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1. |
Record Nr. |
UNINA990004315240403321 |
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Autore |
Wisskirchen, Rotruat <1936-> |
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Titolo |
Das Mosaikprogramm von S. Prassede in Rom : Ikonographie und Ikonologie / von Rotraut Wisskirken |
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Pubbl/distr/stampa |
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Munster : Aschendorff, 1990 |
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ISBN |
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3-402-08531-3 |
3-402-08530-5 |
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Descrizione fisica |
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156 p.; 8, 28 , 1 c. di tav. + ill. ; 28 cm |
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Collana |
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Jahrbuch für Antike und Christentum. Ergänzungsband ; 17 |
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Disciplina |
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Locazione |
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Collocazione |
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738.5 WIS 1 |
738.5 WIS 1 BIS |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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2. |
Record Nr. |
UNINA9910561300603321 |
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Autore |
Mandal Santi M. |
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Titolo |
Automation and basic techniques in medical microbiology / / Santi M. Mandal and Debarati Paul |
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Pubbl/distr/stampa |
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Gateway East, Singapore : , : Springer, , [2022] |
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©2022 |
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ISBN |
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Descrizione fisica |
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1 online resource (213 pages) |
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Disciplina |
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Soggetti |
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Medical microbiology |
Microbiology - Technique |
Microbiologia mèdica |
Metodologia de la ciència |
Llibres electrònics |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Intro -- Preface -- Acknowledgements -- Contents -- About the Authors -- 1: Good Laboratory Practices -- 1.1 Introduction -- 1.2 Basic Record and Lab Note Book -- 1.3 Laboratory Safety Equipment -- 1.4 Biosafety Levels and Practices -- 2: Automation in Medical Microbiology -- 2.1 Introduction -- 2.2 Applications of Automation -- 2.3 Advantages and Disadvantages -- 2.3.1 Advantages of Using Auto-analysers -- 2.3.2 Disadvantages of Automation -- 2.4 Types of Auto-analysers -- 2.5 History of Auto-analysers -- 2.6 Laboratory Automation and Total Laboratory Automation -- 2.7 Types and Applications of Auto-analysers in Microbiology -- 2.7.1 Microbiological Specimen Processor -- 2.7.2 Routine Biochemistry Analysers -- 2.7.3 Immunology-Based Analysers -- 2.7.4 Haematology Analysers -- 2.7.5 Cell Counter -- 2.7.6 Coagulometer(s) -- 2.7.7 Additional Instrument for Haematology-Based Methods -- 2.7.8 Other Miscellaneous Analysers -- References -- 3: Manual and Automated Characterization of Multi-antibiotic-Resistant (MAR) Bacteria -- 3.1 Introduction -- 3.2 Types of Antibiotic Sensitivity Tests -- 3.2.1 Kirby-Bauer Disc Diffusion Method -- 3.2.2 The Minimum Inhibitory Concentration (MIC) Method |
