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Record Nr. |
UNINA9910455468303321 |
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Titolo |
Subcellular fractionation [[electronic resource] ] : a practical approach / / edited by J.M. Graham and D. Rickwood |
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Pubbl/distr/stampa |
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Oxford ; ; New York, : IRL Press at Oxford University Press, c1997 |
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ISBN |
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1-283-66474-7 |
0-19-159161-0 |
0-585-48392-2 |
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Descrizione fisica |
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1 online resource (360 p.) |
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Collana |
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The practical approach series |
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Altri autori (Persone) |
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GrahamJ. M <1943-> (John M.) |
RickwoodD (David) |
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Disciplina |
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Soggetti |
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Subcellular fractionation |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Cover; Contents; List of contributors; Abbreviations; 1. Homogenization of tissues and cells; 1. Introduction; 2. Aims of the homogenization procedure; 3. Influence of sample type; 4. Homogenization media; 5. Methods of homogenization; Type 1 homogenizers; Type 2 homogenizers; 6. Homogenization of tissues and cells; Mammalian liver; Brain; Muscle; Mammalian tissue culture cells; Plant organelles; Yeast; Other fungi and algae; Trypanosomes; Bacteria; References; 2. Isolation of subcellular fractions; 1. Introduction; 2. Composition of a tissue homogenate; 3. Properties of cell organelles |
Factors affecting organelle density and size4. Centrifugal methods for the separation of organelles; Separation by size; Separation by density; Density perturbation; 5. Non-centrifugal procedures; Immunoisolation; Separation by electrophoresis; 6. Identification of separated material; Marker enzymes; Introduced markers for endocytic and exocytic pathways; Characteristic non-enzymatic proteins; 7. Assessment of the purity of fractions; Purity and purification; Problems from cell heterogeneity within tissues; Problems arising from organelle fragmentation |
Missorting in the exocytic and endocytic pathways8. Fractionation |
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problems; No separation; Aggregation following resuspension of a fraction; Poor recovery of markers; Damage to cell structures; 9. A systematic approach to cell fractionation; Preliminary studies; Determination of the properties of components of the homogenate; Method development; Simplification of the separation; References; 3. Isolation and characterization of nuclei and nuclear subfractions; 1. Introduction; 2. Methods of preparing purified nuclei; Types of cells and tissue samples; Homogenization media |
Homogenization methodsCentrifugation conditions; Assays of nuclear purity; 3. Methods for purifying metaphase chromosomes; 4. Isolation of nuclear subfractions; Preparation of nucleoli; Preparation of nuclear membranes; Isolation of nuclear matrix; Preparation of nucleoids; 5. Isolation of nucleoprotein complexes; Isolation of polynucleosomes of chromatin; Ribonucleoproteins; 6. Isolation of nuclear macromolecules; Isolation of nuclear proteins; Isolation of nuclear RNA; Isolation of DNA; 7. Functional assays of nuclei; Analysis of DNA-binding proteins; Transcription assays; References |
4. Subcellular fractionation of mitochondria1. Introduction; 2. Purification of mitochondria from various eukaryotic sources; Introduction; Protocols for purification of mitochondria from several eukaryotic sources; Further purification of mitochondrial fractions; 3. Determination of mitochondrial purity; Introduction; Use of the oxygen electrode to determine mitochondrial integrity; Determination of the integrity of the mitochondrial outer membrane; Glucose hexokinase trap method for estimation of P:O ratios; 4. Subfractionation of mitochondria |
Preparation of submitochondrial particles by sonication |
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Sommario/riassunto |
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Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles. |
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2. |
Record Nr. |
UNINA9910782816603321 |
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Titolo |
Confronting crime : crime control policy under New Labour / / edited by Michael Tonry |
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Pubbl/distr/stampa |
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Oxon [England] : , : Routledge, , 2011 |
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ISBN |
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1-134-02837-7 |
1-315-06558-4 |
1-134-02830-X |
1-281-33156-2 |
9786611331566 |
1-84392-432-3 |
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Descrizione fisica |
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1 online resource (264 p.) |
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Collana |
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Cambridge Criminal Justice Series |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Criminal justice, Administration of - Great Britain |
Crime prevention - Great Britain |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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First published by Willan Pub., 2003. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Cover; Title; Copyright; Contents; Notes on contributors; Preface; 1 Evidence, elections and ideology in the making of criminal justice policy; 2 Drug-dependent offenders and Justice for All; 3 Unprincipled sentencing? The policy approach to dangerous sex offenders; 4 Nuisance offenders: scoping the public policy problems; 5 Procedural and evidential protections in the English courts; 6 Sentencing guidelines; 7 Sentence management: a new role for the judiciary?; 8 Is sentencing in England and Wales institutionally racist?; 9 Custody plus, custody minus; 10 Reducing the prison population |
11 'Justice for All': A summary of Cambridge conference discussionsIndex |
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Sommario/riassunto |
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From Labour's promise to be 'tough on crime, tough on the causes of crime' through to the White Paper and new criminal justice legislation, controlling crime and reforming the criminal justice system has been one of the government's key priorities.This book provides a detailed review of the thinking behind these new plans and legislation, looking at policies and proposals in the field of punishment, particularly those |
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embodied in the Halliday Review of the Sentencing Framework (2001), the government White Paper Justice for All (2002), and the 2002 Criminal Justice Bill. The contribu |
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