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Record Nr. |
UNINA9910298329003321 |
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Titolo |
Fluorescent Methods for Molecular Motors / / edited by Christopher P. Toseland, Natalia Fili |
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Pubbl/distr/stampa |
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Basel : , : Springer Basel : , : Imprint : Springer, , 2014 |
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ISBN |
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Edizione |
[1st ed. 2014.] |
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Descrizione fisica |
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1 online resource (306 p.) |
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Collana |
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Experientia Supplementum, , 1664-431X ; ; 105 |
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Disciplina |
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Soggetti |
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Proteins |
Cell biology |
Biophysics |
Biological physics |
Protein Science |
Cell Biology |
Biological and Medical Physics, Biophysics |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references at the end of each chapters. |
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Nota di contenuto |
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Fluorescence and Labelling: How to choose and what to do -- Fluorescent biosensors: design and application to motor proteins -- Rapid Reaction Kinetic Techniques -- Fluorescence to study the ATPase mechanism of Motor Proteins -- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies -- Fluorescent methods to study transcription initiation and transition into elongation -- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo -- Fluorescence methods in the investigation of the DEAD-box helicase mechanism -- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins -- Fluorescence Tracking of Motor proteins in vitro -- Measuring Transport of Motor Cargos -- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET -- Using fluorescence to study actomyosin in yeasts. |
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Sommario/riassunto |
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This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is |
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