| |
|
|
|
|
|
|
|
|
1. |
Record Nr. |
UNINA9910146071003321 |
|
|
Autore |
Kinter Michael |
|
|
Titolo |
Protein sequencing and identification using tandem mass spectrometry [[electronic resource] /] / Michael Kinter, Nicholas E. Sherman |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
New York, : Wiley-Interscience, c2000 |
|
|
|
|
|
|
|
ISBN |
|
1-280-54166-0 |
9786610541669 |
0-471-23188-6 |
0-471-72198-0 |
|
|
|
|
|
|
|
|
Descrizione fisica |
|
1 online resource (321 p.) |
|
|
|
|
|
|
Collana |
|
Wiley-Interscience series on mass spectrometry |
|
|
|
|
|
|
Altri autori (Persone) |
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
Soggetti |
|
Nucleotide sequence |
Proteins |
Mass spectrometry |
|
|
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Note generali |
|
Description based upon print version of record. |
|
|
|
|
|
|
Nota di bibliografia |
|
Includes bibliographical references and index. |
|
|
|
|
|
|
Nota di contenuto |
|
PROTEIN SEQUENCING AND IDENTIFICATION USING TANDEM MASS SPECTROMETRY; CONTENTS; Series Preface; Preface; Chapter 1. An Introduction to Protein Sequencing Using Tandem Mass Spectrometry; 1.1. Introduction; 1.2. References; Chapter 2. The Primary Structure of Proteins and a Historical Overview of Protein Sequencing; 2.1. Protein and Peptide Structure; 2.2. Edman Degradation; 2.2.1. The Edman Reaction; 2.2.2. Incorporation of the Edman Degradation Reaction into Automated Protein Sequenators; 2.2.3. Edman Degradation in Proteomic Research; 2.3. Tandem Mass Spectrometry |
2.3.1. A Brief History of the Application of Mass Spectrometry to Protein Sequencing2.3.2. Sequence Analysis of Peptides Using Electron Ionization Mass Spectrometry; 2.3.3. The Utilization of Fast Atom Bombardment with Tandem Mass Spectrometry to Sequence Peptides; 2.3.4. Internal Sequence Analysis of Proteins Using Electrospray Ionization-Tandem Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization-Time-of- Flight Mass Spectrometry; 2.4. Summary; 2.5. References; Chapter 3. Fundamental Mass Spectrometry; 3.1. An Overview of the Instrumentation; 3.2. Ionization Methods |
|
|
|
|
|
|
|
|
|
|
|
3.2.1. Electrospray Ionization3.2.2. Nanospray and Microspray Ionization; 3.2.3. Matrix-Assisted Laser Desorption/ Ionization; 3.3. Mass Analyzers; 3.3.1. Fundamental Parameters of Mass Analysis; 3.3.2. Quadrupole Mass Filters (3.20); 3.3.3. Ion Trap Mass Analyzers (3.21-3.23); 3.3.4. Time-of-Flight Mass Analyzers (3.5); 3.4. Tandem Mass Spectrometry; 3.4.1. Collisionally Induced Dissociation; 3.4.2. Tandem Mass Spectrometers; 3.4.3. Types of Tandem Mass Spectrometry Experiments; 3.5. Data Systems; 3.6. Summary; 3.7. References |
Chapter 4. Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra4.1. Introduction; 4.2. Peptide Fragmentation Chemistry; 4.2.1. Collisionally Induced Dissociation of Peptide Ions Formed by Electrospray Ionization; 4.2.2. Fragmentation of Protonated Peptide Ions Formed by Matrix-Assisted Laser Desorption/Ionization; 4.3. Interpretation of the Product Ion Spectra of Tryptic Peptides; 4.3.1. Tabulated Values Used in the Interpretation; 4.3.2. A Strategy for the Interpretation of Product Ion Spectra of Tryptic Peptides |
4.3.3. Sample Interpretation Problem Number One4.3.4. Sample Interpretation Problem Number Two; 4.3.5. A Summary of Interpretation Problems One and Two; 4.3.6. Examples of More Difficult Product Ion Spectra That Cannot Be Completely Interpreted; 4.3.7. Interpretation of Product Ion Spectra from Triply Charged Ions; 4.4. Summary; 4.5. References; Chapter 5. Basic Polyacrylamide Gel Electrophoresis; 5.1. Introduction; 5.2. The Principles of Gel Electrophoresis; 5.2.1. Protein Movement and Separation; 5.2.2. Protein Detection |
