1.

Record Nr.

UNINA9910780717403321

Autore

Lehmann Wolf D

Titolo

Protein phosphorylation analysis by electrospray mass spectrometry [[electronic resource] ] : a guide to concepts and practice / / Wolf D. Lehmann

Pubbl/distr/stampa

Cambridge, : RSC Pub., c2010

ISBN

1-84973-220-5

Descrizione fisica

1 online resource (395 p.)

Disciplina

572.636

Soggetti

Electrospray ionization mass spectrometry

Proteins - Analysis

Phosphorylation

Mass spectrometry

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Acknowledgements -- ; ch. 1. Introduction -- ; ch. 2. Analysis of phosphopeptides by mass spectronomy -- ; ch. 3. Phosphopeptide enrichment -- ; ch. 4. Dephosphorylation -- ; ch. 5. Protein phosphorylation and element mass spectronomy -- ; ch. 6. Structural phosphorylation analysis -- ; ch. 7. Quantitative protein phosphorylation analysis -- Outlook -- Subject index.

Sommario/riassunto

Protein phosphorylation analysis is a central theme in current analytical biochemistry, cell biology and systems biology. Due to its versatility, specificity and sensitivity, mass spectrometry has developed into a key technology in this field. A set of minor and major instrumental innovations mean that mass spectrometers now exhibit a level of performance, a stability of operation, a relative ease of use, and productivity, which would once have been hard to imagine. This book guides the reader through this prolific field by presenting a collection of personal views and selected examples which