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Record Nr. |
UNINA9910144111403321 |
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Titolo |
Clinical proteomics : from diagnosis to therapy / / edited by Jennifer E. Van Eyk and Michael J. Dunn |
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Pubbl/distr/stampa |
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Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 |
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©2008 |
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ISBN |
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1-281-94674-5 |
9786611946746 |
3-527-62215-2 |
3-527-62216-0 |
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Descrizione fisica |
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1 online resource (695 p.) |
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Disciplina |
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Soggetti |
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Proteomics |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Nota di contenuto |
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Clinical Proteomics; Contents; Editor's Overview; Acknowledgements; List of Contributors; I Technologies; 1 Preanalytical Issues in Clinical Proteomic Studies; 1.1 Introduction; 1.2 Preanalytical Factors; 1.2.1 Biological Variation; 1.2.1.1 Intrinic Influences/Factors; 1.2.1.2 Extrinsic Influences/Factors; 1.2.2 Technical Variables; 1.2.2.1 Specimen/Sample Collection Mode; 1.2.2.2 Type of Sample Container; 1.2.2.3 Sample Processing and Handling Conditions; 1.2.2.4 Sample Storage; 1.3 Summary and Concluding Remarks; 2 Protein Separation by Two-Dimensional Electrophoresis; 2.1 Introduction |
2.2 2DE: Protein Solubilization and Sample Preparation2.3 2DE: Protein Separation; 2.3.1 Focusing in the First Dimension; 2.3.2 Advances in IEF; 2.4 Improving Proteomic Coverage by Subcellular Fractionation; 2.5 Protein Detection and Image Analysis; 2.6 The Future of 2DE; 3 Protein Separation: Liquid Chromatography; 3.1 Introduction; 3.2 Liquid Chromatography; 3.2.1 HPLC Separation Principles; 3.2.2 Reversed-Phase LC (RPLC, 1DLC); 3.2.3 Affinity Chromatography; 3.2.4 Size-Exclusion Chromatography; 3.2.5 Ion-Exchange Chromatography; 3.2.6 |
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Two-Dimensional LC |
3.2.6.1 Chromatofocusing to Reversed Phase3.2.6.2 Ion-Exchange-Reversed-Phase Liquid Chromatography; 3.2.7 Three-Dimensional Liquid Chromatography; 3.2.8 LC Image Analysis Requirement; 3.2.9 Mass Spectrometry for LC; 3.2.9.1 MALDI-TOF MS; 3.2.9.2 ESI-MS/MS; 3.3 Conclusions; 4 HPLC in Protein Discovery; 4.1 Introduction; 4.2 LC-Based Approaches in Peptide Mass Mapping; 4.3 LC-Based Approaches in Protein Mapping; 4.4 Orthogonal 2D HPLC Separations; 4.5 Conclusion; 5 IEF Analysis of Peptides for Biomarkers Discovery; 5.1 Introduction; 5.2 Background; 5.2.1 Isoelectric Focusing |
5.2.2 Shotgun Proteomics5.2.3 Shotgun IEF; 5.3 Shotgun IEF Workflow; 5.4 Applications; 5.5 Discussion and Outlook; 6 Capillary Electrophoretic Separations for Clinical Proteomics; 6.1 Introduction; 6.2 (Single-Dimension) Capillary Electophoretic Separation; 6.3 Capillary Electrophoresis-Based Multidimensional Separations; 6.3.1 Capillary Liquid Chromatography-Capillary Electrophoresis; 6.3.2 Capillary Electrophoresis-Capillary Electrophoresis; 6.3.3 Capillary Electrophoresis-Liquid Chromatography; 6.3.3.1 Characterization of Human Saliva Proteome; 6.3.3.2 Targeted Tissue Proteomics |
6.4 Conclusions7 Quantitative Proteomics Using Nano-LC with High Accuracy Mass Spectrometry; 7.1 Introduction; 7.2 Fundamentals of a High Mass Accuracy-Based LC-MS Approach; 7.3 Nano-LC-MS for Enhanced Sensitivity and Dynamic Range Coverage; 7.4 Further Developments for Increasing Proteomic Throughput; 7.5 Obtaining More Robust Quantitative Proteomic Measurements; 7.6 Summary and Perspective; 8 Antibody Microarrays for Protein and Glycan Detection; 8.1 Introduction; 8.2 Antibody Preparation and Microarray Production; 8.3 Sandwich Assays with Fluorescence Detection |
8.4 Antibody Microarrays with Lectin Detection |
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Sommario/riassunto |
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Unparalleled in its scope and depth, this book brings together proteomic approaches in diagnosis and treatment from all clinical fields, including clinical toxicology. The result is a new discipline in molecular medicine that will revolutionize the treatment and prevention of cancer, stroke and other severe diseases.Following an overview of clinical proteomics, the authors look at the technologies available, before moving on to cancer, cardiopulmonary disease, diabetes and stroke. A whole section is devoted to toxicity and the work is rounded off with a discussion of the future of clinical |
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2. |
Record Nr. |
UNINA9910793423303321 |
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Autore |
Abrahms-Kavunenko Saskia |
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Titolo |
Enlightenment and the gasping city : Mongolian Buddhism at a time of environmental disarray / / Saskia Abrahms-Kavunenko |
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Pubbl/distr/stampa |
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Ithaca, New York ; ; London : , : Cornell University Press, , [2019] |
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©2019 |
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ISBN |
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1-5017-3765-1 |
1-5017-3766-X |
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Descrizione fisica |
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1 online resource (251 pages) |
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Disciplina |
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Soggetti |
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Buddhism - Mongolia - Ulaanbaatar |
Air - Pollution - Mongolia - Ulaanbaatar |
Human ecology - Mongolia - Ulaanbaatar |
Human ecology - Religious aspects - Buddhism |
Ethnology - Mongolia - Ulaanbaatar |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Front matter -- Contents -- Acknowledgments -- Note on Transliteration and Terms -- Introduction -- 1. Dust and Obscuration in a New Economy -- 2. A History of Enlightenment in Mongolia -- 3. Buddhism, Purification, and the Nation -- 4. Ignorance and Blur -- 5. Networks and Visibility -- 6. Karma and Purification -- 7. Removing Blockages, Increasing Energy -- 8. Temple Critiques -- 9. White Foods, Purification, and Enlightenment -- Conclusion: Stillness and Movement -- Glossary -- Notes -- References -- Index |
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Sommario/riassunto |
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With air pollution now intimately affecting every resident of Ulaanbaatar, the capital of Mongolia, Saskia Abrahms-Kavunenko seeks to understand how, as a physical constant throughout the winter months, the murky and obscuring nature of air pollution has become an active part of Mongolian religious and ritual life. Enlightenment and the Gasping City identifies air pollution as a boundary between the physical and the immaterial, showing how air pollution impresses itself on the urban environment as stagnation and blur. She explores how air pollution and related phenomena exist in dynamic tension with |
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Buddhist ideas and practices concerning purification, revitalisation and enlightenment. By focusing on light, its intersections and its oppositions, she illuminates Buddhist practices and beliefs as they interact with the pressing urban issues of air pollution, post-socialist economic vacillations, urban development, nationalism, and climate change. |
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