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1. |
Record Nr. |
UNINA9910466449803321 |
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Autore |
Solís Mireya |
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Titolo |
Dilemmas of a trading nation : the United States and Japan in the evolving Trans-Pacific order / / Mireya Solis |
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Pubbl/distr/stampa |
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Washington, DC : , : Brookings Institution Press, , 2017 |
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©2017 |
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Edizione |
[1st Edition.] |
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Descrizione fisica |
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1 online resource (305 pages) : illustrations |
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Collana |
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Geopolitics in the 21st Century |
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Disciplina |
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Soggetti |
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Electronic books. |
Japan Commercial policy |
United States Commercial policy |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Sommario/riassunto |
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"Japan is at a critical moment in determining its trade policy as it strives for renewed economic growth. Its economy still struggling after two decades of low growth, Japan now faces a difficult moment as it confronts this ongoing challenge to economic renewal. Tokyo could deploy a proactive trade policy to help it rise again as one of the world's greatest trading nations. It could also, at the same time, attack the structural problems that have hindered its economic competitiveness and kept it from becoming a leading voice in the drafting of rules for this century's global economy. Or, it could do nothing and remain shackled to the domestic political constraints that have kept it from playing a central role in international trade negotiations. In Dilemmas of a Trading Nation, Mireya Solis describes how Japan's economic choices are important for the United States, as well. The two nations are the most important members of the Trans-Pacific Partnership (TPP), the trade agreement concluded in 2015 intended to spur trade in the world's fastest-growing economic region. The arrest of Japan's economic decline, the credibility of America's resolve to remain a Pacific power, and the deepening of the bilateral alliance are all influenced significantly by the outcome of the TPP |
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agreement. But the domestic politics of trade policy have never been as unwieldy as policymakers across the Pacific aim to negotiate ever more ambitious trade and to marshal domestic support for them. Dilemmas of a Trading Nation describes how, for both Japan and the United States, the stakes involved in addressing the tradeoffs of trade policy design could not be higher"-- |
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2. |
Record Nr. |
UNINA9910957092703321 |
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Autore |
Carson Sue (Susan) |
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Titolo |
Molecular biology techniques : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
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Pubbl/distr/stampa |
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Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
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ISBN |
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9786613310972 |
9781283310970 |
128331097X |
9780123855459 |
0123855454 |
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Edizione |
[3rd ed.] |
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Descrizione fisica |
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1 online resource (xxvi, 200 pages) : color illustrations |
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Altri autori (Persone) |
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MillerHeather B |
WitherowD. Scott |
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Disciplina |
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Soggetti |
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Molecular biology |
Molecular biology - Technique |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Includes index. |
Previous edition: 2006. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 |
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Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
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Sommario/riassunto |
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, |
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