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1. |
Record Nr. |
UNINA9910145261503321 |
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Autore |
Albani Jihad René <1956-> |
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Titolo |
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
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Pubbl/distr/stampa |
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Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
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ISBN |
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1-281-31900-7 |
9786611319007 |
0-470-69205-7 |
0-470-69133-6 |
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Descrizione fisica |
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1 online resource (270 p.) |
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Disciplina |
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Soggetti |
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Fluorescence spectroscopy |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer |
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions |
3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption |
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spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube |
4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References |
5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye |
6.1 Definition of pK |
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Sommario/riassunto |
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Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence |
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2. |
Record Nr. |
UNINA9910407716603321 |
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Autore |
Pollitt Michael G. |
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Titolo |
Reforming the Chinese Electricity Supply Sector : Lessons from Global Experience / / by Michael G. Pollitt |
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Pubbl/distr/stampa |
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Cham : , : Springer International Publishing : , : Imprint : Palgrave Macmillan, , 2020 |
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ISBN |
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Edizione |
[1st ed. 2020.] |
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Descrizione fisica |
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1 online resource (xxvi, 260 pages) : illustrations |
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Disciplina |
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Soggetti |
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Business |
Asia |
Energy policy |
Power resources |
International economic integration |
Globalization |
Asian Business |
Energy Policy, Economics and Management |
Natural Resource and Energy Economics |
Emerging Markets and Globalization |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di contenuto |
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1. An Introduction to the Chinese Power System and its Reform -- 2. Lessons for China from International Experience of Power Sector Reform -- 3. Power Market Reform in China - Lessaon from Guangdong -- 4. How Industrial electricity prices are determined in a reformed power market: lessons from Great Britain for China -- 5. Prospects for Reform of China's electric power sector. |
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Sommario/riassunto |
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The Chinese electricity sector is the largest in the world, covering well over 20% of the world's electricity supply. While many other countries liberalized their electricity systems in the 1990s, thereby creating competitive wholesale and retail electricity markets, China's move |
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towards liberalization has advanced at a slower pace - until now. Following the China State Council's publication of the No. 9 document on 'Deepening Reform of the Power Sector', this book reflects on the ambitious new round of reforms aimed at introducing competitive wholesale electricity markets and incentive regulation for its power grids. Written in collaboration with Hao Chen, Lewis Dale and Chung-Han Yang, this book provides lessons for China's reforms from international experience, combining a detailed review of reforms from around the world with specific application to China and focuses on how the industrial price of electricity is determined in a liberalized power system. |
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