Dynamic studies in biology [[electronic resource] ] : phototriggers, photoswitches and caged biomolecules / / edited by Maurice Goeldner, Richard Givens

• Record Nr.: UNINA9910143965203321
• Pubbl/distr/stampa: Weinheim, : Wiley-VCH, c2005
• ISBN: 1-280-52060-4; 9786610520602; 3-527-60559-2; 3-527-60554-1
• Descrizione fisica: 1 online resource (587 p.)
• Altri autori (Persone): GoeldnerMaurice; GivensRichard
• Disciplina: 547.2
• Soggetti: Photobiochemistry; Biomolecules - Separation; Protective groups (Chemistry); Electronic books.
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Dynamic Studies in Biology Phototriggers, Photoswitches and Caged Biomolecules; Contents; Foreword; Preface; List of Authors; 1 Photoremovable Protecting Groups Used for the Caging of Biomolecules; 1.1 2-Nitrobenzyl and 7-Nitroindoline Derivatives; 1.1.1 Introduction; 1.1.1.1 Preamble and Scope of the Review; 1.1.1.2 Historical Perspective; 1.1.2 Synthetic Considerations; 1.1.3 Survey of Individual Caged Compounds and Caging Groups; 1.1.3.1 2-Nitrobenzyl Cages; 1.1.3.1.1 Mechanistic Aspects of Photocleavage and By-Product Reactions of 2-Nitrobenzyl Cages; 1.1.3.1.2 Representative Survey of Nitrobenzyl-Caged Compounds1.1.3.2 7-Nitroindoline Cages; 1.1.3.2.1 Mechanistic and Structural Aspects of Photochemical Cleavage of 1-Acyl-7-nitroindolines; 1.1.3.2.2 Survey of 7-Nitroindoline Caged Compounds; 1.1.4 Conclusion; References; 1.2 Coumarin-4-ylmethyl Phototriggers; 1.2.1 Introduction; 1.2.2 Spectroscopic and Photochemical Properties; 1.2.2.1 ; Overview; 1.2.2.2 Phototriggers; 1.2.2.2.1 MCM Groups: 7-Alkoxy-Substituted Coumarins; 1.2.2.2.2 DMCM Groups: 6,7-Dialkoxy-Substituted Coumarins; 1.2.2.2.3 Bhc Groups: 6-Bromo-7-alkoxy-Substituted Coumarins; 1.2.2.2.4 DEACM Groups: 7-Dialkylamino-Substituted Coumarins1.2.2.3 Target Molecules; 1.2.2.3.1 Phosphates; 1.2.2.3.2 Carboxylates and Sulfates; 1.2.2.3.3 Amines; 1.2.2.3.4 Alcohols and Phenols; 1.2.2.3.5 Carbonyl Compounds; 1.2.3 Synthesis; 1.2.3.1 Synthesis of Precursor Molecules (Caging Agents); 1.2.3.1.1 Phosphates; 1.2.3.1.2 Carboxylates; 1.2.3.1.3 Amines; 1.2.3.1.4 Alcohols and Phenols; 1.2.4 Applications; 1.2.5 Conclusion and Perspective; References; 1.3 p-Hydroxyphenacyl: a Photoremovable Protecting Group for Caging Bioactive Substrates; 1.3.1 Introduction and History; 1.3.2 p-Hydroxyphenacyl1.3.2.1 General Physical and Spectroscopic Properties; 1.3.2.2 Synthesis of pHP-Caged Substrates; 1.3.3 Mechanistic Studies; 1.3.3.1 A Triplet "Photo-Favorskii" Rearrangement; 1.3.3.2 Role of the Triplet Phenol; 1.3.3.3 Correlation of the (3)pK(a) with the Quantum Efficiency; 1.3.4 Applications; 1.3.4.1 Neurotransmitter Release; 1.3.4.2 Peptide Release; 1.3.4.3 Nucleotide Release; 1.3.4.4 Enzyme Photoswitches; 1.3.5 Advantages and Limitations; References; 1.4 Caging of ATP and Glutamate: a Comparative Analysis; 1.4.1 Introduction; 1.4.2 General Properties for Caging Groups1.4.3 Caged-ATP; 1.4.3.1 Introduction; 1.4.3.2 Syntheses of the P(3)-caged ATP Derivatives; 1.4.3.3 General Properties of the Caged-ATP Molecules; 1.4.3.4 Conclusion; 1.4.4 Caged Glutamate; 1.4.4.1 Introduction; 1.4.4.2 Syntheses of the Caged Glutamate Derivatives; 1.4.4.3 General Properties of the Caged-Glutamate Molecules; 1.4.4.4 Conclusion; Abbreviations; References; 2 Mechanistic Overview of Phototriggers and Cage Release; 2.1 Introduction; 2.2 Major Photoremovable Protecting Groups; 2.2.1 2-Nitrobenzyl (2-NB); 2.2.1.1 The Reactive Excited State; 2.2.1.2 The aci-Nitro Intermediate
• Sommario/riassunto: With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers.A must-have for every biochemist, biophysicist and molecular biologist developing an