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-- 3.2.3 RAPD PCR Analysis -- 3.2.4 Multiplex PCR -- 3.2.5 Padlock PCR and Microarray Analysis -- 3.2.6 Real-Time PCR for Quantitative Data -- References -- 4: Rapid Microbial Genome Sequencing Techniques and Applications -- 4.1 Introduction -- 4.2 WGS Techniques -- 4.3 Data Analysis -- Protocol for WGS (adapted from Gautam et al. 2019) -- 4.4 Applications -- 4.5 Challenges -- References -- 5: Spectroscopy: Principle, Types and Microbiological Applications -- 5.1 Introduction -- 5.2 General Types of Spectra -- 5.2.1 Continuous Spectra -- 5.2.2 Discrete Spectra -- 5.2.2.1 Emission Line Spectra -- 5.2.2.2 Absorption Line Spectra -- 5.3 Principle of Spectroscopy. |
5.4 Optical Instruments in Spectroscopy -- 5.5 Is Spectroscopy Different from Spectrometry? -- 5.6 Uses of Spectroscopy -- 5.7 Types of Spectroscopy -- 5.7.1 Ultraviolet and Visible Spectroscopy -- 5.7.1.1 Background -- 5.7.1.2 Principle -- 5.7.1.3 Applications of UV-Vis Spectroscopy -- 5.7.1.3.1 Spectroscopy in Environmental Analysis -- 5.7.1.3.2 UV-Vis Spectroscopy for Water Analysis and Environmental Applications -- 5.7.1.3.3 Spectrophotometric Analysis of Bacterial Water Contaminants -- 5.7.1.3.4 Spectrophotometers for Chlorine and Flouride Quantification -- 5.7.1.3.5 UV-Vis Spectroscopy for Geological Studies Linked to Water Contamination -- 5.7.1.3.6 Other Applications -- 5.7.2 Infrared Spectroscopy -- 5.7.2.1 Introduction -- 5.7.2.1.1 Molecular Vibrations and Vibrational Frequency -- 5.7.2.1.1.1 Vibration of Diatomic Molecules -- 5.7.2.1.1.2 Vibrational Transitions -- 5.7.2.1.1.3 Types of Vibrations (Sharma 2007) -- 5.7.2.2 Instrumentation -- 5.7.2.2.1 Source -- 5.7.2.2.2 Sample Types and Preparation -- 5.7.2.2.3 Various Types of Detectors Used -- 5.7.2.3 FTIR (Fourier Transform IR Spectrometers) -- 5.7.2.4 Advantages of FTIR -- 5.7.2.5 Applications of IR Spectroscopy -- 5.7.3 Mass Spectrometry -- 5.7.3.1 The Mass Spectrometer -- 5.7.3.2 The Nature of Mass Spectra -- 5.7.3.3 The Working Principle of a Mass Spectrometer -- 5.7.3.4 Applications of Mass Spectrometry -- 5.7.3.4.1 Analysis of Biomolecules -- 5.7.3.4.2 Analysis of Glycans -- 5.7.3.4.3 Analysis of Lipids -- 5.7.3.4.4 Analysis of Proteins and Peptides -- 5.7.3.4.5 Analysis of Oligonucleotides -- 5.7.4 Nuclear Magnetic Resonance (NMR) Spectroscopy -- 5.7.4.1 NMR Spectrum -- 5.7.4.2 NMR Spectrometers -- 5.7.4.3 Applications of NMR -- 5.8 Applications of Spectroscopy in Microbiology -- References -- 6: MALDI-TOF MS for Bacterial Identification -- 6.1 Introduction. |
6.2 MALDI: Sample Preparation and Analysis -- 6.2.1 Sample Preparation -- 6.2.2 Protein Digestion -- 6.2.3 MALDI/MS Analysis -- 6.3 Uses of MALDI-TOF -- 6.4 MALDI-TOF MS-Based Antimicrobial Susceptibility Testing -- 6.4.1 Detection of Antibiotic Degradation -- 6.4.2 Identification of Biomarker for Detecting Antibiotic-Resistant Strains -- 6.4.3 Phenotypic Antibiotic Resistance Analysis of Bacterial Strains -- 6.5 Advantages and Limitations -- 6.6 Challenges -- References -- 7: Enzyme-Linked Immunosorbent Assay (ELISA) -- 7.1 Introduction -- 7.2 Indirect ELISA -- 7.2.1 Steps of Indirect ELISA -- 7.3 Direct or Sandwich ELISA -- 7.3.1 Steps of Double Antibody Sandwich (DAS) ELISA -- 7.3.2 Steps of Triple Antibody Sandwich (TAS) ELISA -- 7.4 Competitive ELISA -- 7.5 Radioimmunoassay (RIA) -- 7.5.1 Steps of RIA -- 7.6 Automated ELISA -- References -- 8: Isolation of Normal Microbiota from the Human Body and Microbial Identification -- 8.1 Introduction -- 8.2 Collection of Samples from Various Parts of the Body -- 8.3 Biochemical Tests for Identification of Bacteria -- 8.3.1 Carbohydrate Fermentation -- 8.3.2 Indole Production Test -- 8.3.3 Methyl Red Test -- 8.3.4 Voges-Proskauer Test -- 8.3.5 Citrate Utilization -- 8.3.6 Urease Test -- 8.3.7 Catalase Test -- 8.3.8 |
