Micro- and nanosystems for biotechnology / / edited by J. Christopher Love

• Record Nr.: UNINA9910810800403321
• Pubbl/distr/stampa: Weinheim, Germany : , : Wiley-VCH Verlag GmBH & Company KGaA, , [2016]; ©2016
• ISBN: 3-527-80132-4; 3-527-80129-4
• Descrizione fisica: 1 online resource (305 p.)
• Collana: Advanced biotechnology ; ; volume 2
• Soggetti: Biotechnology; Nanotechnology; Microtechnology
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Cover; Title Page; Copyright; Contents; List of Contributors; About the Series Editors; Preface; Part I Microsystems for Single-Cell Analysis; Chapter 1 Types of Clinical Samples and Cellular Enrichment Strategies; 1.1 Introduction; 1.2 Types of Clinical Samples; 1.2.1 Solid Clinical Samples; 1.2.1.1 Cellular Subtypes Found in Solid Clinical Samples; 1.2.2 Liquid Clinical Samples and Cellular Subtypes; 1.2.2.1 Blood; 1.2.2.2 Bone Marrow; 1.2.2.3 Placental or Umbilical Cord Blood; 1.2.2.4 Urine; 1.2.2.5 Cerebrospinal Fluid (CSF); 1.2.2.6 Saliva; 1.3 Sample Processing and Conventional Methods of Cell Enrichment1.3.1 Processing Solid Clinical Samples; 1.3.1.1 Processing Liquid Samples; 1.3.2 Cell Enrichment; 1.3.2.1 Laser Capture Microdissection (LCM); 1.3.2.2 Density Gradient Centrifugation; 1.3.2.3 Fluorescence-Activated Cell Sorting (FACS); 1.3.2.4 Magnetic Activated Cell Sorting (MACS); 1.3.2.5 CellSearchTM; 1.4 Microscale/Nanoscale Devices for Cellular Enrichment; 1.4.1 Filtration Approaches; 1.4.2 Hydrodynamic Mechanisms; 1.4.3 Surface Treatments; 1.4.4 Magnetophoresis; 1.4.5 Electrophoresis; 1.4.6 Acoustophoresis; 1.4.7 Optical Tweezers/Traps1.5 Conclusion; References; Chapter 2 Genome-Wide Analysis of Single Cells and the Role of Microfluidics; 2.1 ; Motivation for Single-Cell Analysis of Genomes and Transcriptomes; 2.2 Single-Cell Genomics; 2.2.1 Major Technical Challenges; 2.2.2 Approaches to Single-Cell Genomics; 2.2.3 The Application and Impact of Microfluidics in Single-Cell Genomics; 2.3 Single-Cell Transcriptomics; 2.3.1 Major Technical Challenges; 2.3.2 Approaches to Single-Cell Transcriptomics; 2.3.3 Application and Impact of Microfluidics in Single-Cell Transcriptomics; 2.4 The Future of Genome-Wide Single-Cell Analysis with Microfluidics2.4.1 Recent Advances in the Scalability of Single-Cell Analysis using Microfluidics; 2.4.2 How Microfluidics will Expand the Application-Space for Single-Cell Analysis; 2.4.3 Outstanding Hurdles for Genome-Wide Analysis of Single Cells; 2.4.4 Prospects for Clinical Applications of Microfluidic Single-Cell Analysis; Keywords and Definitions; References; Chapter 3 Cellular Immunophenotyping: Industrial Technologies and Emerging Tools; 3.1 Cellular Immune Status and Immunophenotyping; 3.2 Surface Marker Phenotyping; 3.2.1 Multicolor Flow Cytometry3.2.2 Commercial Flow Cytometers; 3.2.3 High-Content Imaging Cytometry; 3.2.4 Current Limitations and Further Development of Flow Cytometry; 3.3 Functional Phenotyping; 3.3.1 ELISpot Technologies; 3.3.2 Multiplexed Immunoassays; 3.3.3 Emerging Single-Cell Technologies; 3.4 Conclusion; Keywords and Definitions; References; Chapter 4 Microsystem Assays for Studying the Interactions between Single Cells; 4.1 Introduction; 4.2 Advantages of Single-Cell Analysis over Conventional Assay Systems; 4.3 Analysis of Cell-Cell Communication between Pairs of Single Cells; 4.3.1 Integrated Microfluidic Coculture Systems and Microwell Arrays