1.

Record Nr.

UNINA9910156338203321

Titolo

Microarray Bioprinting Technology : Fundamentals and Practices / / edited by Moo-Yeal Lee

Pubbl/distr/stampa

Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016

ISBN

9783319468051

Edizione

[1st ed. 2016.]

Descrizione fisica

1 online resource (XIII, 175 p. 120 illus., 114 illus. in color.)

Disciplina

612.028

571.538

Soggetti

Regenerative medicine

Tissue engineering

Biomedical engineering

Biotechnology

Regenerative Medicine/Tissue Engineering

Biomedical Engineering and Bioengineering

Microengineering

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Nota di bibliografia

Includes bibliographical references at the end of each chapters.

Nota di contenuto

1. Overview of Microarray Bioprinting Technology -- 2. Microarray Spotter and Printing Technologies -- 3. Chip Platforms and Chip Surface Treatments -- 4. Biological Sample Printing -- 5. High Content Cell Staining -- 6. 3D-Cultured Cell Image Acquisition -- 7. High-Content Image Analysis -- 8. Applications of Microarray Bioprinting.

Sommario/riassunto

This book introduces key fundamentals of microarray bioprinting, including the required chip platforms and associated instruments/devices, experimental protocols for cell printing and biochemical- and cell-based assays, and several example applications. Various bioprinting approaches that allow for the rapid testing of hundreds of different cell culture conditions in combinations on a single chip are discussed in detail. Also covered is high-content, 3D cell-based imaging assays of tissue functions on miniaturized tissue constructs for high-throughput, predictive screening of drug efficacy and toxicity. This is an ideal book for graduate and postgraduate



students in the field of biomedical engineering as well as scientists in the pharmaceutical industry. This book also: Broadens readers’ understanding of the principles of microarray bioprinting, chip platforms and associated instruments/devices, and surface chemistry for micropatterning of cells on the chip platform Covers the latest developments in printing cells in hydrogels and methods of gelation as well as printing other biological samples in aqueous solutions Illustrates the complete process for cell staining and high-content imaging of 3D cells on the chip and predicting human metabolism and toxicology on the chip.