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1. |
Record Nr. |
UNINA9910968095703321 |
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Titolo |
A laboratory guide to the mammalian embryo / / edited by David K. Gardner, Michelle Lane, Andrew J. Watson |
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Pubbl/distr/stampa |
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Oxford : , : Oxford University Press, , 2023 |
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ISBN |
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0-19-770144-2 |
1-4294-1517-7 |
9786610481729 |
9786610427888 |
1-280-48172-2 |
1-280-42788-4 |
0-19-803251-X |
1-60256-787-5 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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xiv, 394 p. : ill. (some col.) |
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Collana |
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Oxford scholarship online |
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Disciplina |
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Soggetti |
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Embryology, Experimental |
Embryology, Human |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Previously issued in print: 2004. |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Intro -- Contents -- Contributors -- Abbreviations -- 1. Follicle Development in Vitro -- 2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer -- 3. Culture of the Mammalian Preimplantation Embryo -- 4. Assessment of Preimplantation Embryo Development and Viability -- 5. Micromanipulation of Gametes -- 6. Micromanipulation: Biopsy -- 7. Analysis of Intracellular Ions in Embryos: pH and Calcium -- 8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity -- 9. Metabolic Pathway Activity -- 10. Confocal Imaging of Structural Molecules in Mammalian Gametes -- 11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis -- 12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos -- 13. Microscale RNA Isolation from Mammalian Embryos -- 14. Relative mRNA Transcript Abundance in Early Embryos by Reverse |
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Transcription-Polymerase Chain Reaction -- 15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR -- 16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells -- 17. Antisense Disruption of Gene Expression in the Preimplantation Embryo -- 18. Gene Subtraction and Analysis -- 19. Analysis of Apoptosis in the Preimplantation Embryo -- 20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo -- 21. Human Embryonic Stem Cells -- 22. Spermatogonial Transplantation -- 23. Somatic Cell Nuclear Transfer -- 24. Cryobiology: Slow Freezing Vitrification of Embryos -- Index. |
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Sommario/riassunto |
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Drawing from recent work with embryos from rodents, domestic animals, and primates, this practical guide describes the theory and protocols for performing biochemical, microscopic, and genetic techniques that have been developed and applied at the single-cell level. The techniques are the result of both new research and updates to established proce. |
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2. |
Record Nr. |
UNINA9910954735403321 |
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Autore |
Kernan Alvin B |
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Titolo |
The fruited plain : fables for a postmodern democracy / / Alvin Kernan |
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Pubbl/distr/stampa |
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New Haven, : Yale University Press, c2002 |
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ISBN |
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1-281-72172-7 |
9786611721725 |
0-300-12834-7 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (1 online resource (x, 260 p.)) |
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Disciplina |
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Soggetti |
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Humorous stories, American |
Domestic fiction, American |
Satire, American |
Fables, American |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Bibliographic Level Mode of Issuance: Monograph |
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Nota di contenuto |
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Machine generated contents note: Preface ix -- ONE -- Merry Xmas from the Joads 1 -- TWO -- Majoring in Deconstruction 19 -- THREE -- Religion USA 50 -- FOUR -- Art Lets It All Hang Out 75 -- FIVE -- The Politics Gene 98 -- SIX -- Klutz-Brightgrin H.R. 1984- -- The John Quincy Thud On-Line Biography -- of Great Americans 125 -- SEVEN -- Poshpenny v. Lone Tree State 153 -- EIGHT -- A Long Way Up the Arroyo 178 -- NINE -- Operation Gulliver 205 -- TEN -- Virtual America 227 -- Selected Items from the On-Line Biography -- of Great Americans 251. |
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Sommario/riassunto |
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The beleaguered Joad family of Steinbeck's Grapes of Wrath struggled in an era of disappointed dreams and empty pockets. But how might the grandchildren of that Dust Bowl generation fare in today's more promising times? In this boisterously inventive book Alvin Kernan sends various descendants of the original Joad family on a postmodern journey out of California and into the excesses of American culture at the beginning of the twenty-first century. The experiences of today's Joads are as hilarious as they are discomfiting: they encounter in Kernan's America a world of democracy gone haywire and social institutions in perplexing disarray.In ten satiric episodes, Kernan visits virtually every important American institution-the family, education, religion, art, the military, law courts, sex, science and medicine, politics, and not least television and its advertisements. Unsparing with his barbs, he reveals both the fools and the knaves among us. Kernan's modern-day Joads find themselves in a distorted world where a surplus of democracy not only fails to free its inhabitants but also makes them vulnerable to the machinations of greedy and unscrupulous exploiters. Echoing the voices of such other provocative wits as Evelyn Waugh and Tom Wolfe, Kernan will make you laugh at the absurdity of American culture and-in all likelihood-at yourself. |
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3. |
Record Nr. |
UNINA9910143830603321 |
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Titolo |
De West-Indische gids |
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Pubbl/distr/stampa |
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's-Gravenhage, : M. Nijhoff |
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ISSN |
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Descrizione fisica |
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Soggetti |
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Periodicals. |
Suriname Periodicals |
Netherlands Antilles Periodicals |
Netherlands Antilles |
Suriname |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Periodico |
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Note generali |
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Editors: 1919/20- Janus Boeke and others. |
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