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Record Nr. |
UNINA9910149172603321 |
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Autore |
Weinberger Robert |
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Titolo |
Practical capillary electrophoresis / / Robert Weinberger |
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Pubbl/distr/stampa |
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San diego, California : , : Academic Press, , 2000 |
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©2000 |
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ISBN |
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Edizione |
[2nd ed.] |
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Descrizione fisica |
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1 online resource (915 p.) |
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Disciplina |
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Soggetti |
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Capillary electrophoresis |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references at the end of each chapters and index. |
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Nota di contenuto |
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Cover image; Title page; Table of Contents; Copyright; Dedication; PREFACE TO THE SECOND EDITION; PREFACE TO THE FIRST EDITION; MASTER SYMBOL LIST; Chapter 1: Introduction; 1.1 ELECTROPHORESIS; 1.2 MICROCHROMATOGRAPHIC SEPARATION METHODS; 1.3 CAPILLARY ELECTROPHORESIS; 1.4 CAPILLARY ELECTROCHROMATOGRAPHY; 1.5 MICROMACHINED ELECTROPHORETIC DEVICES; 1.6 HISTORICAL PERSPECTIVE; 1.7 GENERIC HPCE SYSTEMS; 1.8 INSTRUMENTATION; 1.9 SOURCES OF INFORMATION ON HPCE; 1.10 CAPILLARY ELECTROPHORESIS: A FAMILY OF TECHNIQUES; Chapter 2: Capillary Zone Electrophoresis: Basic Concepts |
2.1 ELECTRICAL CONDUCTION IN FLUID SOLUTION2.2 THE LANGUAGE OF ELECTROPHORESIS; 2.3 ELECTROENDOOSMOSIS; 2.4 EFFICIENCY; 2.5 RESOLUTION; 2.6 JOULE HEATING; 2.7 OPTIMIZING THE VOLTAGE AND TEMPERATURE; 2.8 CAPILLARY DIAMETER AND BUFFER IONIC STRENGTH; 2.9 OPTIMIZING THE CAPILLARY LENGTH; 2.10 BUFFERS; 2.11 TEMPERATURE EFFECTS; 2.12 BUFFER ADDITIVES; 2.13 CAPILLARIES; 2.14 SOURCES OF BAND BROADENING; Chapter 3: Capillary Zone Electrophoresis: Methods Development; 3.1 INTRODUCTION; 3.2 MOBILITY; 3.3 SOLUTE-WALL INTERACTIONS; 3.4 SEPARATION STRATEGIES; 3.5 SECONDARY EQUILIBRIUM |
3.6 APPLICATIONS AND TECHNIQUESChapter 4: Capillary Zone Electrophoresis: Secondary Equilibrium, Micelles, Cyclodextrins, and Related Reagents; 4.1 INTRODUCTION; 4.2 MICELLES; 4.3 SEPARATION |
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MECHANISM; 4.4 SELECTING THE ELECTROLYTE SYSTEM; 4.6 ALTERNATIVE SURFACTANT SYSTEMS; 4.7 CYCLODEXTRINS; 4.8 APPLICATIONS AND METHODS DEVELOPMENT; 4.9 CHIRAL RECOGNITION; 4.10 AFFINITY CAPILLARY ELECTROPHORESIS; Chapter 5: Capillary Isoelectric Focusing; 5.1 BASIC CONCEPTS; 5.2 SEPARATION MECHANISM; 5.3 pH GRADIENT FORMATION; 5.4 ELECTRODE BUFFER SOLUTIONS; 5.5 RESOLVING POWER; 5.6 CAPILLARIES AND REAGENTS |
5.7 PERFORMING A RUN5.8 INJECTION; 5.9 FOCUSING; 5.10 MOBILIZATION; 5.11 SALT EFFECTS; 5.12 DETECTION; 5.13 APPLICATIONS; Chapter 6: Size Separations in Capillary Gels and Polymer Networks; 6.1 INTRODUCTION; 6.2 SEPARATION MECHANISM; 6.3 MATERIALS FOR SIZE SEPARATIONS; 6.4 SIZE SEPARATIONS WITH NONREPLACEABLE POLYACRYLAMIDE; 6.5 SIZE SEPARATIONS WITH REPLACEABLE AGAROSE; 6.6 INTRODUCTION TO POLYMER NETWORKS; 6.7 OPERATING CHARACTERISTICS OF POLYMER NETWORKS; 6.8 ADDITIONAL MATERIALS FOR POLYMER NETWORKS; 6.9 DETECTION; 6.10 OPERATING HINTS USING POLYMER NETWORKS |
6.11 APPLICATIONS AND METHODS DEVELOPMENT6.12 REDUCING THE PROBLEM OF BIASED REPTATION; Chapter 7: Capillary Electrochromatography; 7.1 INTRODUCTION; 7.2 MODES OF CEC; 7.3 ELECTROOSMOTIC FLOW IN CEC; 7.4 EFFICIENCY OF CEC; 7.5 OPERATING CHARACTERISTICS OF PACKED CEC; 7.6 APPLICATIONS; 7.7 CEC MICROFLUIDIC DEVICES; Chapter 8: Injection; 8.1 VOLUMETRIC CONSTRAINTS ON INJECTION SIZE; 8.2 PERFORMING AN INJECTION AND A RUN; 8.3 INJECTION TECHNIQUES; 8.4 SHORT-END INJECTION; 8.5 INJECTION ARTIFACTS: PROBLEMS AND SOLUTIONS; 8.6 STACKING AND TRACE ENRICHMENT; Chapter 9: Detection |
9.1 ON-CAPILLARY DETECTION |
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Sommario/riassunto |
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In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small mole |
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