1.

Record Nr.

UNINA9910789524503321

Autore

Kohlmann Manuel

Titolo

Design of a single well bioreactor-incubator system with sterile nutrient media supply [[electronic resource] /] / Manuel Kohlmann

Pubbl/distr/stampa

Hamburg, : Diplomica Verlag, 2011

ISBN

3-8428-0894-1

Descrizione fisica

1 online resource (103 p.)

Disciplina

660/.6

Soggetti

Bioreactors

Incubators

Culture media (Biology)

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Title from cover.

Nota di bibliografia

Includes bibliographical references.

Nota di contenuto

Design of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply

Sommario/riassunto

HauptbeschreibungPerfused bioreactors have been developed with different designs and with various cell lines such as fibroblasts, osteoblasts and C2C12 myoblasts. All these current systems are custom made and mostly capable for only one cell type. Small seeding spaces and chambers inhibit longer cell studies and the observation of cell interaction. This study presents an advanced perfusion bioreactor which allows cell observation for longer than 5 days on an area of 2.8cm2 with a volume of 17ml. Initial experiments investigated homogeneity of the heat distribution, which is prec