1.

Record Nr.

UNINA9910460492703321

Titolo

American Studies as Transnational Practice : Turning toward the Transpacific / / edited by Yuan Shu and Donald E. Pease

Pubbl/distr/stampa

Hanover, New Hampshire : , : Dartmouth College Press, , [2015]

©[2015]

ISBN

1-61168-848-5

Descrizione fisica

1 online resource (418 p.)

Collana

Re-mapping the transnational : a Dartmouth series in American studies.

Disciplina

810.9/895

Soggetti

Transnationalism - United States

Culture in literature

Imperialism in literature

Transnationalism in literature

Literature and transnationalism - United States

American literature - Asian American authors - History and criticism

Electronic books.

United States Study and teaching

Asia In literature

Pacific Area In literature

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Includes index.

Nota di bibliografia

Includes bibliographical references (pages 384-388) and index.

Nota di contenuto

Cover; Title Page; Contents; Introduction: Transnational American Studies and the Transpacific Imaginary; Part 1 | Transnational Practices: Outside/Inside American Studies; 1 | How Transnationalism Reconfigured the Field of American Studies: The Transnational/Dispora Complex; 2 | Post-Soviet American Studies; 3 | Transnationalism, Planetary Consciousness, and American Studies; Part 2 | Deep Maps, Postracial Imaginaries, Diasporized Networks, and Other Transnational Literary Assemblages; 4 | Transnational Mark Twain; 5 | Racial Memory and the Modern Borders of the Nation-State

6 | The Other Side of History, The Other Side of Fiction: Form and Genre in Susshu Foster's Atomik AztexPart 3 | Remapping the Transpacific Turn: From the Black Pacific and Oceanic Ecopoetics to



Antipodean Transnationalisms; 7 | The Manchurian Philosopher: W. E. B. Du Bois in the Eurasian Pacific; 8 | Toward an Ecopoetics of Oceania: Worlding the Asia-Pacific Region as Space-Time Ecumence; 9 | Antipodean Transnationalism: The Empire Lies Athwart; 10 | Transpacific Studies and the Cultures of US Imperialism; Part 4 | Decolonizing Knowledge Production for the Pacific Century

11 | Geopolitics of Knowing/Understanding and American Studies: A Decolonial Argument or View from the Global South12 | Industries of Memory: The Vietnam War in Art; 13 | Chinamerica: Global Affairs and Planetary Consciousness; 14 | Negociating the Technological Empire: Cosmopolitics, Colonial Modernity, and Early Chinese American Autobiographical Writing; Acknowledgements; Contributors; Index

Sommario/riassunto

The internationalization of American studies

2.

Record Nr.

UNINA9910829864203321

Titolo

Plant proteomics [[electronic resource] /] / edited by Christine Finnie

Pubbl/distr/stampa

Oxford, UK ; ; Ames, Iowa, : Blackwell Pub., 2006

ISBN

1-280-74882-6

9786610748822

0-470-76427-9

0-470-98887-8

1-4051-7307-6

Descrizione fisica

1 online resource (276 p.)

Collana

Annual plant reviews ; ; v. 28

Classificazione

42.42

Altri autori (Persone)

FinnieChristine

Disciplina

572.62

572/.62

580.5

Soggetti

Plant proteins

Plant proteomics

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Plant Proteomics; Contents; Preface; Contributors; 1 Plant proteomics:



challenges and resources; 1.1 Introduction; 1.2 Challenges; 1.2.1 Sample extraction; 1.2.1.1 Two-dimensional gel electrophoresis; 1.2.1.2 Direct MS analysis of samples; 1.2.2 Sample preparation and arraying; 1.2.2.1 Two-dimensional gel electrophoresis; 1.2.2.2 One-dimensional gel electrophoresis; 1.2.2.3 Blue-native gel electrophoresis; 1.2.2.4 Direct analysis of samples by MS; 1.2.3 Mass spectrometry (MALDI and ESI); 1.2.3.1 MALDI; 1.2.3.2 ESI; 1.2.4 Analysis depth; 1.2.5 Data analysis; 1.2.5.1 Peptide mass fingerprints

1.2.5.2 Peptide fragmentation data (MS/MS)1.2.5.3 Analysis options; 1.2.6 Quantitation; 1.2.6.1 Gel stains; 1.2.6.2 Chemical labelling of sample; 1.2.7 Modifications; 1.2.8 Data; 1.3 Resources; 1.3.1 Proteomic databases; 1.3.2 Online proteomic tools and resources; 1.4 Future; 2 Proteomic analysis of post-translational modifications by mass spectrometry; 2.1 Summary; 2.2 Introduction; 2.3 Considerations for the experimental design of PTM analysis by proteomics; 2.4 Analysis of PTMs by proteomic approaches; 2.4.1 Phosphorylation; 2.4.2 Protein glycosylation; 2.4.3 GPI-AP; 2.4.4 Farnesylation

2.4.5 N-terminally modified proteins2.5 Conclusions and perspectives; 3 Strategies for the investigation of protein-protein interactions in plants; 3.1 Summary; 3.2 Introduction; 3.3 Biochemical procedures to characterize protein-protein interactions; 3.3.1 Chromatographic purifications; 3.3.2 Sucrose gradient ultrafiltration; 3.3.3 Native gel electrophoresis; 3.3.4 Immunoprecipitations; 3.4 Genetic procedures to characterize protein-protein interactions; 3.4.1 Yeast two-hybrid system; 3.4.2 Yeast three-hybrid system; 3.4.3 Yeast one-hybrid system

3.4.4 Limitations of yeast two-hybrid systems3.4.5 Split-ubiquitin system; 3.4.6 Bimolecular fluorescence complementation (BiFC); 3.4.7 Förster resonance energy transfer (FRET); 3.4.8 Tagging technologies for the purification of protein complexes; 3.5 Cytological procedures to characterize protein-protein interactions; 3.6 Outlook; 4 Proteomics of disulphide and cysteine oxidoreduction; 4.1 Introduction; 4.2 Control of cellular redox status; 4.2.1 Sequence and structural features of proteins catalysing cysteine redox modifications; 4.2.2 Catalytic mechanisms of Trxs and Grxs

4.3 Proteomics techniques for analysis of cysteine modifications4.3.1 Reagents for cysteine labelling; 4.3.2 Disulphide mapping; 4.3.3 S-glutathionylation; 4.3.4 Cysteine SOH, SO2H and SO3H; 4.3.5 Trxs and disulphide reduction; 4.3.6 S-nitrosylation; 4.4 Conclusions and perspectives; 5 Structural proteomics; 5.1 Introduction; 5.2 Project data handling: Sesame; 5.3 ORF cloning; 5.4 E. coli cell-based protein production pipeline; 5.4.1 Large-scale protein production and labeling; 5.4.2 Protein purification; 5.5 Wheat germ cell-free protein production

5.6 Mass spectrometry of purified proteins for quality assurance and analysis

Sommario/riassunto

The proteome comprises all protein species resulting from gene expression in a cell, organelle, tissue or organism. By definition, proteomics aims to identify and characterise the expression pattern, cellular location, activity, regulation, post-translational modifications, molecular interactions, three dimensional structures and functions of each protein in a biological system.In plant science, the number of proteome studies is rapidly expanding after the completion of the Arabidopsis thaliana genome sequence, and proteome analyses of other important or emerging model systems and crop