Computational and statistical methods for protein quantification by mass spectrometry / / Ingvar Eidhammer ... [et al.]

• Record Nr.: UNINA9911020318803321
• Autore: Eidhammer Ingvar
• Pubbl/distr/stampa: Chichester, West Sussex, U.K., : John Wiley & Sons Inc., 2013
• ISBN: 9781118494042; 1118494040; 9781299188266; 1299188265; 9781118493786; 1118493788; 9781118493779; 111849377X
• Descrizione fisica: 1 online resource (356 p.)
• Disciplina: 572/.636
• Soggetti: Proteomics - Statistical methods; Mass spectrometry - Data processing
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Computational and Statistical Methods for Protein Quantification by Mass Spectrometry; Contents; Preface; Terminology; Acknowledgements; 1 Introduction; 1.1 The composition of an organism; 1.1.1 A simple model of an organism; 1.1.2 Composition of cells; 1.2 Homeostasis, physiology, and pathology; 1.3 Protein synthesis; 1.4 Site, sample, state, and environment; 1.5 Abundance and expression - protein and proteome profiles; 1.5.1 The protein dynamic range; 1.6 The importance of exact specification of sites and states; 1.6.1 Biological features; 1.6.2 Physiological and pathological features; 1.6.3 Input features1.6.4 External features; 1.6.5 Activity features; 1.6.6 The cell cycle; 1.7 Relative and absolute quantification; 1.7.1 Relative quantification; 1.7.2 Absolute quantification; 1.8 In vivo and in vitro experiments; 1.9 Goals for quantitative protein experiments; 1.10 Exercises; 2 Correlations of mRNA and protein abundances; 2.1 Investigating the correlation; 2.2 Codon bias; 2.3 Main results from ; experiments; 2.4 The ideal case for mRNA-protein comparison; 2.5 Exploring correlation across genes; 2.6 Exploring correlation within one gene; 2.7 Correlation across subsets; 2.8 Comparing mRNA and protein abundances across genes from two situations2.9 Exercises; 2.10 Bibliographic notes; 3 Protein level quantification; 3.1 Two-dimensional gels; 3.1.1 Comparing results from different experiments - DIGE; 3.2 Protein arrays; 3.2.1 Forward arrays; 3.2.2 Reverse arrays; 3.2.3 Detection of binding molecules; 3.2.4 Analysis of protein array readouts; 3.3 Western blotting; 3.4 ELISA - Enzyme-Linked Immunosorbent Assay; 3.5 Bibliographic notes; 4 Mass spectrometry and protein identification; 4.1 Mass spectrometry; 4.1.1 Peptide mass fingerprinting (PMF); 4.1.2 MS/MS - tandem MS4.1.3 Mass spectrometers; 4.2 Isotope composition of peptides; 4.2.1 Predicting the isotope intensity distribution; 4.2.2 Estimating the charge; 4.2.3 Revealing isotope patterns; 4.3 Presenting the intensities - the spectra; 4.4 Peak intensity calculation; 4.5 Peptide identification by MS/MS spectra; 4.5.1 Spectral comparison; 4.5.2 Sequential comparison; 4.5.3 Scoring; 4.5.4 Statistical significance; 4.6 The protein inference problem; 4.6.1 Determining maximal explanatory sets; 4.6.2 Determining minimal explanatory sets; 4.7 False discovery rate for the identifications; 4.7.1 Constructing the decoy database4.7.2 Separate or composite search; 4.8 Exercises; 4.9 Bibliographic notes; 5 Protein quantification by mass spectrometry; 5.1 Situations, protein, and peptide variants; 5.1.1 Situation; 5.1.2 Protein variants - peptide variants; 5.2 Replicates; 5.3 Run - experiment - project; 5.3.1 LC-MS/MS run; 5.3.2 Quantification run; 5.3.3 Quantification experiment; 5.3.4 Quantification project; 5.3.5 Planning quantification experiments; 5.4 Comparing quantification approaches/methods; 5.4.1 Accuracy; 5.4.2 Precision; 5.4.3 Repeatability and reproducibility; 5.4.4 Dynamic range and linear dynamic range
• Sommario/riassunto: The definitive introduction to data analysis in quantitative proteomics This book provides all the necessary knowledge about mass spectrometry based proteomics methods and computational and statistical approaches to pursue the planning, design and analysis of quantitative proteomics experiments. The author's carefully constructed approach allows readers to easily make the transition into the field of quantitative proteomics. Through detailed descriptions of wet-lab methods, computational approaches and statistical tools, this book covers the full scope of a quantitative experim

