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Record Nr. |
UNINA9910555273503321 |
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Autore |
Sanderson J. B (Jeremy B.) |
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Titolo |
Understanding light microscopy / / by Jeremy Sanderson |
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Pubbl/distr/stampa |
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Hoboken, NJ : , : Wiley, , 2019 |
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ISBN |
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1-118-69681-6 |
1-118-69674-3 |
1-118-69673-5 |
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Descrizione fisica |
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1 online resource (850 pages) |
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Collana |
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Royal Microscopical Society/John Wiley Series |
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Disciplina |
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Soggetti |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Our eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied. |
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