The horizontal effect revolution and the question of sovereignity / / Johan van der Walt

• Record Nr.: UNINA9910459717303321
• Autore: Van der Walt Johan Willem Gous
• Pubbl/distr/stampa: Berlin : , : De Gruyter, , [2014]; ©2014
• ISBN: 3-11-039170-8; 3-11-024803-4
• Descrizione fisica: 1 online resource (450 p.)
• Disciplina: 341.26
• Soggetti: Civil rights - Europe; International and municipal law - Europe; Judicial review - Europe; Law - Europe - American influences; Electronic books.
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and indexes.
• Nota di contenuto: Front matter -- Preface -- Contents -- Acknowledgements and Disclaimers -- Introduction -- Part One: Horizontal Effect -- Chapter One: Erased Baselines and Inversed Coordinates: 19th Century Backgrounds of the Horizontality Question -- Chapter Two: Twelve Pivotal Cases -- Chapter Three: State Action -- Chapter Four: Drittwirkung -- Part Two: Sovereignty -- Chapter Five: Uninterrupted Sovereignty -- Chapter Six: Différantial Sovereignty -- Chapter Seven: Sovereignty and the Dual Destiny of Lüth in Europe -- Chapter Eight: Liberal Democratic Constitutional Review -- Bibliography -- Abbreviations -- Index of Persons -- Subject Index
• Sommario/riassunto: That the recent turn in European Constitutional Review has effectively brought about a revolution in European law has been observed before. At issue are two major developments in European judicial review. On the one hand, the European Court of Human Rights has been collapsing traditional boundaries between constitutional law and private law with a series of decisions that effectively recognized the "horizontal" effect of Convention rights in the private sphere. On the other hand, the ; European Court of Justice has also given horizontal effect to fundamental liberties embodied in the Treaty on the Function of the European Union in a number of recent cases in a way that puts "established" boundaries between Member State and Union competences in question. This book takes issue with these developments by bringing to the fore a key issue that the horizontality effect debate has hitherto largely overlooked, namely, the question of sovereignty. It shows with detailed references to especially the American debate on state action and the German debate on Drittwirkung that horizontal effect cannot be understood consistently without coming to grips with the conceptions of state sovereignty that inform different approaches to horizontal effect.

Day-fatality: or, Some observation of days lucky and unlucky [[electronic resource] ] : concluding with some remarques upon the fourteenth of October, the auspicious birth-day of His Royal Highness, James Duke of York

• Record Nr.: UNISA996397382603316
• Autore: Gibbon John <1629-1718.>
• Pubbl/distr/stampa: [London, : s.n., 1679]
• Descrizione fisica: 8 p
• Soggetti: Chronology, Historical
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Caption title.; Imprint suggested by Wing.; Signed: J. Gibbon.; Reproduction of original in the British Library.
• Sommario/riassunto: eebo-0062

Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel

• Record Nr.: UNINA9910143129503321
• Pubbl/distr/stampa: Weinheim, : Wiley-VCH, 2009
• ISBN: 1-282-29205-6; 9786612292057; 3-527-62668-9; 3-527-62669-7
• Descrizione fisica: 1 online resource (193 p.)
• Altri autori (Persone): KosticTanja; ButayePatrick; SchrenzelJacques
• Disciplina: 579.165
• Soggetti: Pathogenic microorganisms - Detection; DNA microarrays
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating; 1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 ; Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays; 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection; 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene; 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms; 5.1 Introduction
• Sommario/riassunto: Written by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents.