1.

Record Nr.

UNINA9910298658003321

Titolo

Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 1 [[electronic resource] /] / edited by GĂ©rald Thouand, Robert Marks

Pubbl/distr/stampa

Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014

ISBN

3-662-43385-0

Edizione

[1st ed. 2014.]

Descrizione fisica

1 online resource (V, 206 p. 55 illus., 24 illus. in color.)

Collana

Advances in Biochemical Engineering/Biotechnology, , 0724-6145 ; ; 144

Disciplina

572.4358

Soggetti

Microbiology

Applied Microbiology

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Bibliographic Level Mode of Issuance: Monograph

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Intro -- Contents -- Part IFundamentals of Bioluminescence -- 1 Eco-Evo Bioluminescence on Land and in the Sea -- Abstract -- 1...Introduction -- 2...Function of Bioluminescence -- 2.1 Self-defense -- 2.2 Light Organ Lure -- 2.3 Intraspecific Communication -- 2.4 Camouflage -- 2.5 Searchlight -- 2.6 Functionless Bioluminescence -- 3...Terrestrial Taxa -- 3.1 On Land -- 3.2 In the Air -- 3.3 Underground: Why in Soil? -- 3.4 Fresh Water: Why so Rare? -- 4...Marine Taxa -- 4.1 Intertidal Zone -- 4.2 Coastal Water -- 4.2.1 Cypridinid Luciferin -- 4.3 Midwater: Why so Species-Rich? -- 4.3.1 Symbiotic Luminescence -- 4.3.2 Coelenterazine -- 4.4 Deep-Sea Benthos -- 5...Conclusion -- 6...Perspectives -- Acknowledgments -- References -- 2 Biochemistry and Genetics of Bacterial Bioluminescence -- Abstract -- 1...Introduction -- 2...Species of Luminous Bacteria -- 3...Biochemistry of Bacterial Luminescence -- 4...Bacterial lux Genes -- 5...Regulation of lux Operon Expression -- 6...Origin and Function of Luminescence in Bacteria -- 7...Outlook -- References -- Part IIApplications of Bioluminescence inEnvironment and Security -- 3 Application of Enzyme Bioluminescence in Ecology -- Abstract -- 1...Introduction -- 2...Principles of Bioluminescent Enzymatic Bioassays -- 2.1 The Bioassays Based on the Bacterial Coupled Enzyme System:



NADH:FMN-Oxidoreductase-Luciferase -- 2.2 Enzymatic Reagents for Bioluminescent Analysis -- 2.2.1 Ways to Obtain Stable Enzyme Preparations -- 2.2.2 Design of the Reagent Enzymolum and the Procedure of Toxicological Bioassay with Increased Sensitivity to Pollutants -- Varying the Composition of the Immobilized Reagent -- Varying the Order of Components Added to the Reaction Mixture -- Incubating Enzymes in the Test Solutions -- Comparing Sensitivity of Soluble and Multicomponent Immobilized Coupled Enzyme System NADH:FMN-Oxidoreductase-Luciferase.

2.3 Biotesting Natural and Waste Waters Using the Soluble and Immobilized Coupled Enzyme System NADH: FMN-Oxidoreductase-Luciferase -- 2.4 The Reagent ''Enzymolum'' in Toxicological Bioassay of Air -- 2.5 Signal System of Enzymatic Assays: Methodological Aspects -- 2.6 How to Design New Bioluminescent Enzymatic Bioassays -- 2.7 Signal System of Enzymatic Methods for Toxicological Bioassay of Natural and Laboratory Aquatic Ecosystems -- 2.7.1 Dynamics of the Pond Ecosystem and the Microecosystem Components and the Bioassay Data -- 2.7.2 Bioluminescent Control for Blooming Water -- 2.8 Signal System of Enzymatic Assays for Toxicological Bioassay of Pesticides -- 3...Summary -- Acknowledgments -- References -- 4 Detection of Organic Compounds with Whole-Cell Bioluminescent Bioassays -- Abstract -- 1...Introduction -- 2...Detection of Organic Compounds Using Bacterial Bioluminescent Assays -- 2.1 Benzene, Toluene, Ethylbenzene, and Xylene -- 2.2 Polycyclic Aromatic Hydrocarbons -- 2.3 Alkane Aliphatic Hydrocarbons -- 2.4 Chlorinated Aliphatic Hydrocarbons -- 2.5 Biphenyl and Polychlorinated Biphenyls -- 2.6 Phenol and Derivatives -- 3...Evaluation of Organic Toxicant-Induced Health Risks Using Eukaryotic Cell-Based Bioluminescent Assays -- 3.1 Bioassays for Dioxin and Dioxin-Like Compounds -- 3.2 Bioassays for Hormonally Active Chemicals -- 3.3 Environmental Applications -- 4...Conclusions -- References -- Part IIIApplications of Bioluminescencein Agriculture and Bioprocess -- 5 Detection of Bacteria with Bioluminescent Reporter Bacteriophage -- Abstract -- 1...Introduction -- 2...Bioluminescence as Reporter System -- 3...Principles of Reporter Bacteriophage Construction and Use -- 4...Specific Bioluminescent Reporter Phages -- 4.1 Antibiotic Resistance -- 4.2 Combination of Bioluminescent Reporter Phages with Other Technologies -- 5...Summary, Conclusions, and Outlook.

References -- Part IVApplications of Bioluminescencein Health -- 6 Application of Enzyme Bioluminescence for Medical Diagnostics -- Abstract -- 1...Introduction -- 2...Immunoassay: State-of-the-Art -- 2.1 Luciferases as Labels for Immunoassay -- 3...Nucleic Acid Hybridization Assay Based on Bioluminescent Reporters -- 3.1 Bioluminescent Assay of Infectious Agents -- 3.2 Bioluminescent Assay in Oncology Diagnostics -- 4...Multianalytical Bioluminescent Assay -- 5...Bioluminescent Binding Assay in Homogeneous Format -- 6...Summary, Conclusions, and Outlook -- References -- Index.

Sommario/riassunto

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however



always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.