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Coagulase Test -- 8.3.9 Lactophenol Cotton Blue -- 8.4 Rapid Multitest Systems -- 8.4.1 Automated Validation of Every Result (VITEK) System for Microbial Identification -- 8.4.2 Biolog: Phenotype Microarrays -- 8.4.3 Electromigration Techniques -- 8.4.4 MIDI Sherlock System for FAME Analysis -- 8.5 Computer-Aided Gene Analysis for Identification of Microbes -- 8.5.1 Ribosomal RNA Gene Sequencing -- 8.5.2 Phylogenetic Analysis -- 8.5.3 Generating Multiple Sequence Alignments -- 8.6 Conclusion -- References -- 9: Microarrays and Its Application in Medical Microbiology -- 9.1 Introduction. |
9.2 Basic Principle -- 9.3 Immobilization Strategies Used for Preparing Microarrays -- 9.4 Manufacture of the Different Components of Microarrays -- 9.4.1 Oligonucleotide Synthesis -- 9.5 Properties of Fluorescence and Fluorophores -- 9.6 Measuring Fluorescence -- 9.7 Labelling Samples for Analysis of Gene Expressions -- 9.8 Labelling Strategies -- 9.8.1 Labelling Bacterial Transcripts -- 9.9 Labelling Samples for Gene Expression Microarray -- 9.10 Calculating Label Density in Probe -- 9.11 Steps for Microarray Hybridization -- 9.12 Different Slide Types for Microarray -- 9.13 Comparing Automated and Manual Hybridization (Table 9.2) -- 9.14 Imaging for Microarray System -- 9.15 Optical System for Imaging in Microarray -- 9.16 Detector System, Amplifier System and Digital Resolution for Imaging in Microarray -- 9.17 Scanners and Excitation Light System for Microarray -- 9.18 Data Analysis in Microarray -- 9.19 Normalization of Data for Correcting Experimental Variation Between Slides -- 9.20 Visualizing of Data and Clustering -- 9.21 Troubleshooting During Microarray-Based Experiments -- 9.22 Applications of Microarrays -- 9.23 Limitations of Microarray Technique -- 9.24 Conclusion and Future Direction -- References -- 10: Immunotechnology -- 10.1 Introduction -- 10.1.1 Monoclonal Antibodies: Purification and Concentrate -- 10.1.1.1 Principle -- 10.1.1.2 Method -- 10.1.2 Concentrate the Purified Antibody -- 10.1.3 Analysis and Quality Assurance -- 10.1.4 Preparation of Separation Gel -- 10.1.5 Preparation of Protein Sample and Loading -- 10.1.6 Staining and Distaining of the Gel -- 10.1.7 Quality Assurance -- 10.2 Immunoelectrophoresis -- 10.2.1 Protocol -- 10.3 Western Blotting -- 10.3.1 Required Material -- 10.3.2 Protocol -- 10.3.3 Blocking of Membrane -- 10.3.4 Binding of Primary Antibody -- 10.3.5 Binding of Secondary Antibody. |
10.4 Determination of Cell Number -- 10.4.1 Required Material -- 10.4.2 Method -- 10.5 Immunofluorescence Assay -- 10.5.1 Principle -- 10.5.2 Immunofluorescence Technique -- 10.5.3 Labelling of Antibodies with Fluorochromes -- 10.5.4 Detection of Fluorochrome-Labelled Reagent -- 10.5.5 Selection of Fluorochrome -- 10.5.6 Materials -- 10.5.7 Blocking Buffer -- 10.5.8 Dilution Buffer -- 10.5.9 Fixative Solution -- 10.5.10 Immunostaining -- 10.5.11 Immunofluorescence Staining Method -- 10.5.12 Uses -- References -- 11: Advances in Microscopy -- 11.1 Introduction -- 11.2 Light Microscopy -- 11.2.1 Physical Properties of Light -- 11.2.2 Reflection -- 11.2.3 Transmission -- 11.2.4 Absorption -- 11.2.5 Refraction -- 11.2.6 Diffraction -- 11.2.7 The Human Eye -- 11.2.8 Polarization -- 11.2.9 Fluorescence -- 11.2.10 Important Concepts in Microscopy -- 11.2.11 Contrast -- 11.2.12 Magnification -- 11.2.13 Sensitivity -- 11.2.14 Simple Theory of Microscopy -- 11.2.15 Metric Units Used in Microscopy -- 11.2.16 Light Microscopes -- 11.2.16.1 The Compound Light Microscope -- 11.2.16.2 Inverted Microscope -- 11.3 Dark Field Microscope -- 11.4 Phase Contrast Microscopy -- 11.5 Differential Interference Contrast Microscopy (DIC) -- 11.6 Fluorescence Microscopy -- 11.6.1 Fluorescent Antibody Technique or Immunofluorescence -- 11.6.2 Identification of Chromosome -- |
