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Record Nr. |
UNINA9910457771203321 |
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Autore |
Carson Sue, Dr. |
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Titolo |
Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
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Pubbl/distr/stampa |
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Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
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ISBN |
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1-283-31097-X |
9786613310972 |
0-12-385545-4 |
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Edizione |
[3rd ed.] |
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Descrizione fisica |
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1 online resource (xxvi, 200 pages) : color illustrations |
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Altri autori (Persone) |
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MillerHeather Bennett |
WitherowD. Scott (Dennis Scott) |
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Disciplina |
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Soggetti |
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Molecular biology |
Molecular biology - Technique |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Includes index. |
Previous edition: 2006. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab |
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Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
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Sommario/riassunto |
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, |
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2. |
Record Nr. |
UNINA9910465777503321 |
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Autore |
Ribbens Benjamin J. <1983-> |
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Titolo |
Levitical sacrifice and heavenly cult in Hebrews / / Benjamin J. Ribbens |
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Pubbl/distr/stampa |
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Berlin, [Germany] ; ; Boston, [Massachusetts] : , : De Gruyter, , 2016 |
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©2016 |
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ISBN |
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3-11-047589-8 |
3-11-047713-0 |
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Descrizione fisica |
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1 online resource (316 p.) |
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Collana |
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Beihefte zur Zeitschrift für die neutestamentliche Wissenschaft, , 0171-6441 ; ; Volume 222 |
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Disciplina |
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Soggetti |
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Sacrifice - Bible teaching |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and indexes. |
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Nota di contenuto |
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Frontmatter -- Acknowledgements -- Contents -- List of Abbreviations -- 1. The Efficacy of Levitical Sacrifice Compared to Christ’s Sacrifice in |
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Hebrews -- 2. Sacrifice Theology in Second Temple Judaism -- 3. Heavenly Cult in Second Temple Judaism -- 4. Heavenly Tabernacle and Cult in Hebrews -- 5. Old Covenant Sacrifices -- 6. New Covenant Sacrifice -- 7. Conclusion -- Appendix: Perfection in Second Temple Literature and the New Testament -- Bibliography -- Index of References -- Index of Authors -- Index of Subjects |
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Sommario/riassunto |
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This monograph examines Hebrews’ understanding of the relationship between old covenant sacrifices and Christ’s new covenant sacrifice, especially as it relates to the question of efficacy. Most scholars think the author of Hebrews strips the levitical sacrifices of most, if not all, efficacy, but this work affirms a more positive depiction of the levitical sacrifices. A mystical apocalyptic tradition stands behind Hebrews’ description of the heavenly cult , which establishes the framework for relating the levitical sacrifice to Christ’s sacrifice. The earthly, levitical cult was efficacious when it corresponded to or synchronized with the heavenly sacrifice of Christ. Still, the author of Hebrews develops the notion of the heavenly cult in unique ways, as Christ’s sacrifice both validates the earthly practice but also, due to his new covenant theology, calls for its end. Ribbens’ bold proposal joins a growing number of scholars that place Hebrews in the mystical apocalyptic tradition, highlights positive statements in Hebrews related to the efficacy of levitical sacrifices that are often overlooked, and relies on the heavenly cult to reconcile the positive and negative descriptions of the levitical cult. |
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