Chapter 4: Metabolic Engineering for the Biosynthesis of Terpenoids from Microbial Cell Factories -- 4.1 Introduction -- 4.2 Biosynthesis of Terpenoid -- 4.3 Metabolic Engineering for Microbial Terpenoid Production -- 4.3.1 Metabolic Engineering Strategies for Optimization of Upper Terpenoid Module -- 4.3.1.1 Expressing Heterologous MVA Pathway -- Enzyme Modification -- Utilization of MVA Pathway Enzymes from Other Sources -- Utilization of Synthetic Scaffold -- 4.3.1.2 Tuning Endogenous DXP Pathway -- 4.3.1.3 MVA-Dependent Novel IPP-Bypass Route -- 4.3.2 Metabolic Engineering of Downstream Terpenoid Pathway in Microbes -- 4.3.2.1 Pathway Engineering for Hemiterpenoid Production -- 4.3.2.2 Pathway Engineering for Monoterpenoid Production -- 4.3.2.3 Pathway Engineering for Sesquiterpenoid Production -- 4.3.2.4 Pathway Engineering for Higher Terpenoid Production -- 4.4 Conclusion -- References -- Chapter 5: Modern Analytical Techniques for Extraction, Purification, and Structural Characterization of Microbial Bioactive C... -- 5.1 Introduction -- 5.2 Biomass Processing -- Extraction, Purification, and Characterization of Bioactive Molecules -- 5.2.1 Biomass Propagation -- 5.2.2 Solvent Extraction -- 5.2.3 Chromatographic Purification -- 5.2.4 Structural Characterization of Bioactive Molecules -- 5.2.5 Challenges and Future Scope -- 5.3 Conclusion -- References -- Chapter 6: Application of Alternative Technologies for the Recovery of Bioactive Compounds from Microbial Sources -- 6.1 Introduction -- 6.2 Bioactive Compounds from Microbial Sources -- 6.2.1 Main Microbial Sources of Bioactive Compounds -- 6.2.1.1 Archea Bioactive Compounds -- 6.2.1.2 Bacteria Bioactive Compounds -- 6.2.1.3 Fungi and Yeast Bioactive Compounds -- 6.2.1.4 Microalgae Bioactive Compounds -- 6.3 Production of Bioactive Compounds -- 6.3.1 Conventional Extraction Processes. |