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11.6.2.1 Fluorescence In Situ Hybridization (FISH) -- 11.7 Polarization Microscopy -- 11.8 Confocal Microscopy -- 11.9 Electron Microscopy -- 11.9.1 Introduction -- 11.9.2 Transmission Electron Microscope (TEM) -- 11.9.3 Scanning Electron Microscope -- 11.9.4 Scanning Tunneling Microscope (STM) -- 11.9.5 Atomic Force Microscope -- 11.9.6 Sample Preparation for Light Microscope -- 11.9.6.1 Wet Mount Method -- 11.9.7 Histological Techniques -- 11.9.8 Sample Preparation for Electron Microscope. |
11.9.9 Sample Preparation of TEM and SEM. |
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3. |
Record Nr. |
UNINA9910300465603321 |
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Autore |
Coughlan Michael |
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Titolo |
Beginning COBOL for Programmers / / by Michael Coughlan |
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Pubbl/distr/stampa |
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Berkeley, CA : , : Apress : , : Imprint : Apress, , 2014 |
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ISBN |
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Edizione |
[1st ed. 2014.] |
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Descrizione fisica |
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1 online resource (575 p.) |
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Collana |
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Disciplina |
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004 |
005.11 |
005.13 |
005.13/3 |
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Soggetti |
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Programming languages (Electronic computers) |
Software engineering |
Computer programming |
Programming Languages, Compilers, Interpreters |
Software Engineering/Programming and Operating Systems |
Programming Techniques |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Nota di contenuto |
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""Contents at a Glance""; ""Contents""; ""About the Author""; ""About the Technical Reviewer""; ""Acknowledgments""; ""Preface""; ""Chapter 1: Introduction to COBOL""; ""What Is COBOL?""; ""COBOL�s Target Application Domain""; ""COBOL�s Fitness for Its Application |
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Domain""; ""History of COBOL""; ""Beginnings""; ""COBOL Standards""; ""COBOL ANS 68""; ""COBOL ANS 74 (External Subprograms)""; ""COBOL ANS 85 (Structured Programming Constructs)""; ""COBOL ANS 2002 (OO Constructs)""; ""The Argument for COBOL (Why COBOL?)""; ""Dominance of COBOL in Enterprise Computing"" |
""Danger, Difficulty, and Expense of Replacing Legacy COBOL Applications""""Replacement with a COTS Package""; ""Complete Rewrite""; ""Automatic Language Conversion""; ""Wrapping the Legacy System""; ""Code Renovation""; ""Migration to Commodity Hardware and Software""; ""Shortage of COBOL Programmers: Crisis and Opportunity""; ""COBOL: The Hidden Asset""; ""Advantages of Bespoke Software""; ""Characteristics of COBOL Applications""; ""COBOL Applications Can Be Very Large""; ""COBOL Applications Are Very Long-Lived""; ""COBOL Applications Often Run in Critical Areas of Business"" |
