Genome analysis : current procedures and applications / / edited by Maria S. Poptsova

• Record Nr.: UNINA9910813163403321
• Pubbl/distr/stampa: Norfolk, England : , : Caister Academic Press, , [2014]; ©2014
• ISBN: 1-908230-68-1
• Descrizione fisica: 1 online resource (389 p.)
• Disciplina: 572.8633
• Soggetti: Gene mapping
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Contents; Contributors; Preface; 1 Identification of Structural Variation; Introduction; Defining structural variants; Causes of structural variation; Early methods for SV identification; Identification of structural variation from sequencing data; Discussion; Future trends; 2 Methods for RNA Isolation, Characterization and Sequencing (RNA-Seq); A brief history of RNA; Principles of RNA isolation; Methods of RNA sequencing; Using RNA sequencing to define transcriptional landscapes; RNA sequencing to discover RNA modifications; RNA base modifications and epitranscriptomics; Conclusions; 3 Transcriptome Reconstruction and Quantification from RNA Sequencing DataIntroduction; Transcriptome reconstruction; Transcriptome quantification; Future trends; Conclusions; 4 Identification of Small Interfering RNA from Next-generation Sequencing Data; Introduction; Applying sequencing technologies to siRNAs; Experimental designs; Available tools for analysis of siRNA-seq data; Processing of siRNA-seq data; Locus finding; Association of siRNA loci with genomic features; Differential expression in siRNAs; Phased siRNAs; Post-analysis visualization; Target finding and small RNA networks; Discussion5 Motif Discovery and Motif Finding in ChIP-Seq Data; Introduction; ChIP-Seq data: advantages and challenges for sequence analysis bioinformatics; Cooking recipes for motif analysis of ChIP-Seq data; Conclusion: the present and the future of motif analysis for the ; ChIP-Seq technology; 6 Mammalian Enhancer Prediction; Introduction; Transcriptional enhancers; Computational prediction of enhancers; Discussion and conclusions; Future trends; 7 DNA Patterns for Nucleosome Positioning; Nucleosome as the basic unit of chromatin; Role of nucleosome positioning in gene regulation; Different nucleosome sequence patternsEarly history (pre-genomic and genomic era); Post-genomic era and high-throughput data; Positive versus negative, combining and splitting the patterns; Discussion and conclusions; 8 Hypermethylation in Cancer; Introduction: hypermethylation in the context of other epigenetic modifications; Types of DNA methylation; Hypermethylation machineries: the role of DNMTs; Epigenetic factors contribute to tumourigenesis and cancer progression; Biological pathways of frequently methylated genes in cancer; The relevance of high-throughput technologies as accelerating discovery means of epigenetic events in cancerTranslational applications of methylome analyses as a source of cancer biomarkers; Conclusions; Acknowledgements; References; 9 Identification and Analysis of Transposable Elements in Genomic Sequences; Introduction; Classic detection methods for TEs in genome sequences; TEs in the next-generation sequencing data era; What do NGS data bring to TE analyses?; Conclusions; Future trends; 10 The Current State of Metagenomic Analysis; Introduction; Metagenome sequencing; Metagenome sequencing protocols
• Sommario/riassunto: In recent years, there have been tremendous achievements made in DNA sequencing technologies and corresponding innovations in data analysis and bioinformatics that have revolutionized the field of genome analysis. In this book, an impressive array of experts highlight and review current advances in genome analysis. The book provides an invaluable, up-to-date, and comprehensive overview of the methods currently employed for next-generation sequencing (NGS) data analysis. It also highlights their problems and limitations, and it demonstrates the applications and indicates the developing trends i

Cinchona alkaloids in synthesis and catalysis : ligands, immobilization and organocatalysis / / edited by Choong Eui Song

