Live cell assays : from research to health and regulatory applications / / Christophe Furger

• Record Nr.: UNINA9910807306403321
• Autore: Furger Christophe
• Pubbl/distr/stampa: Hoboken, New Jersey : , : ISTE Ltd/John Wiley and Sons Inc, , 2016
• ISBN: 1-119-33016-5; 1-119-33017-3; 1-119-33014-9
• Descrizione fisica: 1 online resource (285 p.)
• Collana: Biomedical engineering series
• Disciplina: 571.6
• Soggetti: Cells; Biological assay
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di contenuto: Cover; Title Page; Copyright; Contents; Foreword; A researcher's view; A consultant's view; The author; Acknowledgments; Abbreviations; List of Cell Assays; Introduction; 1: Principles and Position; 1.1. Live cell assay principles; 1.2. Application areas; 1.3. Positioning; 1.3.1. Definition and typology of cell tests; 1.3.2. The regulatory and industrial dimension; 1.4. Market; 1.5. Competitive advantages; 1.5.1. Cells are live information models; 1.5.2. Development: high throughput; 1.5.3. Development: multiplex analysis; 1.5.4. Development: miniaturization; 1.5.5. Development: molecular engineering1.5.6. Development: standardization; 1.6. Can measurements of cells in culture be extrapolated to effects in the organism?; 1.6.1. Toxicokinetics; 1.6.2. Components of the immune system; 1.6.3. Biotransformation; 1.6.4. The macrocellular environment; 1.7. Limits; 1.7.1. Importance of cellular microenvironment; 1.7.2. Other limits; 2: History and State of the Art; 2.1. Origins of cell culture; 2.1.1. Pioneering studies; 2.1.2. Alexis Carrel; 2.1.3. Were Dr Carrel's cells immortal?; 2.2. The HeLa line and the first applications of cell culture; 2.2.1. A vaccine against poliomyelitis2.2.2. Cells in space; 2.2.3. Cell cloning; 2.3. New cell lines; 2.3.1. The CHO line; 2.3.2. An increasing number of cell lines; 2.4. Cross-contamination; 2.5. Cell lines, an ethical issue; 2.6. The first generation of cell assays (1969-1983); ; 2.6.1. The karyotype test; 2.6.2. The MTT assay; 2.6.3. The NRU test; 2.7. The first target of regulatory assays: genotoxicity (1983-1986); 2.7.1. Ames test (OECD guideline 471); 2.7.2. In vitro mammalian chromosome aberration test (OECD guideline 473); 2.7.3. In vitro mammalian cell gene mutation test (OECD guideline 476)2.7.4. In vitro sister chromatid exchange assay in mammalian cells (OECD guideline no. 479); 2.7.5. DNA damage and repair, unscheduled DNA synthesis in mammalian cells (OECD guideline 482); 3: Cell Models and Technologies; 3.1. Fluorescence and bioluminescence; 3.1.1. Green fluorescent protein; 3.1.2. BRET; 3.1.3. FRET; 3.1.4. Other applications of GFP; 3.1.5. The reporter gene approach; 3.2. Impedance variation in cell population; 3.3. Optical signals modified by state of cells; 3.4. Cellular autofluorescence; 3.4.1. The case of chlorophyll3.5. The different cell models and culture modes available; 3.5.1. Immortalized lines; 3.5.2. Primary cells; 3.5.3. Three-dimensional cell culture; 4: Loss of Cell Homeostasis: Applications in Toxicity Measurement; 4.1. What relevant information to use in the living cell?; 4.2. Lysosomal activity; 4.3. Redox balance and oxidative stress; 4.4. Integrity of the plasma membrane; 4.5. Cellular efflux; 4.6. Homeostasis of ion exchanges; 4.6.1. The calcium ion; 4.6.2. Maintenance of membrane potential; 4.7. Metabolism and cell respiratory activity; 4.8. Genotoxicity; 4.9. Apoptosis