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Record Nr. |
UNINA9910133642003321 |
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Autore |
Sauer Markus |
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Titolo |
Handbook of fluorescence spectroscopy and imaging : from single molecules to ensembles / / Markus Sauer, Johan Hofkens, and Jorg Enderlein |
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Pubbl/distr/stampa |
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Weinheim, : Wiley-VCH, 2011 |
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ISBN |
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9786613140630 |
9783527633524 |
3527633529 |
9781283140638 |
1283140632 |
9783527633517 |
3527633510 |
9783527633500 |
3527633502 |
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Descrizione fisica |
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1 online resource (293 p.) |
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Altri autori (Persone) |
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HofkensJohan |
EnderleinJ (Jorg) |
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Disciplina |
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Soggetti |
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Fluorescence spectroscopy |
Fluorescence microscopy |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Handbook of Fluorescence Spectroscopy and Imaging: From Single Molecules to Ensembles; Contents; Preface; 1 Basic Principles of Fluorescence Spectroscopy; 1.1 Absorption and Emission of Light; 1.2 Spectroscopic Transition Strengths; 1.3 Lambert-Beer Law and Absorption Spectroscopy; 1.4 Fluorophore Dimerization and Isosbestic Points; 1.5 Franck-Condon Principle; 1.6 Temperature Effects on Absorption and Emission Spectra; 1.7 Fluorescence and Competing Processes; 1.8 Stokes Shift, Solvent Relaxation, and Solvatochroism; 1.9 Fluorescence Quantum Yield and Lifetime; 1.10 Fluorescence Anisotropy |
References2 Fluorophores and Fluorescent Labels; 2.1 Natural |
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Fluorophores; 2.2 Organic Fluorophores; 2.3 Different Fluorophore Classes; 2.4 Multichromophoric Labels; 2.5 Nanocrystals; References; 3 Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS); 3.1 In Vitro Fluorescence Labeling; 3.2 Fluorescence Labeling in Living Cells; References; 4 Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways; References; 5 Fluorescence Correlation Spectroscopy; 5.1 Introduction; 5.2 Optical Set-Up; 5.3 Data Acquisition and Evaluation |
5.4 Milliseconds to Seconds: Diffusion and Concentration5.4.1 Single-Focus FCS; 5.4.2 Dual-Focus FCS; 5.5 Nanoseconds to Microseconds: Photophysics, Conformational Fluctuations, Binding Dynamics; 5.6 Picoseconds to Nanoseconds: Rotational Diffusion and Fluorescence Antibunching; 5.6.1 Antibunching; 5.6.2 Rotational Diffusion; 5.7 Fluorescence Lifetime Correlation Spectroscopy; 5.8 Conclusion; References; 6 Excited State Energy Transfer; 6.1 Introduction; 6.2 Theory of (F€orster) Energy Transfer; 6.2.1 Mechanism and Mathematical Formalism of FRET |
6.2.2 Measuring FRET Efficiencies Through Excited-State Lifetimes6.2.3 Spin Rules for FRET; 6.2.4 Homo-FRET and FRET-Induced Depolarization; 6.3 Experimental Approach for Single-Pair FRET-Experiments; 6.3.1 Single-Laser Excitation; 6.3.2 Alternating-Laser Excitation (ALEX); 6.4 Examples and Applications of FRET; 6.4.1 FRET Processes in Bulk Experiments; 6.4.1.1 FRET-Based Molecular Biosensors; 6.4.1.2 Energy Hopping and Trapping in Chromophore-Substituted Polyphenylene Dendrimers; 6.4.2 Single-Molecule Observation of FRET; 6.4.2.1 Light-Harvesting Systems: Phycobilisomes and Allophycocyanins |
6.4.2.2 Hairpin Ribozyme Dynamics and Activity6.4.2.3 Protein (Un)folding and Dynamics; References; 7 Photoinduced Electron Transfer (PET) Reactions; 7.1 Fluorescence Quenching by PET; 7.2 Single-Molecule Fluorescence Spectroscopy to Study PET; 7.3 Single-Molecule Sensitive Fluorescence Sensors Based on PET; 7.4 PET Reporter System; 7.5 Monitoring Conformational Dynamics and Protein Folding by PET; 7.6 Biological and Diagnostic Applications; References; 8 Super-Resolution Fluorescence Imaging; 8.1 Diffraction Barrier of Optical Microscopy |
8.2 Multi-Photon and Structured Illumination Microscopy |
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Sommario/riassunto |
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Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging.Examples show how each technique can help in obtaining detailed and refine |
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2. |
Record Nr. |
UNINA9910484730503321 |
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Titolo |
Contaminants in Drinking and Wastewater Sources : Challenges and Reigning Technologies / / edited by Manish Kumar, Daniel D. Snow, Ryo Honda, Santanu Mukherjee |
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Pubbl/distr/stampa |
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Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2021 |
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ISBN |
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Edizione |
[1st ed. 2021.] |
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Descrizione fisica |
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1 online resource (447 pages) : illustrations |
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Collana |
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Springer Transactions in Civil and Environmental Engineering, , 2363-7641 |
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Disciplina |
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Soggetti |
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Pollution |
Water |
Hydrology |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di contenuto |
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Pharmaceuticals, Personal Care Products and Artificial Sweeteners in Asian Groundwater: A Review -- Affinity-Based Methods for the Analysis of Emerging Contaminants in Wastewater and Related Samples -- Natural Attenuation of Pharmaceuticals in the Aqueous Environment and Role of Photodegradation in Surface Water -- Impact and Fate of Microplastic in the Aquatic Environment -- Assessment of Groundwater Quality in Sri Lanka Using Multivariate Statistical Techniques -- Source and Fate of Perchlorate in the Environment: A Grave Concern for World -- Carcinogenic Nature of Emerging Contaminants: Havoc for Present and Gateway of Unhealthy Future -- Permeable Reactive Barrier: A Sustainable Groundwater Remediation Technology -- An Insight into Microbial Remediation of Hexavalent Chromium from Contaminated Water -- Current Understanding on Separation of Personal Care Products and Pharmaceuticals from Water -- Nanotechnology: An Efficient Technique of Contaminated Water Treatment and Bioremediation Methods of Water Contaminants -- Chlorophenols Dechlorination Water Treatment Using Ni-Iron Bimetallic Systems: Implications of the Degree of Chlorination, Nickel Coating, and Iron Oxide Phases. |
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Sommario/riassunto |
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This volume takes a multidisciplinary approach to study and evaluate |
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the global human vulnerability to the exposure of contaminants of emerging concern (CECs) in the natural environment. It provides a comprehensive resource on structurally diverse groups of chemical compounds that have adverse effects on the aquatic environment. It explores the global strength, environmental status, chemical risk assessment and management strategies of CECs with relevant modern techniques. The principle focus is on concurrent emerging water quality issues. It defines the impacts of the environmental exposure of trace concentrations of CECs and/or their metabolites and discusses possible technological advances to combat the emerging pollutants. It will be useful to researchers, multi-stakeholder expert groups, policymakers, and graduate students. |
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