1.

Record Nr.

UNISA996318448103316

Autore

Schiesser Alexandra

Titolo

Dialekte machen : Konstruktion und Gebrauch arealer Varianten im Kontext sprachraumbezogener Alltagsdiskurse / / Alexandra Schiesser

Pubbl/distr/stampa

Berlin/Boston, : De Gruyter, 2020

Berlin ; ; Boston : , : De Gruyter, , [2019]

©2020

ISBN

3-11-066028-8

3-11-066023-7

Descrizione fisica

1 online resource (429)

Collana

Linguistik - Impulse & Tendenzen ; ; 85

Disciplina

437/.9494

Soggetti

Linguistics

Sociolinguistics

Dialect, slang & jargon

Switzerland

Lingua di pubblicazione

Tedesco

Formato

Materiale a stampa

Livello bibliografico

Monografia

Nota di contenuto

Frontmatter -- Vorwort -- Abbildungsverzeichnis -- Tabellenverzeichnis -- Verzeichnis der Abkürzungen -- Inhalt -- 1. Einführung in die Thematik -- 2. Vom Interesse daran, wie Laien Sprache wahrnehmen -- 3. Sprache und Raum -- 4. Sprechen über Sprache -- 5. Sprachliche Variation -- 6. Stil -- 7. Identität -- 8. Erhebung der Daten -- 9. Aufbereitung der Daten -- 10. Mentale Strukturierung des Sprachraums -- 11. Diskursive Konstituierung des Sprachraums -- 12. Konzeptualisierung soziosymbolisch relevanter Varianten -- 13. Gruppenspezifischer Gebrauch soziosymbolisch relevanter Varianten -- 14. Individueller Gebrauch soziosymbolisch relevanter Varianten -- 15. Zusammenschau der Ergebnisse -- 16. Ausblick -- Literaturverzeichnis -- Register

Sommario/riassunto

Die empirische Studie untersucht die gesellschaftliche Bedeutung von Dialekt und Dialektgebrauch in der Schweiz. Über interdisziplinäre Zugänge arbeitet sie heraus, wie (eng) laienlinguistische Wahrnehmung und Bewertung von Sprache mit dem Sprachgebrauch in Zusammenhang stehen. Sie zeigt, dass Dialekte über diskursive



Prozesse konstruiert und im Sprachgebrauch reproduziert werden und damit, wie Dialekte von Sprechern gemacht werden.

This empirical study examines the importance of dialect and dialect use in Switzerland. Based on interdisciplinary approaches, it explores the extent to which linguistic perception and assessment on the part of laypersons is connected to actual language use. It shows that dialects are constructed by means of discursive processes and reproduced in language use. In this way, it illuminates how speakers create dialects.

2.

Record Nr.

UNINA9910155326503321

Autore

Velmurugu Yogambigai

Titolo

Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition / / by Yogambigai Velmurugu

Pubbl/distr/stampa

Cham : , : Springer International Publishing : , : Imprint : Springer, , 2017

ISBN

9783319451299

Edizione

[1st ed. 2017.]

Descrizione fisica

1 online resource (XXI, 199 p. 112 illus., 105 illus. in color.)

Collana

Springer Theses, Recognizing Outstanding Ph.D. Research, , 2190-5053

Disciplina

571.4

Soggetti

Biophysics

Spectrum analysis

Microscopy

Proteins

Biological and Medical Physics, Biophysics

Spectroscopy and Microscopy

Protein-Ligand Interactions

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Nota di bibliografia

Includes bibliographical references.

Nota di contenuto

Introduction -- Methods -- Integration Host Factor (IHF)-DNA interaction -- Lesion Recognition by Xeroderma Pigmentosum C (XPC) Protein -- DNA Mismatch Repair.

Sommario/riassunto

Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this



study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins. Many cellular processes involve special proteins that bind to specific DNA sites with high affinity. How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific recognition. However, the dynamics of DNA deformations have been difficult to capture, thus obscuring our understanding of recognition mechanisms. The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually “stumbles” upon its target site. Submillisecond interrogation may promote preferential stalling of the rapidly scanning protein at cognate sites, thus enabling site-recognition. Such multi-step search-interrogation-recognition processes through dynamic conformational changes may well be common to the recognition mechanisms for diverse DNA-binding proteins. .