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Record Nr. |
UNINA9910146071003321 |
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Autore |
Kinter Michael |
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Titolo |
Protein sequencing and identification using tandem mass spectrometry [[electronic resource] /] / Michael Kinter, Nicholas E. Sherman |
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Pubbl/distr/stampa |
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New York, : Wiley-Interscience, c2000 |
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ISBN |
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1-280-54166-0 |
9786610541669 |
0-471-23188-6 |
0-471-72198-0 |
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Descrizione fisica |
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1 online resource (321 p.) |
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Collana |
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Wiley-Interscience series on mass spectrometry |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Nucleotide sequence |
Proteins |
Mass spectrometry |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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PROTEIN SEQUENCING AND IDENTIFICATION USING TANDEM MASS SPECTROMETRY; CONTENTS; Series Preface; Preface; Chapter 1. An Introduction to Protein Sequencing Using Tandem Mass Spectrometry; 1.1. Introduction; 1.2. References; Chapter 2. The Primary Structure of Proteins and a Historical Overview of Protein Sequencing; 2.1. Protein and Peptide Structure; 2.2. Edman Degradation; 2.2.1. The Edman Reaction; 2.2.2. Incorporation of the Edman Degradation Reaction into Automated Protein Sequenators; 2.2.3. Edman Degradation in Proteomic Research; 2.3. Tandem Mass Spectrometry |
2.3.1. A Brief History of the Application of Mass Spectrometry to Protein Sequencing2.3.2. Sequence Analysis of Peptides Using Electron Ionization Mass Spectrometry; 2.3.3. The Utilization of Fast Atom Bombardment with Tandem Mass Spectrometry to Sequence Peptides; 2.3.4. Internal Sequence Analysis of Proteins Using Electrospray Ionization-Tandem Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization-Time-of- Flight Mass Spectrometry; 2.4. Summary; 2.5. References; Chapter 3. Fundamental Mass Spectrometry; 3.1. An Overview of the Instrumentation; 3.2. Ionization Methods |
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3.2.1. Electrospray Ionization3.2.2. Nanospray and Microspray Ionization; 3.2.3. Matrix-Assisted Laser Desorption/ Ionization; 3.3. Mass Analyzers; 3.3.1. Fundamental Parameters of Mass Analysis; 3.3.2. Quadrupole Mass Filters (3.20); 3.3.3. Ion Trap Mass Analyzers (3.21-3.23); 3.3.4. Time-of-Flight Mass Analyzers (3.5); 3.4. Tandem Mass Spectrometry; 3.4.1. Collisionally Induced Dissociation; 3.4.2. Tandem Mass Spectrometers; 3.4.3. Types of Tandem Mass Spectrometry Experiments; 3.5. Data Systems; 3.6. Summary; 3.7. References |
Chapter 4. Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra4.1. Introduction; 4.2. Peptide Fragmentation Chemistry; 4.2.1. Collisionally Induced Dissociation of Peptide Ions Formed by Electrospray Ionization; 4.2.2. Fragmentation of Protonated Peptide Ions Formed by Matrix-Assisted Laser Desorption/Ionization; 4.3. Interpretation of the Product Ion Spectra of Tryptic Peptides; 4.3.1. Tabulated Values Used in the Interpretation; 4.3.2. A Strategy for the Interpretation of Product Ion Spectra of Tryptic Peptides |
4.3.3. Sample Interpretation Problem Number One4.3.4. Sample Interpretation Problem Number Two; 4.3.5. A Summary of Interpretation Problems One and Two; 4.3.6. Examples of More Difficult Product Ion Spectra That Cannot Be Completely Interpreted; 4.3.7. Interpretation of Product Ion Spectra from Triply Charged Ions; 4.4. Summary; 4.5. References; Chapter 5. Basic Polyacrylamide Gel Electrophoresis; 5.1. Introduction; 5.2. The Principles of Gel Electrophoresis; 5.2.1. Protein Movement and Separation; 5.2.2. Protein Detection |
5.3. The Basic Steps in a Polyacrylamide Gel Electrophoresis Experiment |
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Sommario/riassunto |
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How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman |
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2. |
Record Nr. |
UNINA9910790099603321 |
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Autore |
Meštrović Stjepan G |
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Titolo |
The coming fin de siècle [[electronic resource] ] : an application of Durkheim's sociology to modernity and postmodernism / / Stjepan G. Meštrović |
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Pubbl/distr/stampa |
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London, : Routledge, 2011 |
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ISBN |
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1-135-16291-3 |
1-283-10213-7 |
9786613102133 |
0-203-85826-3 |
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Descrizione fisica |
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1 online resource (257 p.) |
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Collana |
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Routledge library editions. Emile Durkheim ; ; v. 3 |
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Disciplina |
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Soggetti |
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Postmodernism - Social aspects |
Durkheimian school of sociology |
Rationalism |
Irrationalism (Philosophy) |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Reprint. Originally published: London : Routledge, 1991. |
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Nota di bibliografia |
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Includes bibliographical references and indexes. |
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Nota di contenuto |
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THE COMING FIN DE SIÈCLE An application of Durkheim's sociology to modernity andpostmodernism; Copyright; THE COMING FIN DE SIÈCLE An application of Durkheim's sociology to modernity andpostmodernism; Copyright; Contents; Preface and Acknowledgements; Chapter 1 Back to the Future; Chapter 2 Defining Modernity and Postmodernism; Chapter 3 Durkheim's Era: The Cult of Feeling Versus the Cult of Reason; Chapter 4 Simmel and Durkheim as the First Sociologists of Modernity; Chapter 5 Durkheim's Stand on the Fin De Siècle; Chapter 6 The German Roots of Durkheim's Sociology |
Chapter 7 Ethics Based on the Mind Versus the Heart, Ratioality Versus CompassionChapter 8 Medjugorje, the Virgin Mary, and Medernity; Chapter 9 Postmodern Deregulation and Economic Anomie; Chapter 10 Civilization and its Discontents, Again; Chapter 11 Conclusions: The Coming Fin De Siècle and Postmodernism; References; Name index; |
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Sommario/riassunto |
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First Published in 1991, this book attempts to show the relevance of Durkheim's sociology to the debate on modernity and postmodernism. It does so by examining how Durkheim's ideas can be applied to current social issues. The author argues that there are striking parallels between the social context of the 1890s, when Durkheim began to publish in book form, and today. The book will appeal to the readers of sociology, as well as the related disciplines of philosophy, psychology, cultural studies and history. It is also intended for anyone interested in the issues and questions |
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