Biotechnology [[electronic resource] ] : a laboratory course / / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo

• Record Nr.: UNINA9910786464803321
• Autore: Becker Jeffrey M
• Pubbl/distr/stampa: San Diego, : Academic Press, c1990
• ISBN: 0-323-15913-3; 1-299-19350-1
• Descrizione fisica: 1 online resource (251 p.)
• Altri autori (Persone): CaldwellGuy A; ZachgoEve Ann
• Disciplina: 660.6
• Soggetti: Biotechnology
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Dedication; Table of Contents; Preface; Acknowledgments; Suggested Schedule for Exercises; Record Keeping and Safety Rules; Format of Student Laboratory Records; Ten Dos and Don'ts of Record Keeping; Criteria for Grading the Laboratory Notebook; Safety Rules in the Laboratory; Chapter 1. Measurement of pH; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 2. Use of Micropipettors and Spectrophotometers; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 3. Aseptic Technique: Transferring a CultureIntroduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 4. Establishing a Pure Culture: The Streak Plate; Introduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 5. Preparation of Culture Media; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 6. The Growth Curve; Introduction; Reagents/Supplies; Equipment; Procedure; Data Collection and Results; References; Questions; Chapter 7. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep; Introduction; Reagents/SuppliesEquipment; Procedure; References; Questions; Chapter 8. Purification, Concentration, and Quantitation of DNA; Introduction; PART A PURIFICATION OF PLASMID DNA BY SOLVENT ; EXTRACTION; Reagents/Supplies; Equipment; Procedure; PART Β CONCENTRATION OF PLASMID DNA; Reagents/Supplies; Equipment; Procedure; PART C QUANTITATION OF DNA; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 9. Isolation of Plasmid DNA: The Maxi-Prep; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 10. Restriction Digestion and Agarose Gel ElectrophoresisIntroduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 11. Southern Transfer; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 12. Preparation, Purification, and Hybridization of Probe; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 13. Transformation of Saccharomyces cerevisiae; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 14. Transformation of Escherichia coli by Plasmid DNA; IntroductionReagents/Supplies; Equipment; Procedure; References; Questions; Chapter 15. Protein Assays; Introduction; PART A LOWRY PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; PART Β BRADFORD PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; References; Question; Chapter 16. β-Galaetosidase Assay; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 17. Determination of β-Galactosidase in Permeabilized Yeast Cells; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 18. Assay of β-Galactosidase in Cell Extracts
• Sommario/riassunto: Biotechnology