Multi-modality microscopy [[electronic resource] /] / editors, Hanry Yu ...[et al.]

• Record Nr.: UNINA9910784763503321
• Pubbl/distr/stampa: New Jersey, : World Scientific, c2006
• ISBN: 1-281-91958-6; 9786611919580; 981-277-462-9
• Descrizione fisica: 1 online resource (316 p.)
• Altri autori (Persone): YuHanry
• Disciplina: 681/.413
• Soggetti: Confocal microscopy; Polarizing microscopes; Multiphoton excitation microscopy; Fluorescence microscopy
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Contents ; Preface ; Chapter 1. Basic Principles of Imaging ; 1. Light Microscopy ; 2. Image Acquisition ; 3. Data Correction ; 4. Video Enhanced Contrast ; 5. Image Processing ; 6. Deconvolution ; 7. Three-Dimensional Rendering ; References; Chapter 2. Optics for the Biologist: What You Need to Know to Make Your Light Microscope Work Properly 1. Introduction ; 2. The Problem of Spheres ; 3. 'Tube' Length and Magnification ; 4. What to Do? ; 5. Intermediate Summary ; 6. Detecting Spherical Aberration; 7. How Do You Correct Spherical Aberration? 8. Upon Reflection ; 9. Conclusion ; References ; Chapter 3. Spherical Aberration in Confocal Microscopy ; 1. Introduction ; 2. Aberration Introduced by Refractive Index Mismatch; 3. Compensation by Alteration of Immersion Medium Refractive Index ; 4. Validity of the Debye Approximation in the Presence of Spherical Aberration ; 5. Conclusion ; 6. Acknowledgements ; References; Chapter 4. Deconvolution and Image Quality Control - Valuable Tools in Mutti-Dimensional Light Microscopy 1. Introduction ; 2. Background ; 3. Image Restoration ; 4. Image Quality Control ; 5. Conclusion ; 6. Acknowledgements ; References; Chapter 5. Quantitative Polarised Light Microscopy
• Sommario/riassunto: This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engi