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Record Nr. |
UNINA9910254147703321 |
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Autore |
Terasaka Naohiro |
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Titolo |
Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA / / by Naohiro Terasaka |
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Pubbl/distr/stampa |
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Tokyo : , : Springer Japan : , : Imprint : Springer, , 2017 |
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ISBN |
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9784431565154 |
9784431565178 (e-book) |
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Edizione |
[1st ed. 2017.] |
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Descrizione fisica |
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1 online resource (XII, 89 p. 38 illus., 28 illus. in color.) |
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Collana |
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Springer Theses, Recognizing Outstanding Ph.D. Research, , 2190-5053 |
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Disciplina |
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Soggetti |
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Bioorganic chemistry |
Catalysis |
Nucleic acids |
Biotechnology |
Bioorganic Chemistry |
Nucleic Acid Chemistry |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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"Doctoral Thesis accepted by the University of Tokyo, Tokyo, Japan." |
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Nota di bibliografia |
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Includes bibliographical references. |
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Nota di contenuto |
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General introduction -- Discovery of human microRNA precursor binding to folic acid by small RNA transcriptomic SELEX -- Orthogonal ribosome-tRNAs pair by engineering of peptidyl transferase center -- General conclusion. |
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Sommario/riassunto |
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In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to |
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