5.3. The Basic Steps in a Polyacrylamide Gel Electrophoresis Experiment |
|
|
|
|
|
|
Sommario/riassunto |
|
How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
2. |
Record Nr. |
UNINA9910815246503321 |
|
|
Autore |
Clément Daniel <1951-> |
|
|
Titolo |
The bungling host : the nature of Indigenous oral literature / / Daniel Clément ; translated by Peter Frost |
|
|
|
|
|
|
|
Pubbl/distr/stampa |
|
|
Lincoln ; ; London : , : University of Nebraska Press, , 2018 |
|
|
|
|
|
|
|
ISBN |
|
|
|
|
|
|
Descrizione fisica |
|
|
|
|
|
|
Classificazione |
|
|
|
|
|
|
Disciplina |
|
|
|
|
|
|
Soggetti |
|
Indian mythology |
Folklore - North America |
Indians of North America |
Animals - Symbolic aspects |
Ethnology - North America |
Montagnais Indians |
Innu Indians |
Myth |
SOCIAL SCIENCE / Ethnic Studies / Native American Studies |
LITERARY CRITICISM / Native American |
|
|
|
|
|
|
|
|
Lingua di pubblicazione |
|
|
|
|
|
|
Formato |
Materiale a stampa |
|
|
|
|
|
Livello bibliografico |
Monografia |
|
|
|
|
|
Nota di contenuto |
|
Machine generated contents note: Contents List of Illustrations Introduction 1. Caribou Takes In His Wife's Dress (Subarctic) 2. Snake Makes a Meal in the Embers (Southwest) 3. The Fire Trap (Grand Basin) 4. While Bird Sings, Bear Cooks (Northwest Coast) 5. Seal Roasts His Hands (Northwest Coast) 6. Silver Fox Digs Up Yellow Jacket Larvae with His Penis (California) 7. Wildcat Beats a Blanket (California) 8. Deer Kills Her Children and Puts Their Bones Into the Water (Southwest) 9. Wolf Transforms Two Arrowheads into Mincemeat Puddings (Southwest) 10. Badger Pushes a Stick Down His Throat and Gets Yucca-Juice (Southwest) 11. Bison Skewers His Nose (Plains) 12. White-Tailed Deer Shoots at a Red Clay Bank (Plains) 13. Man Kills Bison with His Sharpened Leg (Plains, Plateau) 14. Black-Mountain-Bear Gets Persimmons by Leaning Against a Tree (Southeast) 15. Rabbit Gathers |
|
|
|
|
|
|
|
|
|
|
|
Canes (Southeast) 16. Squirrel Slits Open His Scrotum (Plains) 17. Duck Excretes Rice (Northeast) 18. Bird Gets Salmon Eggs by Striking His Ankle (Northwest Coast) 19. Muskrat Cooks Some Ice (Northeast) 20. Woodpecker Pulls Eels Out of Trees (Subarctic) Conclusion Appendix: Bungling Host Myths Notes Bibliography. |
|
|
|
|
|
|
Sommario/riassunto |
|
"Daniel Clement examines the "Bungling Host" tale known in a multitude of indigenous cultures in North America and beyond. In this groundbreaking work he reveals fuller meaning to these stories than previously recognized and underscores the limits of structuralism in understanding them"-- |
"The Bungling Host motif appears in countless indigenous cultures in North America and beyond. In this groundbreaking work Daniel Clement has gathered more than four hundred North American variants of the story to examine how myths acquire meaning for their indigenous users and explores how seemingly absurd narratives can prove to be a rich source of meaning when understood within the appropriate context. In analyzing the Bungling Host tales, Clement considers not only material culture but also social, economic, and cultural life; Native knowledge of the environment; and the world of plants and animals.<BR /><BR /> Clement's analysis uncovers four operational modes in myth construction and clarifies the relationship between mythology and science. Ultimately he demonstrates how science may have developed out of an operational mode that already existed in the mythological mind.<BR />"-- |
|
|
|
|
|
|
|
| |