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Record Nr. |
UNINA9910782096403321 |
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Titolo |
Biological low-voltage scanning electron microscopy [[electronic resource] /] / edited by Heide Schatten, James B. Pawley |
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Pubbl/distr/stampa |
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New York, : Springer, c2008 |
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ISBN |
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1-281-13990-4 |
9786611139902 |
0-387-72972-0 |
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Edizione |
[1st ed. 2008.] |
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Descrizione fisica |
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1 online resource (328 p.) |
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Altri autori (Persone) |
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SchattenHeide |
PawleyJames B |
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Disciplina |
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Soggetti |
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Low-voltage scanning electron microscopy |
Field emission cathodes |
Scanning electron microscopes |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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The Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy. |
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Sommario/riassunto |
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Field-emission, low-voltage scanning electron microscopy (LVSEM) is a field that has grown tremendously in recent years because is offers the optimal method for viewing complex surfaces at high resolution and in three dimensions. However, even though the instrumentation required to get good results at low beam voltage has become increasingly available, there has been a lag in its application to biological |
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specimens. What seemed to be missing was volume that combined both the theory and practice of using this equipment in an optimal manner with a thorough treatment of biological specimen preparation. Biological Low-Voltage Scanning Electron Microscopy is the first book to address both of these aspects of biological LVSEM. After providing a thorough description of the unique advantages and the operating constraints related to operating a scanning electron microscope at low beam voltage, the remainder of book focuses on the the best way to image all types of plant and animal cells and covers specimens that range from macromolecules to the surfaces revealed by de-embedding resin-embedded samples. Advanced specimen preparation techniques such as cryo-LVSEM, and immuno-gold-LVSEM are fully covered, as is x-ray microanalysis at low beam voltage and live-time stereo imaging. The preparative protocols provided represent the distilled essence of the experience of a group of world-renowned authors who have, for many decades, been instrumental in developing and applying new approaches to LVSEM to support their own biological research. |
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2. |
Record Nr. |
UNINA9910830764803321 |
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Autore |
Gallant Brian |
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Titolo |
Hazardous waste operations and emergency response manual [[electronic resource] /] / Brian Gallant |
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Pubbl/distr/stampa |
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Hoboken, N.J., : Wiley-Interscience, c2006 |
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ISBN |
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1-280-31157-6 |
9786610311576 |
1-61344-896-1 |
0-470-00725-7 |
0-470-00724-9 |
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Descrizione fisica |
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1 online resource (345 p.) |
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Disciplina |
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Soggetti |
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Hazardous wastes - Purification |
Hazardous wastes - Management |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Nota di contenuto |
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HAZARDOUS WASTE OPERATIONS AND EMERGENCY RESPONSE MANUAL; CONTENTS; 1 REGULATIONS, AGENCIES, AND RESOURCES; Introduction-History of Employee Health and Safety Regulations; Regulations; The Environmental Protection Agency; Hazardous Waste Numbers; EPA Identification Numbers; Clean Water Act; Clean Air Act; Resource Conservation and Recovery Act (RCRA); Toxic Substance Control Act; Comprehensive Environmental Response Compensation and Liability Act (CERCLA); Superfund Amendment and Reauthorization Act (SARA); Department of Labor-Occupational Safety and Health Administration |
National Institute for Occupational Safety and Health (NIOSH)Department of Transportation (DOT); National Fire Protection Association (NFPA); NFPA 704 Labeling; Hazard Communication Standard; Hazardous Waste Operations and Emergency Response (HAZWOPER); Hazwoper Training; Incident Command System (ICS); Resources; Material Safety Data Sheets (MSDS); NIOSH Pocket Guide to Chemical Hazards; Emergency Response Guidebook; Summary; 2 HAZARD CLASSIFICATION; Chemical Exposure; Explosion and Fire; Oxygen Deficiency; Ionizing Radiation; Biological Hazards; General Safety Hazards; Electrical Hazards |
Heat StressCold Exposure; Noise; Poisonous Snakes, Insects, and Plants; Weather; Heavy Equipment; Tools; Definition of Hazardous Materials vs. Hazardous Waste; Classification of Hazardous Materials; Physical Properties of Hazardous Materials; Vapor Density and Specific Gravity; Flammability; Explosive Limits; Flash Point; Flammable Solids; Firefighting and Fire Prevention; Portable Fire Extinguishers; Toxic Products of Combustion; Corrosives; Acids; Alkalis; Reactivity of Some Common Elements; Water-Reactive Materials; Oxidizing Materials; Boiling Liquid Expanding Vapor Explosion (BLEVE) |
Flammable and Combustible LiquidSummary; 3 SITE SAFETY PLAN; The Plan; Emergencies; Incident Characterization; Remedial Actions; Safety Plan Development; Routine Operations; Describe the Known Hazards and Risks; List Key Personnel and Alternates; Designate Levels of Protection to be Worn; Delineate Work Areas; List Control Procedures; Establish Decontamination Procedures; Address Requirements for an Environmental Surveillance Program; Specify Any Routine and/or Special Training Required; Establish Procedures for Weather-Related Problems; On Site Emergencies |
Establish Site Emergency ProceduresAddress Emergency Medical Care; Implementation of the Site Safety Plan; Typical Safety Plan Outline; Responsibilities; Client; Engineering Firm; Site Contractors; Consulting Firm / Site Safety Officer (SSO); Summary; 4 SITE CHARACTERIZATION; Offsite Characterization; Interview/Records Research; Perimeter Investigation; Protection of Site Entry Workforce; Onsite Survey; Continuing the Survey; Information Documentation; Hazard Assessment; Threshold Limit Values; Permissible Exposure Limit; Recommended Exposure Limit; IDLH Concentrations |
Potential Skin Absorption and Irritation |
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Sommario/riassunto |
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Field technicians and emergency response personnel are often faced with the dangers of flammable, combustible, and chemically unstable materials. Although there are numerous procedures set forth by regulatory agencies like the Occupational Safety and Health Administration (OSHA), the Environmental Protection Agency (EPA), and the National Institute for Occupational Safety and Health (NIOSH) for effectively and safely dealing with such environmental hazards, up until now there has been no single resource for training in this area.Based on the author's twenty-plus years of field experien |
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