1.

Record Nr.

UNINA9910300541203321

Titolo

Cellular Imaging : Electron Tomography and Related Techniques / / edited by Eric Hanssen

Pubbl/distr/stampa

Cham : , : Springer International Publishing : , : Imprint : Springer, , 2018

ISBN

3-319-68997-5

Edizione

[1st ed. 2018.]

Descrizione fisica

1 online resource (XIX, 323 p. 84 illus., 59 illus. in color.)

Collana

Biological and Medical Physics, Biomedical Engineering, , 1618-7210

Disciplina

616.07572

Soggetti

Biophysics

Biological physics

Spectroscopy

Microscopy

Biomedical engineering

Biological and Medical Physics, Biophysics

Spectroscopy and Microscopy

Biomedical Engineering and Bioengineering

Spectroscopy/Spectrometry

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Nota di bibliografia

Includes bibliographical references at the end of each chapters and index.

Nota di contenuto

TEM Tomography -- STEM Tomography -- CryoTomo -- Large Volume Electron Tomography -- Block Face Tomography -- Array Tomography -- Image Alignment -- Reconstruction Algorithms -- Subtomogram Averaging -- Resolution -- Signal Optimisation -- Segmentation -- Placing Macromolecular Complexes in Context.

Sommario/riassunto

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging).  The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like



scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation.