Transgenic insects : techniques and applications / / Mark Q. Benedict, editor

• Record Nr.: UNINA9910284952203321
• Pubbl/distr/stampa: Oxfordshire, England ; ; Boston, Massachusetts : , : CABI, , 2014; ©2014
• ISBN: 1-78924-463-3; 1-78064-452-3
• Descrizione fisica: 1 online resource (401 p.)
• Collana: CABI Biotechnology Series ; ; 3
• Disciplina: 631.5233
• Soggetti: Insects - Genetics; Transgenic animals; Insect pests - Control
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references at the end of each chapters and index.
• Nota di contenuto: Contents; Contributors; Acknowledgements; Preface; PART 1: GERMLINE TRANSFORMATION TECHNOLOGY; 1 Transposons for Insect Transformation; 1.1 Transposable Elements; 1.2 DNA Transposons; 1.3 Transposons with Activity in Insects; 1.4 Summary; References; 2 Transposon-Based Technologies for Insects; 2.1 Transposon-Based Technologies; 2.2 Mutagenesis; 2.3 Germ-Line Transformation; 2.4 Modular Expression Systems; 2.5 Cell/Genetic Ablation; 2.6 Transgene Mis-expression; 2.7 Transgenic Gene Silencing; 2.8 Site-Specifi c Recombination; 2.9 Genetic Sensors; 2.10 Conclusions; References; 3 Sex-, Tissue- and Stage-Specifi c Transgene Expression3.1 Introduction; 3.2 Gene Regulation in Insects; 3.3 Th e Basic Genetic Construct; 3.4 Controlling for Position Eff ects; 3.5 General Considerations for Promoter Choice; 3.6 Sex-Specifi c Transgene Expression; 3.7 Tissue-Specifi c Expression; 3.8 Stage-Specifi c Expression; 3.9 Future Prospects; 3.10 Applications of Sex-, Tissue- and Stage-Specifi c Transgene Expression; References; 4 Docking Systems for Site-Directed Transgene Integration; 4.1 Background and Introduction; 4.2 Site-Specifi c Transgenesis - Generation of Phase 1 Docking Strains4.3 Site-Specifi c Transgenesis - Generation of Phase 2 Integrations; 4.4 Recombinase-Mediated Cassette Exchange; 4.5 Future Developments in the Use of Docking Systems; 4.6 Docking Systems Combined with Transposon Stabilization Strategies; 4.7 Integration of Large, Complex Transgene Constructs; 4.8 Construction of Complex Transgenes by Sequential Use of Integrases; References; 5 Inducible and Repressible Systems for Transgene Expression; 5.1 Introduction; 5.2 Naturally Occurring Systems of Conditional Expression; 5.3 Synthetic Systems5.4 Conclusions; References; 6 Sex Ratio Manipulation for Insect Population Control; 6.1 Introduction; 6.2 Overview of Applications and General Principles; 6.3 Meiotic Drive; 6.4 Sex-Specifi c Lethality; 6.5 Manipulation of Sex Determination Mechanisms; 6.6 Conclusions; References; 7 Conditional Dominant Lethals - RIDL; 7.1 Re-engineering the Sterile Insect Technique; 7.2 Sterile Insects and Genetic Control; 7.3 Engineered Traits; 7.4 Integrated Pest Management; 7.5 Resistance Management; 7.6 Molecular Designs; 7.7 Choosing an Eff ector; 7.8 Choice of Switch; 7.9 Strain Performance7.10 Penetrance; 7.11 Resistance; 7.12 Field Experience and Future Prospects; Notes; Acknowledgements; References; PART 2: APPLICATIONS OF TRANSGENIC INSECTS; 8 Tephritid Fruit Fly Transgenesis and Applications; 8.1 Introduction; 8.2 Transformation with the Minos Vector System; 8.3 Transformation with the piggyBac Vector System; 8.4 Transformation with the Hermes Vector System; 8.5 Marker Systems for Transformant Organismal and Tissue Detection in Tephritid Flies; 8.6 Post-integration Stabilization of Transposon Vectors in Tephritid Flies; 8.7 Site-Specifi c Genomic Targeting in Tephritids
• Sommario/riassunto: Insect transgenesis promises improvements in agriculture, pharmaceuticals and public health. Many important insects can now be routinely transformed with effectors that have useful applications. Agriculture presents the largest market for transgenic insects and has a foundational history of success with sterile insect technique for control of pests including Mediterranean fruit flies and screwworms. Biotechnology will contribute superior markers, suppressible sterility and sex-conversion. Public health is also seeing transgenic mosquitoes developed which suppress natural populations and are in