| |
|
|
|
|
|
|
|
|
1. |
Record Nr. |
UNINA9910811730503321 |
|
|
Titolo |
New developments in chromatin research [[electronic resource] /] / Neil M. Simpson and Valerie J. Stewart, editors |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
New York, : Nova Science Publishers, 2012 |
|
|
|
|
|
|
|
ISBN |
|
|
|
|
|
|
Edizione |
[1st ed.] |
|
|
|
|
|
Descrizione fisica |
|
1 online resource (249 p.) |
|
|
|
|
|
|
Collana |
|
Genetics--research and issues |
Cell biology research progress |
|
|
|
|
|
|
|
|
Altri autori (Persone) |
|
SimpsonNeil M |
StewartValerie J |
|
|
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
Soggetti |
|
Chromatin - Research - Methodology |
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Note generali |
|
|
|
|
|
|
Nota di bibliografia |
|
Includes bibliographical references and index. |
|
|
|
|
|
|
Nota di contenuto |
|
Intro -- Library of Congress Cataloging-in-Publication Data -- Contents -- Preface -- The Varied Functions of Aurora Kinases A and B in Mitosis and Carcinogenesis -- Abstract -- Introduction -- Aurora A Kinase -- Aurora A Localization -- Regulation of Aurora A Protein Levels -- Aurora A Mitotic Functions -- Aurora A Functions in Mitotic Entry and Centrosomal Regulation -- Aurora A Functions in Spindle Assembly -- Regulation of Aurora A Activity -- Aurora A and Cancer -- Aurora B Kinase -- Aurora B Localization -- Regulation of Aurora B Levels -- Regulation of Aurora B Activity -- Aurora B Mitotic Functions -- Aurora B Functions in Chromosome Structure and Alignment -- Aurora B Functions in Chromosome Segregation and Anaphase Onset -- Aurora B Functions in Cytokinesis -- Aurora B and Cancer -- Perspectives - Is there a Signaling Cross-Talk between Aurora A and Aurora B? -- Acknowledgments -- References -- Chromatin Structure and Epigenetics -- Abstract -- 1. Introduction -- 2. Chromatin Structure and Nucleosome Dynamics -- 2.1. DNA Structure and Chromatin Fibers -- 2.2. Nucleosome Positioning -- 2.3. Nucleosome Dynamics and Gene Regulation -- 3. Histone Post-Translational Modifications and Chromatin Structure -- 3.1. Types of Histone Post-Translational Modifications -- 3.1.1. Histone Acetylation -- 3.1.2. Histone Methylation -- 3.1.3. Other Modifications -- 3.2. Crosstalk between Histone Post-Translational Modifications -- 3.3. Distribution |
|
|
|
|
|
|
|
|
|
of Histone PTMs within Genomic Domains -- 4. DNA Modifications and Chromatin Structure -- 4.1. DNA Methylation and Demethylation -- 4.1.1. DNA Methylation -- 4.1.2. DNA Demethylation -- 4.2. Crosstalk between DNA Marks and Histone PTMs -- 4.3. MBP Function as Chromatin Architectural Proteins -- Conclusion -- Acknowledgements -- Grant Information -- References. |
Sperm Chromatin Integrity, DNA Fragmentation and Male Fertility -- Abstract -- 1. Sperm Chromatin Structure -- Balhorn's Model -- Toroids -- Histone Bound DNA -- Chromatin Remodelling during Spermatogenesis -- Chromosome Territories -- 2. Sperm Chromatin Integrity, DNA Fragmentation and Male Fertility -- Origin of Sperm DNA Fragmentation -- Incomplete Repair of DNA Breaks during Chromatin Remodelling -- Aberrant Protamination -- Defective Apoptosis -- Excessive Production of ROS in the Ejaculate -- Other Factors -- Methods of Evaluation of Sperm DNA Fragmentation -- Sperm Chromatin Structure Assay (SCSA) -- Terminal Deoxytransferase Mediated deoxyUridine Triphosphate Nick End Labelling (TUNEL) -- Comet Assay -- Sperm Chromatin Dispersion Test -- Impact of Sperm Nuclear Integrity on Male Fertility -- Impact of Sperm Nuclear Integrity on Reproduction -- Natural Conception -- ART Conception -- 3. Future Perspectives -- Fundamental Research Is Essential -- Standardization of the Methods for Sperm DNA Damage Evaluation -- Questions Concerning Factors Influencing the Predictive Value of SDF -- Long-Term Follow Up of ART Children -- References -- The Chromatin State of Pluripotent Stem Cells -- Abstract -- Introduction -- Regulation of Pluripotency in ESCs -- Higher-order Chromatin Architecture in ESCs -- Chromatin Structure in ESCs -- Chromatin Bivalency in ESCs -- ATP-Dependent Chromatin Remodeling -- Histone Variants and Exchange of Histones -- Role of Chromatin in iPSC Generation -- Epigenetic Regulation during iPSC Generation -- Conclusion -- References -- MITF Meets Chromatin in Melanoma -- Abstract -- Introduction -- MITF and Melanoma -- The SWI/SNF Complex -- SWI/SNF in Differentiation and Cancer -- MITF and SWI/SNF in Melanoma -- Conclusion -- Acknowledgments -- Conflict of Interest -- References. |