Handbook of Argumentation Theory / / by Frans H. van Eemeren, Bart Garssen, Bart Verheij, Erik C. W. Krabbe, A. Francisca Snoeck Henkemans, Jean H. M. Wagemans

• Record Nr.: UNINA9910390860503321
• Autore: Eemeren F. H. van (Frans Hendrik), <1946->
• Pubbl/distr/stampa: Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2020
• ISBN: 94-007-6883-4
• Descrizione fisica: 1 online resource (600 p.)
• Disciplina: 100
• Soggetti: Philosophy; Linguistics; Social sciences; Law; Logic; Philosophy, general; Linguistics, general; Social Sciences, general; Law, general
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Nota di contenuto: Preface.. -1. Introduction. -- 2. Classical Backgrounds. -- 3. Modern Backgrounds. -- 4. The Toulmin Model. -- 5. The New Rhetoric. -- 6. Formal Approaches. -- 7. Informal Logic. -- 8. Communication and Rhetoric in the United States. -- 9. Linguistic Approaches. -- 10. Pragma-Dialectics. -- 11. Other Angles and Approaches. -- 12. Argumentation in Practice. -- References. -- Author Index. -- Subject Index .
• Sommario/riassunto: The Handbook Argumentation Theory provides an up to date survey of the various theoretical contributions to the development of argumentation theory for all scholars interested in argumentation, informal logic and rhetoric. It describes the historical roots of modern argumentation theory that are still an important theoretical background to contemporary approaches. Because of the complexity, diversity and ; rate of developments in argumentation theory, there is a real need for an overview of the state of the art, the main approaches that can be distinguished and the distinctive features of these approaches. The Handbook covers classical and modern backgrounds to the study of argumentation, the New Rhetoric developed by Perelman and Olbrechts-Tyteca, the Toulmin model, formal approaches, informal logic, communication and rhetoric, pragmatic approaches, linguistic approaches and pragma-dialectics. The Handbook is co-authored by Frans H. van Eemeren, Bart Garssen, Erik C.W. Krabbe, A. Francisca Snoeck Henkemans, Bart Verheij and Jean Wagemans, who are a coherent and prominent writing team whose expertise covers the whole field. The authors are assisted by an international Editorial Board consisting of outstanding argumentation scholars whose fields of interest are represented in the volume. .

The Sol to Gel Transition / / by Plinio Innocenzi

• Record Nr.: UNINA9910254047103321
• Autore: Innocenzi Plinio
• Pubbl/distr/stampa: Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016
• ISBN: 3-319-39718-4
• Edizione: [1st ed. 2016.]
• Descrizione fisica: 1 online resource (X, 76 p. 56 illus., 35 illus. in color.)
• Collana: SpringerBriefs in Materials, , 2192-1105
• Disciplina: 541.345
• Soggetti: Ceramic materials; Physical chemistry; Soft condensed matter; Microtechnology; Microelectromechanical systems; Inorganic chemistry; Optical materials; Ceramics; Physical Chemistry; Soft and Granular Matter; Microsystems and MEMS; Inorganic Chemistry; Optical Materials
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Nota di bibliografia: Includes bibliographical references at the end of each chapters and index.
• Nota di contenuto: A Sol and a Gel, What They Are? -- From the Precursor to a Sol -- Sol-to-Gel Transition: The Models -- From Silicate Oligomers to Gelation -- Measuring the Sol to Gel Transition -- Probing the Sol-to-Gel Transition in the GeL Structure -- Conclusion.
• Sommario/riassunto: This book provides an in-depth introduction to the sol to gel transition in inorganic and hybrid organic-inorganic systems, one of the most important chemical-physical transitions and the basis of the sol-gel process. Familiarity with the fundamental chemistry and physics of this transition is essential for students in chemistry and materials science through academic and industry researchers working on sol-gel-related applications. The book features a didactic approach, using simple and clear language to explain the sol to gel transition and the accompanying processes. The text is also suitable for use in short courses and workshops for graduate students as well as professionals.