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1. |
Record Nr. |
UNINA9910455575003321 |
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Autore |
Thoreau Henry David <1817-1862.> |
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Titolo |
"Wild apples" and other natural history essays [[electronic resource] /] / by Henry D. Thoreau ; edited by William Rossi |
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Pubbl/distr/stampa |
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Athens, : University of Georgia Press, c2002 |
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ISBN |
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1-282-55288-0 |
9786612552885 |
0-8203-2636-4 |
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Descrizione fisica |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Natural history |
Natural history - Massachusetts |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Bibliographic Level Mode of Issuance: Monograph |
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Nota di bibliografia |
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Includes bibliographical references (p. 221-226) and index. |
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2. |
Record Nr. |
UNINA9910136804003321 |
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Autore |
Feng Gao |
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Titolo |
DNA replication origins in microbial genomes [[electronic resource] /] / edited by Feng Gao |
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Pubbl/distr/stampa |
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Frontiers Media SA, 2016 |
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France : , : Frontiers Media SA, , 2016 |
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ISBN |
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Descrizione fisica |
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1 online resource (115 pages) : illustrations, charts |
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Collana |
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Frontiers Research Topics |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references. |
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Sommario/riassunto |
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DNA replication, a central event for cell proliferation, is the basis of biological inheritance. Complete and accurate DNA replication is integral to the maintenance of the genetic integrity of organisms. In all three domains of life, DNA replication begins at replication origins. In bacteria, replication typically initiates from a single replication origin (oriC), which contains several DnaA boxes and the AT-rich DNA unwinding element (DUE). In eukaryotic genomes, replication initiates from significantly more replication origins, activated simultaneously at a specific time. For eukaryotic organisms, replication origins are best characterized in the unicellular eukaryote budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe. The budding yeast origins contain an essential sequence element called the ARS (autonomously replicating sequence), while the fission yeast origins consist of AT-rich sequences. Within the archaeal domain, the multiple replication origins have been identified by a predict-and-verify approach in the hyperthermophilic archaeon Sulfolobus. The basic structure of replication origins is conserved among archaea, typically including an AT-rich unwinding region flanked by several short repetitive DNA sequences, known as origin recognition boxes (ORBs). It appears that archaea have a simplified version of the eukaryotic replication apparatus, which has led to considerable interest in the |
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archaeal machinery as a model of that in eukaryotes. The research on replication origins is important not only in providing insights into the structure and function of the replication origins but also in understanding the regulatory mechanisms of the initiation step in DNA replication. Therefore, intensive studies have been carried out in the last two decades. The pioneer work to identify bacterial oriCs in silico is the GC-skew analysis. Later, a method of cumulative GC skew without sliding windows was proposed to give better resolution. Meanwhile, an oligomer-skew method was also proposed to predict oriC regions in bacterial genomes. As a unique representation of a DNA sequence, the Z-curve method has been proved to be an accurate and effective approach to predict bacterial and archaeal replication origins. Budding yeast origins have been predicted by Oriscan using similarity to the characterized ones, while the fission yeast origins have been identified initially from AT content calculation. In comparison with the in silico analysis, the experimental methods are time-consuming and labor-intensive, but convincing and reliable. To identify microbial replication origins in vivo or in vitro, a number of experimental methods have been used including construction of replicative oriC plasmids, microarray-based or high-throughput sequencing-based marker frequency analysis, two-dimensional gel electrophoresis analysis and replication initiation point mapping (RIP mapping). The recent genome-wide approaches to identify and characterize replication origin locations have boosted the number of mapped yeast replication origins. In addition, the availability of increasing complete microbial genomes and emerging approaches has created challenges and opportunities for identification of their replication origins in silico, as well as in vivo and in vitro. |
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3. |
Record Nr. |
UNINA9910585944203321 |
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Autore |
Benasciutti Denis |
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Titolo |
Fracture, Fatigue, and Structural Integrity of Metallic Materials and Components Undergoing Random or Variable Amplitude Loadings |
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Pubbl/distr/stampa |
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Basel, : MDPI - Multidisciplinary Digital Publishing Institute, 2022 |
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Descrizione fisica |
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1 online resource (190 p.) |
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Soggetti |
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History of engineering and technology |
Technology: general issues |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Sommario/riassunto |
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Most metallic components and structures are subjected, in service, to random or variable amplitude loadings. There are many examples: vehicles subjected to loadings and vibrations caused by road irregularity and engine, structures exposed to wind, off-shore platforms undergoing wave-loadings, and so on. Just like constant amplitude loadings, random and variable amplitude loadings can make fatigue cracks initiate and propagate, even up to catastrophic failures. Engineers faced with the problem of estimating the structural integrity and the fatigue strength of metallic structures, or their propensity to fracture, usually make use of theoretical, numerical, or experimental approaches. This reprint collects a series of recent scientific contributions aimed at providing an up-to-date overview of approaches and case studies-theoretical, numerical or experimental-on several topics in the field of fracture, fatigue strength, and the structural integrity of metallic components subjected to random or variable amplitude loadings. |
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