The State of Chromatin as an Integrative Indicator of Cell Stress -- References -- A Genomics Approach to Analysing DNA Damage and Its Repair throughout Entire Genomes -- Abstract -- Introduction -- Materials and Methods -- UV Irradiation -- DNA Preparation -- Fragmenting DNA by Sonication -- Immunoprecipitation (IP) -- Repair of DNA Damages Prior to PCR Amplification -- PCR Amplification -- Labelling -- Hybridization -- Feature Extraction -- Results -- Detecting CPDs Throughout the Entire Yeast Genome -- Repair of CPDs in Wild Type and rad4 Cells -- Discussion -- Supplementary Data -- Funding -- References -- Recent Patent on Revolver-2: A Novel Transposon-Like Gene Useful for Chromosome Tags of Rye -- Abstract -- Large Gaps of DNA Markers in the Rye Genome -- Rye Genome Specific Transposon-Like -- Gene-Family Revolver -- Highly Quantitative and Structural -- Diversity of Revolver -- Chromosome Tags by Using Revolver -- Current and Future Development -- Acknowledgments -- References -- The Removal of DNA Damage Is Promoted by the Yeast Global Genome Nucleotide Excision Repair Factor Rad16 -- Abstract -- Introduction -- Materials and Methods -- Plasmids and Yeast Strains -- UV Survival Assays -- Chromatin Immunoprecipitation (CHIP) -- Chromatin Preparation, Mnase Digestion, and the High-Resolution Nucleosome Mapping -- Restriction Enzyme Accessibility -- Preparation of Radioactive Probes for Southern Blot Analysis -- UV Treatment of Yeast Cells, DNA Isolation and High Resolution Mapping of CPD Sites -- Results -- UV Induced Histone H3 |
|
|
|
|
|
|
|
|
Acetylation (K9, K14) Requires Both Rad7 and Rad16 -- Rad7 and Rad16 Control H3Ac Status by Regulating Gcn5 Occupancy at MFA2 -- Histone H3 Acetylation Regulates Chromatin Structure at the Promoter of MFA2 -- Increased Histone H3 Acetylation Levels at MFA2 in TUP1 Deleted ( Cells Is Dependent on Gcn5 and Rad16. |
Increased Chromatin Accessibility at MFA2 in TUP1 Deleted ( Cells Depends on Rad16 and Gcn5 -- Increased Histone H3 Acetylation Levels and Open Chromatin Structure are Required for Rad7 and Rad16 Independent GG-NER -- The Atpase and RING Domains of Rad16 Both Contribute to Efficient UV Survival -- The Atpase and RING Domains of Rad16 Are Both Required for UV Induced Gcn5 Occupancy and Histone H3 Acetylation -- The Atpase and RING Domains of Rad16 Are Both Required for Efficient GG-NER -- Discussion -- Acknowledgments -- References -- Chromatin Condensation in Infertile Sperm -- Abstract -- Introduction -- Chromatin Condensation during Spermatogenesis -- DNA Packaging in Mammalian Male Germ Cells -- Human Male Infertility -- Etiology of Sperm DNA Damage -- Protamine Deficiency -- Apoptosis -- Drugs, Chemotherapy, and Radiotherapy -- Reactive Oxygen Species -- Cigarette Smoking -- Post-Testicular Factors -- Varicoceles -- Mechanisms of Sperm DNA Damage -- Evaluation of Sperm DNA Damage -- The Comet Assay -- Terminal dUTP Nick-End Labeling (TUNEL) Assay -- Sperm Chromatin Structure Assay (SCSA) -- Toluidine Blue Staining -- Chromomycin A3 (CMA3) Assay -- Ultrastructure of Spermatozoa from Infertile Men -- Clinical Significance of Sperm DNA Damage -- Conclusion -- References -- Glucocorticoid-Induced Chromatin Remodeling: A Novel Molecular Mechanism of Traumatic Stress -- Abstract -- Introduction -- Chromatin Remodeling -- Chromatin Remodeling, p11 and PTSD -- Chromatin, Memory and Depression -- Conclusion -- References -- Index. |
|
|
|
|
|
| |