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Record Nr. |
UNINA9910779848203321 |
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Titolo |
Topics in fluorescence spectroscopy : principles [[electronic resource] /] / edited by Joseph R. Lakowicz |
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Pubbl/distr/stampa |
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New York, : Kluwer Academic, c2002 |
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ISBN |
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1-280-20633-0 |
9786610206339 |
0-306-47058-6 |
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Edizione |
[1st ed. 2002.] |
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Descrizione fisica |
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1 online resource (449 p.) |
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Collana |
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Topics in fluorescence spectroscopy ; ; v.2 |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Diagnostic imaging |
Fluorescence spectroscopy |
Lasers - Diagnostic use |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Fluorescence Anisotropy: Theory and Applications -- Fluorescence Quenching: Theory and Applications -- Resonance Energy Transfer -- Least-Squares Analysis of Fluorescence Data -- The Global Analysis of Fluorescence Intensity and Anisotropy Decay Data: Second-Generation Theory and Programs -- Fluorescence Polarization from Oriented Systems -- Fluorescence-Based Fiber-Optic Sensors -- Inhomogeneous Broadening of Electronic Spectra of Dye Molecules in Solutions. |
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Sommario/riassunto |
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Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the |
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rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3). |
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