""COBOL Applications Often Deal with Enormous Volumes of Data""""Characteristics of COBOL""; ""COBOL Is Self-Documenting""; ""COBOL Is Stable""; ""COBOL Is Simple""; ""COBOL Is Nonproprietary""; ""COBOL Is Maintainable""; ""Summary""; ""References""; ""Chapter 2: COBOL Foundation""; ""COBOL Idiosyncrasies""; ""COBOL Syntax Metalanguage""; ""Some Notes on Syntax Diagrams""; ""Example Metalanguage""; ""Divisions""; ""Sections""; ""Paragraphs""; ""Sentences""; ""Statements""; ""Structure of COBOL Programs""; ""The Four Divisions""; ""IDENTIFICATION DIVISION""; ""ENVIRONMENT DIVISION"" |
""DATA DIVISION""""File Section""; ""Working-Storage Section""; ""Data Hierarchy""; ""PROCEDURE DIVISION""; ""Shortest COBOL Program""; ""COBOL Coding Rules""; ""Name Construction""; ""Comments about Naming""; ""Comments about Program Formatting""; ""Comments about Programming Style""; ""Example Programs""; ""The COBOL Greeting Program""; ""The DoCalc Program""; ""The Condition Names Program""; ""Chapter Exercise""; ""Where to Get a COBOL Compiler""; ""Micro Focus Visual COBOL""; ""OpenCOBOL""; ""Raincode COBOL""; ""Compileonline COBOL""; ""Fujitsu NetCOBOL""; ""Summary""; ""References"" |
""Chapter 3: Data Declaration in COBOL""""Categories of Program Data""; ""COBOL Literals""; ""Alphanumeric Literals""; ""Numeric Literals""; ""Data Items (Variables)""; ""Data Type Enforcement""; ""Figurative Constants""; ""Elementary Data Items""; ""Declaring Elementary Data Items""; ""PICTURE Clause Symbols""; ""PICTURE Clause Notes""; ""Example Declarations""; ""Assignment in COBOL""; ""The MOVE Verb""; ""MOVE Syntax""; ""MOVE Rules""; ""MOVE Combinations""; ""MOVE Examples""; ""Alphanumeric MOVEs""; ""Numeric MOVEs""; ""Example Set 1""; ""Example Set 2""; ""Structured Data"" |
""Group Data Items"" |
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Sommario/riassunto |
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Beginning COBOL for Programmers is a comprehensive, sophisticated tutorial and modular skills reference on the COBOL programming language for established programmers. This book is for you if you are a developer who would like to—or must—add COBOL to your repertoire. Perhaps you recognize the opportunities presented by the current COBOL skills crisis, or are working in a mission critical enterprise which retains legacy COBOL applications. Whatever your situation, Beginning COBOL for Programmers meets your needs as an established programmer moving to COBOL. Beginning COBOL for Programmers includes comprehensive coverage of ANS 85 COBOL features and techniques, including control structures, condition names, sequential and direct access files, data redefinition, string handling, decimal arithmetic, subprograms, and the report writer. The final chapter includes a substantial introduction to object-oriented COBOL. Benefiting from over one hundred example programs, you’ll receive an |
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extensive introduction to the core and advanced features of the COBOL language and will learn to apply these through comprehensive and varied exercises. If you've inherited some legacy COBOL, you’ll be able to grasp the COBOL idioms, understand the constructs, and recognize what's happening in the code you’re working with. Today’s enterprise application developers will find that COBOL skills open new—or old—doors, and this extensive COBOL reference is the book to help you acquire and develop your COBOL skills. |
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