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Record Nr. |
UNINA9910463255903321 |
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Titolo |
PCR technology : current innovations / / edited by Tania Nolan, Stephen A. Bustin |
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Pubbl/distr/stampa |
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Boca Raton : , : Taylor & Francis, , 2013 |
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ISBN |
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0-429-11045-6 |
1-4398-4813-0 |
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Edizione |
[3rd ed.] |
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Descrizione fisica |
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1 online resource (457 p.) |
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Altri autori (Persone) |
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NolanTania |
BustinStephen A. <1954-> |
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Disciplina |
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Soggetti |
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DNA polymerases |
Transferases |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references. |
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Nota di contenuto |
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pt. 1. Template preparation -- pt. 2. Reaction components -- pt. 3. Instruments -- pt. 4. Design, optimization, QC, and standardization -- pt. 5. Data analysis -- pt. 6. Applications. |
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Sommario/riassunto |
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A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution--Provided by publisher. |
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