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1. |
Record Nr. |
UNIBAS000041079 |
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Autore |
Alcuinus |
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Titolo |
De orthographia / Alcuino ; edizione critica a cura di Sandra Bruni |
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Pubbl/distr/stampa |
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Firenze : Sismel Edizioni del Galluzzo, 1997 |
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ISBN |
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Descrizione fisica |
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Collana |
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Millennio medievale , Testi ; 2 |
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Disciplina |
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Soggetti |
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Alcuino. De orthographia Critica |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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2. |
Record Nr. |
UNINA9910480517403321 |
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Titolo |
Code-switching - experimental answers to theoretical questions : in honor of Kay Gonzá́́́lez-Vilbazo / / edited by Luis López, The University of Illinois at Chicago |
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Pubbl/distr/stampa |
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Amsterdam ; ; Philadelphia : , : John Benjamins Publishing Company, , [2018] |
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©2018 |
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Descrizione fisica |
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1 online resource (280 pages) |
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Collana |
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Issues in Hispanic and Lusophone linguistics (IHLL), , 2213-3887 ; ; Volume 19 |
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Disciplina |
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Soggetti |
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Code switching (Linguistics) |
Bilingualism |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Collected papers written in honor of Professor Kay González-Vilbazo of |
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The University of Illinois at Chicago. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Theory and methodology in code-switching research / Luis López -- Gender assignment in Basque/Spanish mixed determiner phrases: A study of simultaneous bilinguals / Lucia Badiola and Ariane Sande -- The familiar and the strange: Gender assignment in Spanish/English mixed DPs / Rodrigo Delgado -- Adjective placement in Spanish and Basque mixed DPs / Irati de Nicolás and Jon Robledo -- That-trace effects in Spanish-English code-switching / Shane Ebert and Bradley Hoot -- Modality in experimental code-switching research: aural versus written stimuli / Bryan Koronkiewicz and Shane Ebert -- Event-related potentials reveal evidence for syntactic co-activation in bilingual language processing: A replication of Sanoudaki and Thierry (2014, 2015) / Alicia Luque, Nethaum Mizyed and Kara Morgan-Short -- Phonological factors of Spanish/English word internal code-switching / Sara Stefanich and Jennifer Cabrelli Amaro -- Basque complementizers under the microscope: A Spanish/Basque code-switching approach / Daniel Vergara -- The future of code-switching research / Almeida Jacqueline Toribio. |
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3. |
Record Nr. |
UNINA9910253889603321 |
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Titolo |
Advanced Technologies for Protein Complex Production and Characterization / / edited by M. Cristina Vega |
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Pubbl/distr/stampa |
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016 |
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ISBN |
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Edizione |
[1st ed. 2016.] |
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Descrizione fisica |
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1 online resource (XIII, 382 p. 87 illus., 71 illus. in color.) |
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Collana |
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Advances in Experimental Medicine and Biology, , 2214-8019 ; ; 896 |
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Disciplina |
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Soggetti |
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Proteins |
Bioinformatics |
Protein Biochemistry |
Computational and Systems Biology |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Protein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants—potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. . |
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Sommario/riassunto |
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This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies. |
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