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Record Nr. |
UNINA9910798082103321 |
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Autore |
Kelly Marcy A. |
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Titolo |
The fundamentals of scientific research : an introductory laboratory manual / / by Marcy A. Kelly, PhD ; with contributions from Pryce L. Haddix, PhD |
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Pubbl/distr/stampa |
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Hoboken, New Jersey : , : Wiley Blackwell, , [2015] |
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©2015 |
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ISBN |
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1-118-86772-6 |
1-118-86788-2 |
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Descrizione fisica |
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1 online resource (261 p.) |
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Disciplina |
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Soggetti |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Cover; Table of Contents; Title page; Preface; Acknowledgments; About the Companion Website; Introduction; MODULE 1: Working with and Learning About Common Laboratory Techniques and Equipment; Exercise 1A: Using Common Laboratory Tools to Evaluate Measurements Pre-laboratory Thinking Questions; Directions; Exercise 1B: Using Common Laboratory Tools to Evaluate Measurements; Measurements Introduction; Let's Put This to Practice!; Working with Serological Pipettes and Micropipettes; Let's Put This to Practice!; Practice Pipetting Small Volumes |
Exercise 1C: Using Common Laboratory Tools to Evaluate Measurements Post-laboratory Thinking Questions Directions; Exercise 2A: Using Microscopy to Evaluate Cell Size and Complexity Pre-laboratory Thinking Questions; Directions; Exercise 2B: Using Microscopy to Evaluate Cell Size and Complexity; Microscopy Introduction; Let's Put This to Practice!; Procedure: Steps Used When Viewing a Slide; Exercise 2C: Using Microscopy to Evaluate Cell Size and Complexity Post-laboratory Thinking Questions; Directions; Exercise 3A: The Bacterial Growth Curve Pre-laboratory Thinking Questions; Directions |
Exercise 3B: The Bacterial Growth Curve Introduction; Let's Put This to Practice!; Sample Problems for Graphing on Excel; Answer to Excel |
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Sample Problems; Exercise 3C: The Bacterial Growth Curve Post-laboratory Thinking Questions; Directions; MODULE 2: Working with and Learning About Serratia marcescens in the Laboratory; Exercise 4A: Protein Concentration Versus Growth Stage Pre-laboratory Thinking Questions; Directions; Exercise 4B: Protein Concentration Versus Growth Stage; Introduction; Let's Put This to Practice!; Lysis Protocol; Lowry Assay |
Let's Put This to Practice!Exercise 6C: Formal Laboratory Report Describing the Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens; Introduction; General Instructions; Exercise 7A: Biochemistry of Prodigiosin Production Pre-laboratory Thinking Questions; Directions; Exercise 7B: Biochemistry of Prodigiosin Production; Introduction; Let's Put This to Practice!; Exercise 7C:Biochemistry of Prodigiosin Production Post-laboratory Thinking Questions; Directions |
Exercise 8A: The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise Pre-laboratory Thinking Questions |
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Sommario/riassunto |
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The Fundamentals of Scientific Research: An Introductory Laboratory Manual is a laboratory manual geared towards first semester undergraduates enrolled in general biology courses focusing on cell biology. This laboratory curriculum centers on studying a single organism throughout the entire semester - Serratia marcescens, or S. marcescens, a bacterium unique in its production of the red pigment prodigiosin. The manual separates the laboratory course into two separate modules. The first module familiarizes students with the organism and lab equipment by performing growth curves, Lowry |
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