Bioseparations science and engineering / / Roger G. Harrison [and three others]

• Record Nr.: UNINA9910787493903321
• Autore: Harrison Roger G. <1944->
• Pubbl/distr/stampa: New York : , : Oxford University Press, , 2015; ©2015
• ISBN: 0-19-756276-0; 0-19-021374-4; 1-68015-865-1; 9780195391817; 0-19-021373-6
• Edizione: [2nd edition.]
• Descrizione fisica: 1 online resource (577 p.)
• Collana: Topics in Chemical Engineering : A Series of Textbooks and Monographs
• Disciplina: 660/.2842
• Soggetti: Biomolecules - Separation; Biochemical engineering; Separation (Technology); Extraction (Chemistry)
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: This edition previously issued in print: 2015.
• Nota di bibliografia: Includes bibliographical references at the end of each chapters and index.
• Nota di contenuto: Cover; Bioseparations Science and Engineering; Copyright; Dedication; Contents; Preface; 1 Introduction to Bioproducts and Bioseparations; 1.1 Instructional Objectives; 1.2 Broad Classification of Bioproducts; 1.3 Small Biomolecules; 1.3.1 Primary Metabolites; 1.3.2 Secondary Metabolites; 1.3.3 Summary of Small Biomolecules; 1.4 Macromolecules: Proteins; 1.4.1 Primary Structure; 1.4.2 Secondary Structure; 1.4.3 Tertiary Structure; Example 1.1 Effect of a Reducing Agent on Protein Structure and Mobility; 1.4.4 Quaternary Structure; 1.4.5 Prosthetic Groups and Hybrid Molecules; 1.4.6 Functions and Commercial Uses of Proteins1.4.7 Stability of Proteins; 1.4.8 Recombinant Protein Expression; 1.5 Macromolecules: Nucleic Acids and Oligonucleotides; 1.6 Macromolecules: Polysaccharides; 1.7 Particulate Products; 1.8 Introduction to ; Bioseparations: Engineering Analysis; 1.8.1 Stages of Downstream Processing; Example 1.2 Initial Selection of Purification Steps; 1.8.2 Basic Principles of Engineering Analysis; 1.8.3 Process and Product Quality; 1.8.4 Criteria for Process Development; 1.9 The Route to Market; 1.9.1 The Chemical and Applications Range of the Bioproduct; 1.9.2 Documentation of Pharmaceutical Bioproducts1.9.3 GLP and cGMP; 1.9.4 Formulation; 1.10 Summary; Nomenclature; Problems; References; 2 Analytical Methods and Bench Scale Preparative Bioseparations; 2.1 Instructional Objectives; 2.2 Specifications; 2.3 Assay Attributes; 2.3.1 Precision; 2.3.2 Accuracy; 2.3.3 Specificity; 2.3.4 Linearity, Limit of Detection, and Limit of Quantitation; 2.3.5 Range; 2.3.6 Robustness; 2.4 Analysis of Biological Activity; 2.4.1 Animal Model Assays; 2.4.2 Cell-Line-Derived Bioassays; 2.4.3 In vitro Biochemical Assays; Example 2.1 Coupled Enzyme Assay for Alcohol Oxidase2.5 Analysis of Purity; 2.5.1 Electrophoretic Analysis; Example 2.2 Estimation of the Maximum Temperature in an Electrophoresis Gel; 2.5.2 High-Performance Liquid Chromatography (HPLC); 2.5.3 Mass Spectrometry; 2.5.4 Coupling of HPLC with Mass Spectrometry; 2.5.5 Ultraviolet Absorbance; Example 2.3 Determination of Molar Absorptivity; 2.5.6 CHNO/Amino Acid Analysis (AAA); Example 2.4 Calculations Based on CHNO Analysis; 2.5.7 Protein Assays; 2.5.8 Enzyme-Linked Immunosorbent Assay; 2.5.9 Gas Chromatography; 2.5.10 DNA Hybridization; 2.5.11 ICP/MS (AES)2.5.12 Dry Weight; 2.6 Microbiology Assays; 2.6.1 Sterility; 2.6.2 Bioburden; 2.6.3 Endotoxin; 2.6.4 Virus, Mycoplasma, and Phage; 2.7 Bench Scale Preparative Separations; 2.7.1 Preparative Electrophoresis; 2.7.2 Magnetic Bioseparations; 2.8 Summary; Nomenclature; Problems; References; 3 Cell Lysis and Flocculation; 3.1 Instructional Objectives; 3.2 Some Elements of Cell Structure; 3.2.1 Prokaryotic Cells; 3.2.2 Eukaryotic Cells; 3.3 Cell Lysis; 3.3.1 Osmotic and Chemical Cell Lysis; 3.3.2 Mechanical Methods of Lysis; 3.4 Flocculation; 3.4.1 The Electric Double Layer; Example 3.1 Dependence of the Debye Radius on the Type of Electrolyte
• Sommario/riassunto: An updated edition of a comprehensive and authoritative chemical engineering textbook on bioseparations science, updated to include new information on topics like moment analysis, chromatography, and evaporation.