China since Tiananmen : the politics of transition / Joseph Fewsmith

• Record Nr.: UNINA990007447610403321
• Autore: Fewsmith, Joseph
• Pubbl/distr/stampa: Cambridge : Cambridge University Press, 2001
• ISBN: 0-521-00105-6
• Descrizione fisica: XVII, 313 p. ; 24 cm
• Collana: Cambridge modern China series
• Disciplina: 951.059
• Locazione: FSPBC
• Collocazione: XIII A 248
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia

Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen

• Record Nr.: UNINA9910146242103321
• Pubbl/distr/stampa: Weinheim ; ; New York, : Wiley-VCH, c2003
• ISBN: 1-280-52042-6; 9786610520428; 3-527-60533-9; 3-527-60149-X
• Descrizione fisica: 1 online resource (984 p.)
• Altri autori (Persone): LorkowskiStefan; CullenPaul, Dr.
• Disciplina: 572.8/65; 572.865
• Soggetti: Gene expression; Electronic books.
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Analysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters; 1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes; 1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination; 1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 β-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification; 1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation; 2.2.3 Surface affinity chromatography
• Sommario/riassunto: This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp ; the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying parti