1.

Record Nr.

UNINA9910451694003321

Titolo

Biological low-voltage scanning electron microscopy [[electronic resource] /] / edited by Heide Schatten, James B. Pawley

Pubbl/distr/stampa

New York, : Springer, c2008

ISBN

1-281-13990-4

9786611139902

0-387-72972-0

Edizione

[1st ed. 2008.]

Descrizione fisica

1 online resource (328 p.)

Altri autori (Persone)

SchattenHeide

PawleyJames B

Disciplina

570.2825

Soggetti

Low-voltage scanning electron microscopy

Field emission cathodes

Scanning electron microscopes

Electronic books.

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

The Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy.

Sommario/riassunto

Field-emission, low-voltage scanning electron microscopy (LVSEM) is a field that has grown tremendously in recent years because is offers the optimal method for viewing complex surfaces at high resolution and in three dimensions. However, even though the instrumentation required to get good results at low beam voltage has become increasingly



available, there has been a lag in its application to biological specimens. What seemed to be missing was volume that combined both the theory and practice of using this equipment in an optimal manner with a thorough treatment of biological specimen preparation. Biological Low-Voltage Scanning Electron Microscopy is the first book to address both of these aspects of biological LVSEM. After providing a thorough description of the unique advantages and the operating constraints related to operating a scanning electron microscope at low beam voltage, the remainder of book focuses on the the best way to image all types of plant and animal cells and covers specimens that range from macromolecules to the surfaces revealed by de-embedding resin-embedded samples. Advanced specimen preparation techniques such as cryo-LVSEM, and immuno-gold-LVSEM are fully covered, as is x-ray microanalysis at low beam voltage and live-time stereo imaging. The preparative protocols provided represent the distilled essence of the experience of a group of world-renowned authors who have, for many decades, been instrumental in developing and applying new approaches to LVSEM to support their own biological research.

2.

Record Nr.

UNINA9910146147703321

Autore

Gonzalez Catherine

Titolo

Rapid chemical and biological techniques for water monitoring [[electronic resource] /] / Catherine Gonzalez, Richard Greenwood, Philippe Quevauviller

Pubbl/distr/stampa

Chichester, West Sussex, U.K. ; ; Hoboken, NJ, : Wiley, 2009

ISBN

1-282-12365-3

9786612123658

0-470-74542-8

0-470-74543-6

Descrizione fisica

1 online resource (443 p.)

Collana

Water quality measurements series

Altri autori (Persone)

GreenwoodR (Richard)

QuevauvillerPh

Disciplina

628.1

628.1/61

628.161

Soggetti

Water quality bioassay

Environmental monitoring

Water - Pollution

Water - Microbiology

Water chemistry

Chemistry, Analytic



Electronic books.

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Rapid Chemical and Biological Techniques for Water Monitoring; Contents; Series Preface; Preface; The Series Editor - Philippe Quevauviller; List of Contributors; Section 1 Screening Methods in the Context of Water Policies; 1.1 WFD Monitoring and Metrological Implications; 1.2 Use of Screening Methods in US Water Regulation; 1.3 Existing and New Methods for Chemical and Ecological Status Monitoring under the WFD; Section 2 Chemical Methods; 2.1 The Potential of Passive Sampling to Support Regulatory Monitoring of the Chemical Quality of Environmental Waters

2.2 Polar Organic Chemical Integrative Sampler and Semi-permeable Membrane Devices2.3 Main Existing Methods for Chemical Monitoring; 2.4 UV Spectrophotometry: Environmental Monitoring Solutions; Section 3 Biological Methods; 3.1 Application of Microbial Assay for Risk Assessment (MARA) to Evaluate Toxicity of Chemicals and Environmental Samples; 3.2 Bioassays and Biosensors; 3.3 Immunochemical Methods; 3.4 Biomolecular Recognition Systems for Water Monitoring; 3.5 Continuous Monitoring of Waters by Biological Early Warning Systems

3.6 Biological Markers of Exposure and Effect for Water Pollution MonitoringSection 4 Potential Use of Screening Methods and Performance Evaluation; 4.1 Monitoring Heavy Metals Using Passive Sampling Devices; 4.2 On-site Heavy Metal Monitoring Using a Portable Screen-printed Electrode Sensor; 4.3 Field Monitoring of PAHs in River Water by Direct Fluorimetry on C18 Solid Sorbent; 4.4 Evaluation of the Field Performance of Emerging Water Quality Monitoring Tools; 4.5 Sampling Uncertainty and Environmental Variability for Trace Elements on the Meuse River, France

Section 5 Quality Assurance and Validation Method5.1 Preparation of Reference Materials for Proficiency Testing Schemes; 5.2 Participation of Screening Methods and Emerging Tools (SMETs) to Proficiency Testing Schemes on the Determination of Priority Substances in Real Water Matrices Organized in Support of the Water Framework Directive Implementation; 5.3 Traceability and Interlaboratory Studies on Yeast-based Assays for the Determination of Estrogenicity; Section 6 Integration of Screening Methods in Water Monitoring Strategies

6.1 Assessing the Impacts of Alternative Monitoring Methods and Tools on Costs and Decision Making: Methodology and Experience from Case Studies6.2 Acceptance of Screening Methods by Actors Involved in Water Monitoring; Index

Sommario/riassunto

Rapid Chemical and Biological Techniques for Water Monitoring presents in one volume the broad spectrum of monitoring tools, both available and under development, and provides an assessment of their potential for underpinning environmental management and legislation. The book explores screening methods in the context of water policies; chemical methods; biological methods; potential use of screening methods; quality assurance and validation methods; integration of screening methods in water monitoring strategies. The text provides a



timely source of information for post-graduates, resea