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Record Nr. |
UNINA9910792267203321 |
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Autore |
Frank J (Joachim), <1940-> |
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Titolo |
Three-dimensional electron microscopy of macromolecular assemblies [[electronic resource] ] : visualization of biological molecules in their native state / / Joachim Frank |
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Pubbl/distr/stampa |
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Oxford ; ; New York, : Oxford University Press, 2006 |
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ISBN |
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0-19-029288-1 |
0-19-803438-5 |
1-4294-1521-5 |
0-19-515096-1 |
1-280-84551-1 |
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Edizione |
[2nd ed.] |
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Descrizione fisica |
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1 online resource (427 p.) |
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Disciplina |
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Soggetti |
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Three-dimensional imaging in biology |
Electron microscopy |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references (p. 345-398) and index. |
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Nota di contenuto |
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Contents; CHAPTER 1 Introduction; 1 The Electron Microscope and Biology; 1.1 General Remarks; 1.2 Three-Dimensional Electron Microscopy; 2 Single-Particle Versus Crystallographic Analysis; 3 Crystallography without Crystals; 4 Toward a Unified Approach to Structural Analysis of Macromolecules; 5 Single-Particle Reconstruction, Macromolecular Machines, and Structural Proteomics; 6 The Electron Microscope and the Computer; CHAPTER 2 Electron Microscopy of Macromolecular Assemblies; 1 Principle of the Transmission Electron Microscope; 2 Specimen Preparation Methods; 2.1 Introduction |
2.2 Negative Staining2.3 Glucose Embedment; 2.4 Use of Tannic Acid; 2.5 Ice-Embedded Specimens; 2.6 Hybrid Techniques: Cryo-Negative Staining; 2.7 Labeling with Gold Clusters; 2.8 Support Grids; 3 Principle of Image Formation in the Transmission Electron Microscope; 3.1 Introduction; 3.2 The Weak-Phase Object Approximation; 3.3 The Contrast Transfer Theory; 3.4 Amplitude Contrast; 3.5 Formulation of Bright-Field Image Formation Using Complex Atomic Scattering Amplitudes; 3.6 Optical and Computational Diffraction Analysis-The |
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