom-Up HX-MS Workflow; 8.4 Recent Applications of the Bottom-Up HX-MS/MS Workflow to Pinpoint the HX Properties of Proteins; 8.5 Future Directions; References; 9 Top-Down Hydrogen Exchange Mass Spectrometry; 9.1 The Appeal of the Top-Down Scheme9.2 Top-Down HX-MS of Small Proteins: The Problem of Hydrogen Scrambling; 9.3 Conformer-Specific Characterization of Nonnative Protein States Using Top-Down HX ECD MS; 9.4 Convergence of Top-Down and Classical Schemes of HX-MS: Combination of Proteolytic and Gas-Phase Fragmentation without Chromatographic Separation; 9.5 The Road Ahead: Challenges and Future Directions; Acknowledgments; References; 10 Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function; 10.1 Introduction; 10.2 Mechanism of Histidine Hydrogen Exchange; 10.3 Historical Context