Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason

• Record Nr.: UNINA9910784529303321
• Pubbl/distr/stampa: San Diego, Calif., : Academic Press, c1999
• ISBN: 1-281-05700-2; 9786611057008; 0-08-053177-6
• Edizione: [2nd ed.]
• Descrizione fisica: 1 online resource (697 p.)
• Collana: Biological techniques
• Altri autori (Persone): MasonW. T
• Disciplina: 570/.28
• Soggetti: Fluorescent probes; Fluorescence
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Front Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction; 2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References; Chapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General ; properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls; Chapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport; 6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction; 8.2 Photobleaching
• Sommario/riassunto: The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emergi

The impact of regulation on U.S. manufacturing : spotlight on Department of Labor and Department of Transportation : hearing before the Subcommittee on Regulatory Affairs of the Committee on Government Reform, House of Representatives, One Hundred Ninth Congress, first session, June 28, 2005

• Record Nr.: UNINA9910694069403321
• Descrizione fisica: 1 online resource (iii, 152 p.)
• Soggetti: Manufacturing industries - Safety regulations - United States; Labor laws and legislation - United States; Transportation - Safety regulations - United States; Industrial policy - United States
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia

The Challenge to Change : Reforming Health Care on the Front Line in the United States and the United Kingdom / / Rebecca Kolins Givan

• Record Nr.: UNINA9910525543203321
• Autore: Givan Rebecca Kolins <1975->
• Pubbl/distr/stampa: Cornell University Press, 2016; Ithaca, New York ; ; London, [England] : , : ILR Press, , 2016; ©2016
• ISBN: 9781501706578; 1501706578; 9781501706028; 1501706020
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (217 p.)
• Collana: Culture and politics of health care work
• Disciplina: 362.1/0425
• Soggetti: Health care reform - Great Britain; Health care reform - United States; Electronic books.
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Health care systems in the United States and the United Kingdom: a lifetime of change -- Turbulence in the two systems -- Measuring and rewarding performance: imposing change from above in the United Kingdom -- Regulating the frontline from above: the joint commission and hospital regulation in the United States -- Pushing back from the frontline: staff responses to privatization in the National Health Service -- Building a safety culture from the frontline in the United States -- From the health care workplace to the health care system: learning from the United States and United Kingdom.
• Sommario/riassunto: There is constant pressure on hospitals to improve health care delivery and increase cost effectiveness. New initiatives are the order of the day in the dramatically different health care systems of the United States and Great Britain. Often, as we know all too well, these efforts are not successful. In The Challenge to Change, Rebecca Kolins Givan analyzes the successes and failures of efforts to improve hospitals and explains what factors make it likely that the implementation of reforms will be rewarded by positive transformation in a particular institution's day-to-day operation. Givan's in-depth qualitative case studies of both top-down initiatives and changes first suggested by staff on the front lines of care point clearly to the importance of all hospital workers in effecting change and even influencing national policy.Givan illuminates the critical role of workers, managers, and unions in enabling or constraining changes in policies and procedures and ensuring their implementation. Givan spotlights an Anglo-American model of hospital care and work organization, even while these countries retain their differences in access and payment. Entrenched professional roles, hierarchical workplace organization, and the sometimes-detached view of policymakers all shape the prospects for change in hospitals. Givan provides important examples of how the dedication and imagination of the people who work in hospitals can make all the difference when it comes to providing quality health care even in a challenging economic environment.