1.

Record Nr.

UNINA9910151563503321

Autore

Schuck Peter <1965, >

Titolo

Sedimentation velocity analytical ultracentrifugation : discrete species and size-distributions of macromolecules and particles / / Peter Schuck

Pubbl/distr/stampa

Boca Raton, Fla. : , : CRC Press, Taylor & Francis Group, , [2016]

©2016

ISBN

1-315-35012-2

0-367-87828-3

1-315-36723-8

Edizione

[1st ed.]

Descrizione fisica

1 online resource (267 pages)

Disciplina

572

Soggetti

Ultracentrifugation

Macromolecules - Analysis

Nanoparticles - Analysis

Sedimentation analysis

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Chapter 1. Basic analysis principles -- Chapter 2. Sedimentation of discrete non-interacting particles -- Chapter 3. Properties of sedimentation coefficient distributions -- Chapter 4. Distributions of non-diffusing particles -- Chapter 5. Distributions of diffusing particles -- Chapter 6. Sedimentation coefficient distributions from boundary derivatives and extrapolations -- Chapter 7. Multi-component distributions -- Chapter 8. Practical analysis of non-interacting systems.

Sommario/riassunto

The analysis of sedimentation velocity analytical ultracentrifugation has fundamentally changed in the last two decades, with the ability to solve the master equation of the sedimentation/diffusion process and the application of modern mathematical data analysis strategies substantially increasing hydrodynamic resolution of sedimenting particles. This book provides a systematic introduction to the theory of sedimentation velocity, and a comprehensive overview of experimental design strategies and data analysis. Areas of major focus are polydisperse macromolecules and nanoparticles, and the sedimentation



of systems of reversibly interacting macromolecules, including protein interactions--