• Record Nr.: UNINA9911020371203321
• Pubbl/distr/stampa: Weinheim, : Wiley-VCH, c2009
• ISBN: 9786612302510; 9781282302518; 1282302515; 9783527628179; 3527628177; 9783527628186; 3527628185
• Descrizione fisica: 1 online resource (547 p.)
• Classificazione: VK 5500; VK 8620
• Altri autori (Persone): SongChoong Eui
• Disciplina: 547.215; 547.7
• Soggetti: Cinchona alkaloids; Chirality; Organic compounds - Synthesis; Catalysis
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Cinchona Alkaloids in Synthesis and Catalysis: Ligands, Immobilization and Organocatalysis; Contents; Preface; Biography; List of Contributors; 1 An Overview of Cinchona Alkaloids in Chemistry; 1.1 Brief History; 1.2 Active Sites in Cinchona Alkaloids and Their Derivatives; 1.3 Structural Information on Cinchona Alkaloids; 1.4 How This Book Is Organized; References; Part One: Cinchona Alkaloid Derivatives as Chirality Inducers in Metal-Catalyzed Reactions; 2 Cinchona Alkaloids as Chirality Transmitters in Metal-Catalyzed Asymmetric Reductions; 2.1 Introduction; 2.2 Homogeneous Systems for Ketone Reductions2.3 Heterogeneous Pt and Pd Catalysts Modified with Cinchona Alkaloids; 2.3.1 Background; ; 2.3.2 Catalysts; 2.3.3 Modifiers and Solvents; 2.3.4 Substrate Scope for Pt Catalysts; 2.3.4.1 α-Keto Acid Derivatives; 2.3.4.2 α,γ-Diketo Esters; 2.3.4.3 Fluorinated Ketones; 2.3.4.4 α-Keto Acetals; 2.3.4.5 α-Keto Ethers; 2.3.4.6 Miscellaneous Ketones; 2.3.5 Substrate Scope for Pd Catalysts; 2.4 Industrial Applications; 2.5 Conclusions; References; 3 Cinchona Alkaloids as Chiral Ligands in Asymmetric Oxidations; 3.1 Introduction; 3.2 Asymmetric Dihydroxylation of Alkenes3.2.1 Early Reactions; 3.2.2 Bisalkaloid Ligands; 3.2.3 Mechanism; 3.2.4 Variations; 3.2.5 Substrates and Selectivity; 3.2.5.1 Simple Alkenes; 3.2.5.2 Functionalized Alkenes; 3.2.5.3 Polyenes; 3.2.5.3.1 Nonconjugate Olefins; 3.2.5.3.2 Conjugated Polyenes; 3.2.5.4 Double Asymmetric Induction; 3.2.5.5 Resolutions; 3.2.6 Some Reactions of 1,2-Diols; 3.2.6.1 Cyclic Sulfates and Sulfites; 3.3 Aminohydroxylation; 3.4 Sulfur Oxidations; 3.5 Summary; References; 4 Cinchona Alkaloids and their Derivatives as Chirality Inducers in Metal-Promoted Enantioselective Carbon-Carbon and Carbon-Heteroatom Bond Forming Reactions4.1 Introduction; 4.2 Nucleophilic Addition to Carbonyl or Imine Compounds; 4.2.1 Organozinc Addition; 4.2.1.1 Dialkylzinc Addition to Aldehydes; 4.2.1.2 Dialkylzinc Addition to Imines; 4.2.1.3 Addition of Alkynylzincs to Carbonyls; 4.2.2 Asymmetric Reformatsky Reaction; 4.2.3 Indium-Mediated Addition; 4.2.4 Asymmetric Cyanation; 4.2.4.1 Cyanohydrin Synthesis; 4.2.4.2 Strecker Synthesis; 4.2.5 Reactions of Chiral Ammonium Ketene Enolates as Nucleophiles with Different Electrophiles4.2.5.1 Lewis Acid Assisted Nucleophilic Addition of Ketenes (or Sulfenes) to Aldehydes: β-Lactone and β-Sultone Synthesis; 4.2.5.2 Lewis Acid Assisted Nucleophilic Addition of Ketenes to Imines: β-Lactam Synthesis; 4.2.5.3 Applications of Chiral Ketene Enolates to Formal [4 + 2] type Cyclization; 4.2.6 Aza-Henry Reaction; 4.2.7 Enantioselective Hydrophosphonylation; 4.3 Miscellaneous Reactions; 4.3.1 Claisen Rearrangements; 4.3.2 Pd-Catalyzed Asymmetric Allylic Substitutions; 4.3.3 Pauson-Khand Reaction
• Sommario/riassunto: This comprehensive review of cinchona-based chiralilty inducers and their applications covers every topic, including ligands, immobilization and organocatalysis. Each chapter summarizes the scope and limitations of the new methods and technologies, while the final chapter contains carefully selected working procedures of cinchona alkaloid-promoted reactions organized according to reaction type. Invaluable reading for anyone wanting to learn about the current state of this hot topic.