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Practical High-Performance Liquid Chromatography; Contents; Preface to the Fifth Edition; Important and Useful Equations for HPLC; 1 Introduction; 1.1 HPLC: A POWERFUL SEPARATION METHOD; 1.2 A FIRST HPLC EXPERIMENT; 1.3 LIQUID CHROMATOGRAPHIC SEPARATION MODES; 1.4 THE HPLC INSTRUMENT; 1.5 SAFETY IN THE HPLC LABORATORY; 1.6 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY; 1.7 COMPARISON BETWEEN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS; 1.8 UNITS FOR PRESSURE, LENGTH AND VISCOSITY; 1.9 SCIENTIFIC JOURNALS; 1.10 RECOMMENDED BOOKS |
2 Theoretical Principles2.1 THE CHROMATOGRAPHIC PROCESS; 2.2 BAND BROADENING; 2.3 THE CHROMATOGRAM AND ITS PURPORT; 2.4 GRAPHICAL REPRESENTATION OF PEAK PAIRS WITH DIFFERENT DEGREE OF RESOLUTION; 2.5 FACTORS AFFECTING RESOLUTION; 2.6 EXTRA-COLUMN VOLUMES (DEAD VOLUMES); 2.7 TAILING; 2.8 PEAK CAPACITY AND STATISTICAL RESOLUTION PROBABILITY; 2.9 EFFECTS OF TEMPERATURE IN HPLC; 2.10 THE LIMITS OF HPLC; 2.11 HOW TO OBTAIN PEAK CAPACITY; 3 Pumps; 3.1 GENERAL REQUIREMENTS; 3.2 THE SHORT-STROKE PISTON PUMP; 3.3 MAINTENANCE AND REPAIR; 3.4 OTHER PUMP DESIGNS |
4 Preparation of Equipment up to Sample Injection4.1 SELECTION OF THE MOBILE PHASE; 4.2 PREPARATION OF THE MOBILE PHASE; 4.3 GRADIENT SYSTEMS; 4.4 CAPILLARY TUBING; 4.5 FITTINGS; 4.6 SAMPLE INJECTORS; 4.7 SAMPLE SOLUTION AND SAMPLE VOLUME; 5 Solvent Properties; 5.1 TABLE OF ORGANIC SOLVENTS; 5.2 SOLVENT SELECTIVITY; 5.3 MISCIBILITY; 5.4 BUFFERS; 5.5 SHELF LIFE OF MOBILE PHASES; 5.6 THE MIXING CROSS; 6 Detectors; 6.1 GENERAL; 6.2 UV DETECTORS; 6.3 REFRACTIVE INDEX DETECTORS; 6.4 FLUORESCENCE DETECTORS; 6.5 ELECTROCHEMICAL (AMPEROMETRIC) DETECTORS; 6.6 LIGHT-SCATTERING DETECTORS |
6.7 OTHER DETECTORS6.8 MULTIPLE DETECTION; 6.9 INDIRECT DETECTION; 6.10 COUPLING WITH SPECTROSCOPY; 7 Columns and Stationary Phases; 7.1 COLUMNS FOR HPLC; 7.2 PRECOLUMNS; 7.3 GENERAL PROPERTIES OF STATIONARY PHASES; 7.4 SILICA; 7.5 CHEMICALLY MODIFIED SILICA; 7.6 STYRENE-DIVINYLBENZENE; 7.7 SOME OTHER STATIONARY PHASES; 7.8 COLUMN CARE AND REGENERATION; 8 HPLC Column Tests; 8.1 SIMPLE TESTS FOR HPLC COLUMNS; 8.2 DETERMINATION OF PARTICLE SIZE; 8.3 DETERMINATION OF BREAKTHROUGH TIME; 8.4 THE TEST MIXTURE; 8.5 DIMENSIONLESS PARAMETERS FOR HPLC COLUMN CHARACTERIZATION |
8.6 THE VAN DEEMTER EQUATION FROM REDUCED PARAMETERS AND ITS USE IN COLUMN DIAGNOSIS8.7 VAN DEEMTER CURVES AND OTHER COHERENCES; 8.8 DIFFUSION COEFFICIENTS; 9 Adsorption Chromatography: Normal-Phase Chromatography; 9.1 WHAT IS ADSORPTION?; 9.2 THE ELUOTROPIC SERIES; 9.3 SELECTIVITY PROPERTIES OF THE MOBILE PHASE; 9.4 CHOICE AND OPTIMIZATION OF THE MOBILE PHASE; 9.5 APPLICATIONS; 10 Reversed-Phase Chromatography; 10.1 PRINCIPLE; 10.2 MOBILE PHASES IN REVERSED-PHASE CHROMATOGRAPHY; 10.3 SOLVENT SELECTIVITY AND STRENGTH; 10.4 STATIONARY PHASES |
10.5 METHOD DEVELOPMENT IN REVERSED-PHASE CHROMATOGRAPHY |